Cargando…
High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK
Background: Human Immunodeficiency Virus 1 (HIV-1) and Mycobacterium Tuberculosis (Mtb) co-infected patients are commonly at risk of immune reconstitution inflammatory syndrome (IRIS) when initiating antiretroviral treatment (ART). Evidence indicates that innate immunity plays a role in TB-IRIS. Her...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718564/ https://www.ncbi.nlm.nih.gov/pubmed/31507608 http://dx.doi.org/10.3389/fimmu.2019.02018 |
_version_ | 1783447745856536576 |
---|---|
author | Pean, Polidy Nouhin, Janin Ratana, Meng Madec, Yoann Borand, Laurence Marcy, Olivier Laureillard, Didier Fernandez, Marcelo Barré-Sinoussi, Françoise Weiss, Laurence Scott-Algara, Daniel |
author_facet | Pean, Polidy Nouhin, Janin Ratana, Meng Madec, Yoann Borand, Laurence Marcy, Olivier Laureillard, Didier Fernandez, Marcelo Barré-Sinoussi, Françoise Weiss, Laurence Scott-Algara, Daniel |
author_sort | Pean, Polidy |
collection | PubMed |
description | Background: Human Immunodeficiency Virus 1 (HIV-1) and Mycobacterium Tuberculosis (Mtb) co-infected patients are commonly at risk of immune reconstitution inflammatory syndrome (IRIS) when initiating antiretroviral treatment (ART). Evidence indicates that innate immunity plays a role in TB-IRIS. Here, we evaluate the phenotype of Gamma-delta (γδ) T cells and invariant Natural Killer (iNK) T cells in tuberculosis-associated IRIS. Methods: Forty-eight HIV+/TB+ patients (21 IRIS) and three control groups: HIV–/TB– (HD, n = 11), HIV+/TB– (n = 26), and HIV–/TB+ (n = 22) were studied. Samples were taken at ART initiation (week 2 of anti-tuberculosis treatment) and at the diagnosis of IRIS for HIV+/TB+; before ART for HIV+/TB-, and at week 2 of anti-tuberculosis treatment for HIV–/TB+ patients. γδ T cells and Invariant natural killer T (iNKT) cells were analyzed by flow cytometry. Results: Before ART, IRIS, and non-IRIS patients showed a similar proportion of γδ(pos) T and iNKT cells. HLA-DR on γδ(pos) T cells and δ2(pos)γδ(pos) T cells was significantly higher in TB-IRIS vs. non-IRIS patients and controls (p < 0.0001). NKG2D expression on γδ(pos) T cells and the δ2(pos)γδ(pos) T cell subset was lower in HIV+/TB+ patients than controls. CD158a expression on γδ(pos) T cells was higher in TB-IRIS than non-IRIS (p = 0.02), HIV+/TB–, and HIV–/TB- patients. Conclusion: The higher activation of γδ(pos)T cells and the γδ2(pos)γδ(pos) T cell subset suggests that γδ T cells may play a role in the pathogenesis of TB-IRIS. |
format | Online Article Text |
id | pubmed-6718564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67185642019-09-10 High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK Pean, Polidy Nouhin, Janin Ratana, Meng Madec, Yoann Borand, Laurence Marcy, Olivier Laureillard, Didier Fernandez, Marcelo Barré-Sinoussi, Françoise Weiss, Laurence Scott-Algara, Daniel Front Immunol Immunology Background: Human Immunodeficiency Virus 1 (HIV-1) and Mycobacterium Tuberculosis (Mtb) co-infected patients are commonly at risk of immune reconstitution inflammatory syndrome (IRIS) when initiating antiretroviral treatment (ART). Evidence indicates that innate immunity plays a role in TB-IRIS. Here, we evaluate the phenotype of Gamma-delta (γδ) T cells and invariant Natural Killer (iNK) T cells in tuberculosis-associated IRIS. Methods: Forty-eight HIV+/TB+ patients (21 IRIS) and three control groups: HIV–/TB– (HD, n = 11), HIV+/TB– (n = 26), and HIV–/TB+ (n = 22) were studied. Samples were taken at ART initiation (week 2 of anti-tuberculosis treatment) and at the diagnosis of IRIS for HIV+/TB+; before ART for HIV+/TB-, and at week 2 of anti-tuberculosis treatment for HIV–/TB+ patients. γδ T cells and Invariant natural killer T (iNKT) cells were analyzed by flow cytometry. Results: Before ART, IRIS, and non-IRIS patients showed a similar proportion of γδ(pos) T and iNKT cells. HLA-DR on γδ(pos) T cells and δ2(pos)γδ(pos) T cells was significantly higher in TB-IRIS vs. non-IRIS patients and controls (p < 0.0001). NKG2D expression on γδ(pos) T cells and the δ2(pos)γδ(pos) T cell subset was lower in HIV+/TB+ patients than controls. CD158a expression on γδ(pos) T cells was higher in TB-IRIS than non-IRIS (p = 0.02), HIV+/TB–, and HIV–/TB- patients. Conclusion: The higher activation of γδ(pos)T cells and the γδ2(pos)γδ(pos) T cell subset suggests that γδ T cells may play a role in the pathogenesis of TB-IRIS. Frontiers Media S.A. 2019-08-27 /pmc/articles/PMC6718564/ /pubmed/31507608 http://dx.doi.org/10.3389/fimmu.2019.02018 Text en Copyright © 2019 Pean, Nouhin, Ratana, Madec, Borand, Marcy, Laureillard, Fernandez, Barré-Sinoussi, Weiss and Scott-Algara. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Pean, Polidy Nouhin, Janin Ratana, Meng Madec, Yoann Borand, Laurence Marcy, Olivier Laureillard, Didier Fernandez, Marcelo Barré-Sinoussi, Françoise Weiss, Laurence Scott-Algara, Daniel High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title | High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title_full | High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title_fullStr | High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title_full_unstemmed | High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title_short | High Activation of γδ T Cells and the γδ2(pos) T-Cell Subset Is Associated With the Onset of Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome, ANRS 12153 CAPRI NK |
title_sort | high activation of γδ t cells and the γδ2(pos) t-cell subset is associated with the onset of tuberculosis-associated immune reconstitution inflammatory syndrome, anrs 12153 capri nk |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718564/ https://www.ncbi.nlm.nih.gov/pubmed/31507608 http://dx.doi.org/10.3389/fimmu.2019.02018 |
work_keys_str_mv | AT peanpolidy highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT nouhinjanin highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT ratanameng highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT madecyoann highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT borandlaurence highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT marcyolivier highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT laureillarddidier highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT fernandezmarcelo highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT barresinoussifrancoise highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT weisslaurence highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink AT scottalgaradaniel highactivationofgdtcellsandthegd2postcellsubsetisassociatedwiththeonsetoftuberculosisassociatedimmunereconstitutioninflammatorysyndromeanrs12153caprink |