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Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver

AIM: To determine whether the use of a mixed polymeric micelle delivery system based on vitamin E succinate (VES)-grafted-chitosan oligosaccharide (CSO)/VES-grafted-chitosan (CS) mixed micelles (VES-g-CSO/VES-g-CS MM) enhances the delivery of C-DMSA, a theranostic fluorescent probe, for Hg(2+) detec...

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Autores principales: Wei, Binghui, He, Muye, Cai, Xiaoran, Hou, Xinyu, Wang, Yujie, Chen, Jiaojiao, Lan, Minbo, Chen, Yanzuo, Lou, Kaiyan, Gao, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717732/
https://www.ncbi.nlm.nih.gov/pubmed/31695366
http://dx.doi.org/10.2147/IJN.S213084
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author Wei, Binghui
He, Muye
Cai, Xiaoran
Hou, Xinyu
Wang, Yujie
Chen, Jiaojiao
Lan, Minbo
Chen, Yanzuo
Lou, Kaiyan
Gao, Feng
author_facet Wei, Binghui
He, Muye
Cai, Xiaoran
Hou, Xinyu
Wang, Yujie
Chen, Jiaojiao
Lan, Minbo
Chen, Yanzuo
Lou, Kaiyan
Gao, Feng
author_sort Wei, Binghui
collection PubMed
description AIM: To determine whether the use of a mixed polymeric micelle delivery system based on vitamin E succinate (VES)-grafted-chitosan oligosaccharide (CSO)/VES-grafted-chitosan (CS) mixed micelles (VES-g-CSO/VES-g-CS MM) enhances the delivery of C-DMSA, a theranostic fluorescent probe, for Hg(2+) detection and detoxification in vitro and in vivo. METHODS: Mixed micelles self-assembled from two polymers, VES-g-CSO and VES-g-CS, were used to load C-DMSA and afforded C-DMSA@VES-g-CSO/VES-g-CS MM for cell and in vivo applications. Fluorescence microscopy was used to assess C-DMSA cellular uptake and Hg(2+) detection in L929 cells. C-DMSA@VES-g-CSO/VES-g-CS MM was then administered intravenously. Hg(2+) detection was assessed by fluorescence microscopy in terms of bio-distribution while detoxification efficacy in Hg(2+)-poisoned rat models was evaluated in terms of mercury contents in blood and in liver. RESULTS: The C-DMSA loaded mixed micelles, C-DMSA@VES-g-CSO/VES-g-CS MM, significantly enhanced cellular uptake and detoxification efficacy of C-DMSA in Hg(2+) pretreated human L929 cells. Evidence from the reduction of liver coefficient, mercury contents in liver and blood, alanine transaminase and aspartate transaminase activities in Hg(2+) poisoned SD rats treated with the mixed micelles strongly supported that the micelles were effective for Hg(2+) detoxification in vivo. Furthermore, ex vivo fluorescence imaging experiments also supported enhanced Hg(2+) detection in rat liver. CONCLUSION: The mixed polymeric micelle delivery system could significantly enhance cell uptake and efficacy of a theranostic probe for Hg(2+) detection and detoxification treatment in vitro and in vivo. Moreover, this nanoparticle drug delivery system could achieve targeted detection and detoxification in liver.
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spelling pubmed-67177322019-11-06 Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver Wei, Binghui He, Muye Cai, Xiaoran Hou, Xinyu Wang, Yujie Chen, Jiaojiao Lan, Minbo Chen, Yanzuo Lou, Kaiyan Gao, Feng Int J Nanomedicine Original Research AIM: To determine whether the use of a mixed polymeric micelle delivery system based on vitamin E succinate (VES)-grafted-chitosan oligosaccharide (CSO)/VES-grafted-chitosan (CS) mixed micelles (VES-g-CSO/VES-g-CS MM) enhances the delivery of C-DMSA, a theranostic fluorescent probe, for Hg(2+) detection and detoxification in vitro and in vivo. METHODS: Mixed micelles self-assembled from two polymers, VES-g-CSO and VES-g-CS, were used to load C-DMSA and afforded C-DMSA@VES-g-CSO/VES-g-CS MM for cell and in vivo applications. Fluorescence microscopy was used to assess C-DMSA cellular uptake and Hg(2+) detection in L929 cells. C-DMSA@VES-g-CSO/VES-g-CS MM was then administered intravenously. Hg(2+) detection was assessed by fluorescence microscopy in terms of bio-distribution while detoxification efficacy in Hg(2+)-poisoned rat models was evaluated in terms of mercury contents in blood and in liver. RESULTS: The C-DMSA loaded mixed micelles, C-DMSA@VES-g-CSO/VES-g-CS MM, significantly enhanced cellular uptake and detoxification efficacy of C-DMSA in Hg(2+) pretreated human L929 cells. Evidence from the reduction of liver coefficient, mercury contents in liver and blood, alanine transaminase and aspartate transaminase activities in Hg(2+) poisoned SD rats treated with the mixed micelles strongly supported that the micelles were effective for Hg(2+) detoxification in vivo. Furthermore, ex vivo fluorescence imaging experiments also supported enhanced Hg(2+) detection in rat liver. CONCLUSION: The mixed polymeric micelle delivery system could significantly enhance cell uptake and efficacy of a theranostic probe for Hg(2+) detection and detoxification treatment in vitro and in vivo. Moreover, this nanoparticle drug delivery system could achieve targeted detection and detoxification in liver. Dove 2019-08-27 /pmc/articles/PMC6717732/ /pubmed/31695366 http://dx.doi.org/10.2147/IJN.S213084 Text en © 2019 Wei et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wei, Binghui
He, Muye
Cai, Xiaoran
Hou, Xinyu
Wang, Yujie
Chen, Jiaojiao
Lan, Minbo
Chen, Yanzuo
Lou, Kaiyan
Gao, Feng
Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title_full Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title_fullStr Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title_full_unstemmed Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title_short Vitamin E succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with C-DMSA for Hg(2+) detection and detoxification in rat liver
title_sort vitamin e succinate-grafted-chitosan/chitosan oligosaccharide mixed micelles loaded with c-dmsa for hg(2+) detection and detoxification in rat liver
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717732/
https://www.ncbi.nlm.nih.gov/pubmed/31695366
http://dx.doi.org/10.2147/IJN.S213084
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