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Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies
To identify the enzymes potentially useful for the decolorization of azo dyes, the secretome of the ascomycetous fungus Myrothecium roridum IM6482 was studied by using a bottom-up proteomic approach. Among the identified proteins, the most promising for dye removal was laccase, which decolorized res...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717178/ https://www.ncbi.nlm.nih.gov/pubmed/31286376 http://dx.doi.org/10.1007/s11356-019-05324-6 |
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author | Jasińska, Anna Soboń, Adrian Góralczyk-Bińkowska, Aleksandra Długoński, Jerzy |
author_facet | Jasińska, Anna Soboń, Adrian Góralczyk-Bińkowska, Aleksandra Długoński, Jerzy |
author_sort | Jasińska, Anna |
collection | PubMed |
description | To identify the enzymes potentially useful for the decolorization of azo dyes, the secretome of the ascomycetous fungus Myrothecium roridum IM6482 was studied by using a bottom-up proteomic approach. Among the identified proteins, the most promising for dye removal was laccase, which decolorized respectively, 66, 91, 79, and 80% of Acid Blue 113 (AB 113), Acid Red 27 (AR 27), Direct Blue 14 (DB 14), and Acid Orange 7 (AO 7). The degradation of dyes was enhanced at the wide range of pH from 4 to 8. The addition of redox mediators allowed eliminating AB 113 in concentrations up to 400 mg/L and decolorization of the simulated textile effluent. Microbial toxicity and phytotoxicity tests indicated that dyes are converted into low-toxicity metabolites. This is the first insight into the M. roridum secretome, its identification and its application for removal of select azo dyes. Obtained results extended knowledge concerning biodegradative potential of ascomycetous, ligninolytic fungi and will contribute to the improvement of dye removal by fungi. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-019-05324-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6717178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-67171782019-09-13 Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies Jasińska, Anna Soboń, Adrian Góralczyk-Bińkowska, Aleksandra Długoński, Jerzy Environ Sci Pollut Res Int Research Article To identify the enzymes potentially useful for the decolorization of azo dyes, the secretome of the ascomycetous fungus Myrothecium roridum IM6482 was studied by using a bottom-up proteomic approach. Among the identified proteins, the most promising for dye removal was laccase, which decolorized respectively, 66, 91, 79, and 80% of Acid Blue 113 (AB 113), Acid Red 27 (AR 27), Direct Blue 14 (DB 14), and Acid Orange 7 (AO 7). The degradation of dyes was enhanced at the wide range of pH from 4 to 8. The addition of redox mediators allowed eliminating AB 113 in concentrations up to 400 mg/L and decolorization of the simulated textile effluent. Microbial toxicity and phytotoxicity tests indicated that dyes are converted into low-toxicity metabolites. This is the first insight into the M. roridum secretome, its identification and its application for removal of select azo dyes. Obtained results extended knowledge concerning biodegradative potential of ascomycetous, ligninolytic fungi and will contribute to the improvement of dye removal by fungi. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11356-019-05324-6) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-07-08 2019 /pmc/articles/PMC6717178/ /pubmed/31286376 http://dx.doi.org/10.1007/s11356-019-05324-6 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Article Jasińska, Anna Soboń, Adrian Góralczyk-Bińkowska, Aleksandra Długoński, Jerzy Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title | Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title_full | Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title_fullStr | Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title_full_unstemmed | Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title_short | Analysis of decolorization potential of Myrothecium roridum in the light of its secretome and toxicological studies |
title_sort | analysis of decolorization potential of myrothecium roridum in the light of its secretome and toxicological studies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717178/ https://www.ncbi.nlm.nih.gov/pubmed/31286376 http://dx.doi.org/10.1007/s11356-019-05324-6 |
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