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Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenter...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chang Gung University
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717750/ https://www.ncbi.nlm.nih.gov/pubmed/31466712 http://dx.doi.org/10.1016/j.bj.2019.01.009 |
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author | Hash, Sara Martinez-Viedma, M. Pilar Fung, Fred Han, Jee Eun Yang, Paul Wong, Charlene Doraisamy, Loganathan Menon, Suresh Lightner, Donald |
author_facet | Hash, Sara Martinez-Viedma, M. Pilar Fung, Fred Han, Jee Eun Yang, Paul Wong, Charlene Doraisamy, Loganathan Menon, Suresh Lightner, Donald |
author_sort | Hash, Sara |
collection | PubMed |
description | BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. METHODS: A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10(5) CFU/ml) with Molecular Mirroring using iron nanoparticles coated with target-specific biomarkers capable of binding to DNA of the target microorganism. The NMR system generates a signal (in milliseconds) by measuring NMR spin–spin relaxation time T(2), which correlates with the amount of microorganism DNA. RESULTS: Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10(5)–10(8) CFU/ml) using two DNA extraction methods. In addition, the NMR biosensor system can detect a wide range of microorganism DNAs in different matrices within a short period of time. CONCLUSION: NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The “hybrid” technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety. |
format | Online Article Text |
id | pubmed-6717750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Chang Gung University |
record_format | MEDLINE/PubMed |
spelling | pubmed-67177502019-09-06 Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus Hash, Sara Martinez-Viedma, M. Pilar Fung, Fred Han, Jee Eun Yang, Paul Wong, Charlene Doraisamy, Loganathan Menon, Suresh Lightner, Donald Biomed J Original Article BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. METHODS: A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10(5) CFU/ml) with Molecular Mirroring using iron nanoparticles coated with target-specific biomarkers capable of binding to DNA of the target microorganism. The NMR system generates a signal (in milliseconds) by measuring NMR spin–spin relaxation time T(2), which correlates with the amount of microorganism DNA. RESULTS: Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10(5)–10(8) CFU/ml) using two DNA extraction methods. In addition, the NMR biosensor system can detect a wide range of microorganism DNAs in different matrices within a short period of time. CONCLUSION: NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The “hybrid” technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety. Chang Gung University 2019-06 2019-07-15 /pmc/articles/PMC6717750/ /pubmed/31466712 http://dx.doi.org/10.1016/j.bj.2019.01.009 Text en © 2019 Chang Gung University. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Hash, Sara Martinez-Viedma, M. Pilar Fung, Fred Han, Jee Eun Yang, Paul Wong, Charlene Doraisamy, Loganathan Menon, Suresh Lightner, Donald Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title | Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title_full | Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title_fullStr | Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title_full_unstemmed | Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title_short | Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus |
title_sort | nuclear magnetic resonance biosensor for rapid detection of vibrio parahaemolyticus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717750/ https://www.ncbi.nlm.nih.gov/pubmed/31466712 http://dx.doi.org/10.1016/j.bj.2019.01.009 |
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