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Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response

Cassava (Manihot esculenta) is a major staple food, animal feed and energy crop in the tropics and subtropics. It is one of the most drought-tolerant crops, however, the mechanisms of cassava drought tolerance remain unclear. Abscisic acid (ABA)-responsive element (ABRE)-binding factors (ABFs) are t...

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Autores principales: Feng, Ren-Jun, Ren, Meng-Yun, Lu, Li-Fang, Peng, Ming, Guan, Xiao, Zhou, Deng-Bo, Zhang, Miao-Yi, Qi, Deng-Feng, Li, Kai, Tang, Wen, Yun, Tian-Yan, Chen, Yu-Feng, Wang, Fei, Zhang, Dun, Shen, Qi, Liang, Ping, Zhang, Yin-Dong, Xie, Jiang-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718394/
https://www.ncbi.nlm.nih.gov/pubmed/31477771
http://dx.doi.org/10.1038/s41598-019-49083-3
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author Feng, Ren-Jun
Ren, Meng-Yun
Lu, Li-Fang
Peng, Ming
Guan, Xiao
Zhou, Deng-Bo
Zhang, Miao-Yi
Qi, Deng-Feng
Li, Kai
Tang, Wen
Yun, Tian-Yan
Chen, Yu-Feng
Wang, Fei
Zhang, Dun
Shen, Qi
Liang, Ping
Zhang, Yin-Dong
Xie, Jiang-Hui
author_facet Feng, Ren-Jun
Ren, Meng-Yun
Lu, Li-Fang
Peng, Ming
Guan, Xiao
Zhou, Deng-Bo
Zhang, Miao-Yi
Qi, Deng-Feng
Li, Kai
Tang, Wen
Yun, Tian-Yan
Chen, Yu-Feng
Wang, Fei
Zhang, Dun
Shen, Qi
Liang, Ping
Zhang, Yin-Dong
Xie, Jiang-Hui
author_sort Feng, Ren-Jun
collection PubMed
description Cassava (Manihot esculenta) is a major staple food, animal feed and energy crop in the tropics and subtropics. It is one of the most drought-tolerant crops, however, the mechanisms of cassava drought tolerance remain unclear. Abscisic acid (ABA)-responsive element (ABRE)-binding factors (ABFs) are transcription factors that regulate expression of target genes involved in plant tolerance to drought, high salinity, and osmotic stress by binding ABRE cis-elements in the promoter regions of these genes. However, there is little information about ABF genes in cassava. A comprehensive analysis of Manihot esculenta ABFs (MeABFs) described the phylogeny, genome location, cis-acting elements, expression profiles, and regulatory relationship between these factors and Manihot esculenta betaine aldehyde dehydrogenase genes (MeBADHs). Here we conducted genome-wide searches and subsequent molecular cloning to identify seven MeABFs that are distributed unevenly across six chromosomes in cassava. These MeABFs can be clustered into three groups according to their phylogenetic relationships to their Arabidopsis (Arabidopsis thaliana) counterparts. Analysis of the 5′-upstream region of MeABFs revealed putative cis-acting elements related to hormone signaling, stress, light, and circadian clock. MeABF expression profiles displayed clear differences among leaf, stem, root, and tuberous root tissues under non-stress and drought, osmotic, or salt stress conditions. Drought stress in cassava leaves and roots, osmotic stress in tuberous roots, and salt stress in stems induced expression of the highest number of MeABFs showing significantly elevated expression. The glycine betaine (GB) content of cassava leaves also was elevated after drought, osmotic, or salt stress treatments. BADH1 is involved in GB synthesis. We show that MeBADH1 promoter sequences contained ABREs and that MeBADH1 expression correlated with MeABF expression profiles in cassava leaves after the three stress treatments. Taken together, these results suggest that in response to various dehydration stresses, MeABFs in cassava may activate transcriptional expression of MeBADH1 by binding the MeBADH1 promoter that in turn promotes GB biosynthesis and accumulation via an increase in MeBADH1 gene expression levels and MeBADH1 enzymatic activity. These responses protect cells against dehydration stresses by preserving an osmotic balance that enhances cassava tolerance to dehydration stresses.
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spelling pubmed-67183942019-09-17 Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response Feng, Ren-Jun Ren, Meng-Yun Lu, Li-Fang Peng, Ming Guan, Xiao Zhou, Deng-Bo Zhang, Miao-Yi Qi, Deng-Feng Li, Kai Tang, Wen Yun, Tian-Yan Chen, Yu-Feng Wang, Fei Zhang, Dun Shen, Qi Liang, Ping Zhang, Yin-Dong Xie, Jiang-Hui Sci Rep Article Cassava (Manihot esculenta) is a major staple food, animal feed and energy crop in the tropics and subtropics. It is one of the most drought-tolerant crops, however, the mechanisms of cassava drought tolerance remain unclear. Abscisic acid (ABA)-responsive element (ABRE)-binding factors (ABFs) are transcription factors that regulate expression of target genes involved in plant tolerance to drought, high salinity, and osmotic stress by binding ABRE cis-elements in the promoter regions of these genes. However, there is little information about ABF genes in cassava. A comprehensive analysis of Manihot esculenta ABFs (MeABFs) described the phylogeny, genome location, cis-acting elements, expression profiles, and regulatory relationship between these factors and Manihot esculenta betaine aldehyde dehydrogenase genes (MeBADHs). Here we conducted genome-wide searches and subsequent molecular cloning to identify seven MeABFs that are distributed unevenly across six chromosomes in cassava. These MeABFs can be clustered into three groups according to their phylogenetic relationships to their Arabidopsis (Arabidopsis thaliana) counterparts. Analysis of the 5′-upstream region of MeABFs revealed putative cis-acting elements related to hormone signaling, stress, light, and circadian clock. MeABF expression profiles displayed clear differences among leaf, stem, root, and tuberous root tissues under non-stress and drought, osmotic, or salt stress conditions. Drought stress in cassava leaves and roots, osmotic stress in tuberous roots, and salt stress in stems induced expression of the highest number of MeABFs showing significantly elevated expression. The glycine betaine (GB) content of cassava leaves also was elevated after drought, osmotic, or salt stress treatments. BADH1 is involved in GB synthesis. We show that MeBADH1 promoter sequences contained ABREs and that MeBADH1 expression correlated with MeABF expression profiles in cassava leaves after the three stress treatments. Taken together, these results suggest that in response to various dehydration stresses, MeABFs in cassava may activate transcriptional expression of MeBADH1 by binding the MeBADH1 promoter that in turn promotes GB biosynthesis and accumulation via an increase in MeBADH1 gene expression levels and MeBADH1 enzymatic activity. These responses protect cells against dehydration stresses by preserving an osmotic balance that enhances cassava tolerance to dehydration stresses. Nature Publishing Group UK 2019-09-02 /pmc/articles/PMC6718394/ /pubmed/31477771 http://dx.doi.org/10.1038/s41598-019-49083-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Feng, Ren-Jun
Ren, Meng-Yun
Lu, Li-Fang
Peng, Ming
Guan, Xiao
Zhou, Deng-Bo
Zhang, Miao-Yi
Qi, Deng-Feng
Li, Kai
Tang, Wen
Yun, Tian-Yan
Chen, Yu-Feng
Wang, Fei
Zhang, Dun
Shen, Qi
Liang, Ping
Zhang, Yin-Dong
Xie, Jiang-Hui
Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title_full Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title_fullStr Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title_full_unstemmed Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title_short Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response
title_sort involvement of abscisic acid-responsive element-binding factors in cassava (manihot esculenta) dehydration stress response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718394/
https://www.ncbi.nlm.nih.gov/pubmed/31477771
http://dx.doi.org/10.1038/s41598-019-49083-3
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