Cargando…
Integration of Fluorescence Detection and Image-Based Automated Counting Increases Speed, Sensitivity, and Robustness of Plaque Assays
Plaque assays are used to measure the infectious titer of viral samples. These assays are multi-day and low-throughput and may be subject to analyst variability from biased or subjective manual plaque counting. Typically, on day 1, cells are adhered to plates overnight. On day 2, cells are infected...
Autores principales: | Masci, Allyson L., Menesale, Emily B., Chen, Wei-Chiang, Co, Carl, Lu, Xiaohui, Bergelson, Svetlana |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717064/ https://www.ncbi.nlm.nih.gov/pubmed/31489337 http://dx.doi.org/10.1016/j.omtm.2019.07.007 |
Ejemplares similares
-
An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting
por: van Remmerden, Yvonne, et al.
Publicado: (2012) -
Machine-learning-based automated quantification machine for virus plaque assay counting
por: Phanomchoeng, Gridsada, et al.
Publicado: (2022) -
An automated cell-counting algorithm for fluorescently-stained cells in migration assays
por: Al-Khazraji, Baraa K, et al.
Publicado: (2011) -
Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis
por: Zielinska, Edyta, et al.
Publicado: (2005) -
Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
por: Kwiatkowski, Allyson, et al.
Publicado: (2020)