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Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus

BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenter...

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Autores principales: Hash, Sara, Martinez-Viedma, M. Pilar, Fung, Fred, Han, Jee Eun, Yang, Paul, Wong, Charlene, Doraisamy, Loganathan, Menon, Suresh, Lightner, Donald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chang Gung University 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717750/
https://www.ncbi.nlm.nih.gov/pubmed/31466712
http://dx.doi.org/10.1016/j.bj.2019.01.009
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author Hash, Sara
Martinez-Viedma, M. Pilar
Fung, Fred
Han, Jee Eun
Yang, Paul
Wong, Charlene
Doraisamy, Loganathan
Menon, Suresh
Lightner, Donald
author_facet Hash, Sara
Martinez-Viedma, M. Pilar
Fung, Fred
Han, Jee Eun
Yang, Paul
Wong, Charlene
Doraisamy, Loganathan
Menon, Suresh
Lightner, Donald
author_sort Hash, Sara
collection PubMed
description BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. METHODS: A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10(5) CFU/ml) with Molecular Mirroring using iron nanoparticles coated with target-specific biomarkers capable of binding to DNA of the target microorganism. The NMR system generates a signal (in milliseconds) by measuring NMR spin–spin relaxation time T(2), which correlates with the amount of microorganism DNA. RESULTS: Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10(5)–10(8) CFU/ml) using two DNA extraction methods. In addition, the NMR biosensor system can detect a wide range of microorganism DNAs in different matrices within a short period of time. CONCLUSION: NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The “hybrid” technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety.
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spelling pubmed-67177502019-09-06 Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus Hash, Sara Martinez-Viedma, M. Pilar Fung, Fred Han, Jee Eun Yang, Paul Wong, Charlene Doraisamy, Loganathan Menon, Suresh Lightner, Donald Biomed J Original Article BACKGROUND: Vibrio parahaemolyticus is a Gram-negative bacterium widely distributed in marine environments and a well-recognized invertebrate pathogen frequently isolated from seafood. V. parahaemolyticus may also spread into humans, via contaminated, raw, or undercooked seafood, causing gastroenteritis and diarrhea. METHODS: A Nuclear Magnetic Resonance (NMR)-based detection system was used to detect pathogenic levels of this microorganism (10(5) CFU/ml) with Molecular Mirroring using iron nanoparticles coated with target-specific biomarkers capable of binding to DNA of the target microorganism. The NMR system generates a signal (in milliseconds) by measuring NMR spin–spin relaxation time T(2), which correlates with the amount of microorganism DNA. RESULTS: Compared with conventional microbiology techniques such as real-time PCR (qPCR), the NMR biosensor showed similar limits of detection (LOD) at different concentrations (10(5)–10(8) CFU/ml) using two DNA extraction methods. In addition, the NMR biosensor system can detect a wide range of microorganism DNAs in different matrices within a short period of time. CONCLUSION: NMR biosensor represents a potential tool for diagnostic and quality control to ensure microbial pathogens such as V. parahaemolyticus are not the cause of infection. The “hybrid” technology (NMR and nanoparticle application) opens a new platform for detecting other microbial pathogens that have impacted human health, animal health and food safety. Chang Gung University 2019-06 2019-07-15 /pmc/articles/PMC6717750/ /pubmed/31466712 http://dx.doi.org/10.1016/j.bj.2019.01.009 Text en © 2019 Chang Gung University. Publishing services by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Hash, Sara
Martinez-Viedma, M. Pilar
Fung, Fred
Han, Jee Eun
Yang, Paul
Wong, Charlene
Doraisamy, Loganathan
Menon, Suresh
Lightner, Donald
Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title_full Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title_fullStr Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title_full_unstemmed Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title_short Nuclear magnetic resonance biosensor for rapid detection of Vibrio parahaemolyticus
title_sort nuclear magnetic resonance biosensor for rapid detection of vibrio parahaemolyticus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717750/
https://www.ncbi.nlm.nih.gov/pubmed/31466712
http://dx.doi.org/10.1016/j.bj.2019.01.009
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