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Integration of Fluorescence Detection and Image-Based Automated Counting Increases Speed, Sensitivity, and Robustness of Plaque Assays

Plaque assays are used to measure the infectious titer of viral samples. These assays are multi-day and low-throughput and may be subject to analyst variability from biased or subjective manual plaque counting. Typically, on day 1, cells are adhered to plates overnight. On day 2, cells are infected...

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Detalles Bibliográficos
Autores principales: Masci, Allyson L., Menesale, Emily B., Chen, Wei-Chiang, Co, Carl, Lu, Xiaohui, Bergelson, Svetlana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717064/
https://www.ncbi.nlm.nih.gov/pubmed/31489337
http://dx.doi.org/10.1016/j.omtm.2019.07.007