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Limited survey on aflatoxin contamination in rice

Aflatoxins (AFS) are toxic and carcinogenic fungal metabolites. Aflatoxin B1 is the most toxic and has been classified as a Group I carcinogen by the International Agency for Research on Cancer (IARC). Samples of imported rice were analyzed for their AFS content. Finley ground rice subsamples were e...

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Autores principales: Al-Zoreky, Najeeb S., Saleh, Farag A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717087/
https://www.ncbi.nlm.nih.gov/pubmed/31485158
http://dx.doi.org/10.1016/j.sjbs.2017.05.010
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author Al-Zoreky, Najeeb S.
Saleh, Farag A.
author_facet Al-Zoreky, Najeeb S.
Saleh, Farag A.
author_sort Al-Zoreky, Najeeb S.
collection PubMed
description Aflatoxins (AFS) are toxic and carcinogenic fungal metabolites. Aflatoxin B1 is the most toxic and has been classified as a Group I carcinogen by the International Agency for Research on Cancer (IARC). Samples of imported rice were analyzed for their AFS content. Finley ground rice subsamples were extracted with water/methanol (100:150 v/v) followed by purification with Immunoaffinity columns (IAC). AFS purified from extracts were determined with RP-HPLC-FLD using post column electrochemical derivatization with a Kobra Cell. Concentrations of aflatoxin B1 and total AFS in test rice samples were ≤0.123 and ≤2.58 µg/kg, respectively. Tween 80 improved recoveries (86 and 106%) of aflatoxin B1 and aflatoxin G1 from brown rice. Recoveries of Aflatoxin B2 and aflatoxin G2 were substantially reduced (non-detected to 27%) by Tween 80 used in IAC cleanup of brown rice extracts. Visible dense growth of Aspergillus parasiticus (food isolate) occurred at 25 °C but higher aflatoxin B1amounts (23.9–39.3 µg/kg) accumulated when the mold grew at 37 °C in rice seeds stored for three weeks. It could be concluded that levels of aflatoxin B1 and total AFS in rice samples were within the permissible amounts of the EU and other international legislations.
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spelling pubmed-67170872019-09-04 Limited survey on aflatoxin contamination in rice Al-Zoreky, Najeeb S. Saleh, Farag A. Saudi J Biol Sci Article Aflatoxins (AFS) are toxic and carcinogenic fungal metabolites. Aflatoxin B1 is the most toxic and has been classified as a Group I carcinogen by the International Agency for Research on Cancer (IARC). Samples of imported rice were analyzed for their AFS content. Finley ground rice subsamples were extracted with water/methanol (100:150 v/v) followed by purification with Immunoaffinity columns (IAC). AFS purified from extracts were determined with RP-HPLC-FLD using post column electrochemical derivatization with a Kobra Cell. Concentrations of aflatoxin B1 and total AFS in test rice samples were ≤0.123 and ≤2.58 µg/kg, respectively. Tween 80 improved recoveries (86 and 106%) of aflatoxin B1 and aflatoxin G1 from brown rice. Recoveries of Aflatoxin B2 and aflatoxin G2 were substantially reduced (non-detected to 27%) by Tween 80 used in IAC cleanup of brown rice extracts. Visible dense growth of Aspergillus parasiticus (food isolate) occurred at 25 °C but higher aflatoxin B1amounts (23.9–39.3 µg/kg) accumulated when the mold grew at 37 °C in rice seeds stored for three weeks. It could be concluded that levels of aflatoxin B1 and total AFS in rice samples were within the permissible amounts of the EU and other international legislations. Elsevier 2019-02 2017-05-20 /pmc/articles/PMC6717087/ /pubmed/31485158 http://dx.doi.org/10.1016/j.sjbs.2017.05.010 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Al-Zoreky, Najeeb S.
Saleh, Farag A.
Limited survey on aflatoxin contamination in rice
title Limited survey on aflatoxin contamination in rice
title_full Limited survey on aflatoxin contamination in rice
title_fullStr Limited survey on aflatoxin contamination in rice
title_full_unstemmed Limited survey on aflatoxin contamination in rice
title_short Limited survey on aflatoxin contamination in rice
title_sort limited survey on aflatoxin contamination in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717087/
https://www.ncbi.nlm.nih.gov/pubmed/31485158
http://dx.doi.org/10.1016/j.sjbs.2017.05.010
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