Cargando…
Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment
Keratinase degrading Bacillus cereus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters we...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717132/ https://www.ncbi.nlm.nih.gov/pubmed/31485181 http://dx.doi.org/10.1016/j.sjbs.2018.10.011 |
_version_ | 1783447502158036992 |
---|---|
author | Arokiyaraj, S. Varghese, R. Ali Ahmed, B. Duraipandiyan, V. Al-Dhabi, N.A. |
author_facet | Arokiyaraj, S. Varghese, R. Ali Ahmed, B. Duraipandiyan, V. Al-Dhabi, N.A. |
author_sort | Arokiyaraj, S. |
collection | PubMed |
description | Keratinase degrading Bacillus cereus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters were optimized using one-variable-at-a-time approach and response surface methodology. Supplementation of 1% lactose supported more keratinase production (120 U/g). Among the selected nitrogen sources, addition of casein significantly enhanced maximum keratinase production (132.5 U/g). Among the ions, manganese chloride significantly enhanced keratinsase production (102.6 U/g), however addition of zinc sulphate and copper sulphate decreased keratinase production. The maximum keratinase production was obtained in the wheat bran medium containing 1% lactose, 0.5% manganese with 80% moisture (292 U/g). Statistics based contour plots were generated to explore the variations in the response surface and to find the relationship between the keratinase yield and the bioprocess conditions. |
format | Online Article Text |
id | pubmed-6717132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-67171322019-09-04 Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment Arokiyaraj, S. Varghese, R. Ali Ahmed, B. Duraipandiyan, V. Al-Dhabi, N.A. Saudi J Biol Sci Article Keratinase degrading Bacillus cereus was isolated from the halophilic environment in Tamilnadu, India and keratinase production was optimized using wheat bran substrate. Of the screened bacterial isolates, four were found to have the ability to produce keratinolytic enzyme. The process parameters were optimized using one-variable-at-a-time approach and response surface methodology. Supplementation of 1% lactose supported more keratinase production (120 U/g). Among the selected nitrogen sources, addition of casein significantly enhanced maximum keratinase production (132.5 U/g). Among the ions, manganese chloride significantly enhanced keratinsase production (102.6 U/g), however addition of zinc sulphate and copper sulphate decreased keratinase production. The maximum keratinase production was obtained in the wheat bran medium containing 1% lactose, 0.5% manganese with 80% moisture (292 U/g). Statistics based contour plots were generated to explore the variations in the response surface and to find the relationship between the keratinase yield and the bioprocess conditions. Elsevier 2019-02 2018-10-16 /pmc/articles/PMC6717132/ /pubmed/31485181 http://dx.doi.org/10.1016/j.sjbs.2018.10.011 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Arokiyaraj, S. Varghese, R. Ali Ahmed, B. Duraipandiyan, V. Al-Dhabi, N.A. Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title | Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title_full | Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title_fullStr | Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title_full_unstemmed | Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title_short | Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment |
title_sort | optimizing the fermentation conditions and enhanced production of keratinase from bacillus cereus isolated from halophilic environment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717132/ https://www.ncbi.nlm.nih.gov/pubmed/31485181 http://dx.doi.org/10.1016/j.sjbs.2018.10.011 |
work_keys_str_mv | AT arokiyarajs optimizingthefermentationconditionsandenhancedproductionofkeratinasefrombacilluscereusisolatedfromhalophilicenvironment AT vargheser optimizingthefermentationconditionsandenhancedproductionofkeratinasefrombacilluscereusisolatedfromhalophilicenvironment AT aliahmedb optimizingthefermentationconditionsandenhancedproductionofkeratinasefrombacilluscereusisolatedfromhalophilicenvironment AT duraipandiyanv optimizingthefermentationconditionsandenhancedproductionofkeratinasefrombacilluscereusisolatedfromhalophilicenvironment AT aldhabina optimizingthefermentationconditionsandenhancedproductionofkeratinasefrombacilluscereusisolatedfromhalophilicenvironment |