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(Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis
To extend life expectancy and ensure healthy aging, it is crucial to prevent and minimize age‐induced skeletal muscle atrophy, also known as sarcopenia. However, the disease's molecular mechanism remains unclear. The age‐related Wnt/β‐catenin signaling pathway has been recently shown to be acti...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718617/ https://www.ncbi.nlm.nih.gov/pubmed/31282603 http://dx.doi.org/10.1111/acel.12991 |
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author | Yoshida, Naohiro Endo, Jin Kinouchi, Kenichiro Kitakata, Hiroki Moriyama, Hidenori Kataoka, Masaharu Yamamoto, Tsunehisa Shirakawa, Kohsuke Morimoto, Satoshi Nishiyama, Akira Hashiguchi, Akihiro Higuchi, Itsuro Fukuda, Keiichi Ichihara, Atsuhiro Sano, Motoaki |
author_facet | Yoshida, Naohiro Endo, Jin Kinouchi, Kenichiro Kitakata, Hiroki Moriyama, Hidenori Kataoka, Masaharu Yamamoto, Tsunehisa Shirakawa, Kohsuke Morimoto, Satoshi Nishiyama, Akira Hashiguchi, Akihiro Higuchi, Itsuro Fukuda, Keiichi Ichihara, Atsuhiro Sano, Motoaki |
author_sort | Yoshida, Naohiro |
collection | PubMed |
description | To extend life expectancy and ensure healthy aging, it is crucial to prevent and minimize age‐induced skeletal muscle atrophy, also known as sarcopenia. However, the disease's molecular mechanism remains unclear. The age‐related Wnt/β‐catenin signaling pathway has been recently shown to be activated by the (pro)renin receptor ((P)RR). We report here that (P)RR expression was increased in the atrophied skeletal muscles of aged mice and humans. Therefore, we developed a gain‐of‐function model of age‐related sarcopenia via transgenic expression of (P)RR under control of the CAG promoter. Consistent with our hypothesis, (P)RR‐Tg mice died early and exhibited muscle atrophy with histological features of sarcopenia. Moreover, Wnt/β‐catenin signaling was activated and the regenerative capacity of muscle progenitor cells after cardiotoxin injury was impaired due to cell fusion failure in (P)RR‐Tg mice. In vitro forced expression of (P)RR protein in C2C12 myoblast cells suppressed myotube formation by activating Wnt/β‐catenin signaling. Administration of Dickkopf‐related protein 1, an inhibitor of Wnt/β‐catenin signaling, and anti‐(P)RR neutralizing antibody, which inhibits binding of (P)RR to the Wnt receptor, significantly improved sarcopenia in (P)RR‐Tg mice. Furthermore, the use of anti‐(P)RR neutralizing antibodies significantly improved the regenerative ability of skeletal muscle in aged mice. Finally, we show that Yes‐associated protein (YAP) signaling, which is coordinately regulated by Wnt/β‐catenin, contributed to the development of (P)RR‐induced sarcopenia. The present study demonstrates the use of (P)RR‐Tg mice as a novel sarcopenia model, and shows that (P)RR‐Wnt‐YAP signaling plays a pivotal role in the pathogenesis of this disease. |
format | Online Article Text |
id | pubmed-6718617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67186172019-10-01 (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis Yoshida, Naohiro Endo, Jin Kinouchi, Kenichiro Kitakata, Hiroki Moriyama, Hidenori Kataoka, Masaharu Yamamoto, Tsunehisa Shirakawa, Kohsuke Morimoto, Satoshi Nishiyama, Akira Hashiguchi, Akihiro Higuchi, Itsuro Fukuda, Keiichi Ichihara, Atsuhiro Sano, Motoaki Aging Cell Original Articles To extend life expectancy and ensure healthy aging, it is crucial to prevent and minimize age‐induced skeletal muscle atrophy, also known as sarcopenia. However, the disease's molecular mechanism remains unclear. The age‐related Wnt/β‐catenin signaling pathway has been recently shown to be activated by the (pro)renin receptor ((P)RR). We report here that (P)RR expression was increased in the atrophied skeletal muscles of aged mice and humans. Therefore, we developed a gain‐of‐function model of age‐related sarcopenia via transgenic expression of (P)RR under control of the CAG promoter. Consistent with our hypothesis, (P)RR‐Tg mice died early and exhibited muscle atrophy with histological features of sarcopenia. Moreover, Wnt/β‐catenin signaling was activated and the regenerative capacity of muscle progenitor cells after cardiotoxin injury was impaired due to cell fusion failure in (P)RR‐Tg mice. In vitro forced expression of (P)RR protein in C2C12 myoblast cells suppressed myotube formation by activating Wnt/β‐catenin signaling. Administration of Dickkopf‐related protein 1, an inhibitor of Wnt/β‐catenin signaling, and anti‐(P)RR neutralizing antibody, which inhibits binding of (P)RR to the Wnt receptor, significantly improved sarcopenia in (P)RR‐Tg mice. Furthermore, the use of anti‐(P)RR neutralizing antibodies significantly improved the regenerative ability of skeletal muscle in aged mice. Finally, we show that Yes‐associated protein (YAP) signaling, which is coordinately regulated by Wnt/β‐catenin, contributed to the development of (P)RR‐induced sarcopenia. The present study demonstrates the use of (P)RR‐Tg mice as a novel sarcopenia model, and shows that (P)RR‐Wnt‐YAP signaling plays a pivotal role in the pathogenesis of this disease. John Wiley and Sons Inc. 2019-07-08 2019-10 /pmc/articles/PMC6718617/ /pubmed/31282603 http://dx.doi.org/10.1111/acel.12991 Text en © 2019 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Yoshida, Naohiro Endo, Jin Kinouchi, Kenichiro Kitakata, Hiroki Moriyama, Hidenori Kataoka, Masaharu Yamamoto, Tsunehisa Shirakawa, Kohsuke Morimoto, Satoshi Nishiyama, Akira Hashiguchi, Akihiro Higuchi, Itsuro Fukuda, Keiichi Ichihara, Atsuhiro Sano, Motoaki (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title | (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title_full | (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title_fullStr | (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title_full_unstemmed | (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title_short | (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis |
title_sort | (pro)renin receptor accelerates development of sarcopenia via activation of wnt/yap signaling axis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718617/ https://www.ncbi.nlm.nih.gov/pubmed/31282603 http://dx.doi.org/10.1111/acel.12991 |
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