A novel magnet-based scratch method for standardisation of wound-healing assays

A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is aff...

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Detalles Bibliográficos
Autores principales: Fenu, M., Bettermann, T., Vogl, C., Darwish-Miranda, N., Schramel, J., Jenner, F., Ribitsch, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718675/
https://www.ncbi.nlm.nih.gov/pubmed/31477739
http://dx.doi.org/10.1038/s41598-019-48930-7
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author Fenu, M.
Bettermann, T.
Vogl, C.
Darwish-Miranda, N.
Schramel, J.
Jenner, F.
Ribitsch, I.
author_facet Fenu, M.
Bettermann, T.
Vogl, C.
Darwish-Miranda, N.
Schramel, J.
Jenner, F.
Ribitsch, I.
author_sort Fenu, M.
collection PubMed
description A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories.
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spelling pubmed-67186752019-09-17 A novel magnet-based scratch method for standardisation of wound-healing assays Fenu, M. Bettermann, T. Vogl, C. Darwish-Miranda, N. Schramel, J. Jenner, F. Ribitsch, I. Sci Rep Article A novel magnetic scratch method achieves repeatability, reproducibility and geometric control greater than pipette scratch assays and closely approximating the precision of cell exclusion assays while inducing the cell injury inherently necessary for wound healing assays. The magnetic scratch is affordable, easily implemented and standardisable and thus may contribute toward better comparability of data generated in different studies and laboratories. Nature Publishing Group UK 2019-09-02 /pmc/articles/PMC6718675/ /pubmed/31477739 http://dx.doi.org/10.1038/s41598-019-48930-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fenu, M.
Bettermann, T.
Vogl, C.
Darwish-Miranda, N.
Schramel, J.
Jenner, F.
Ribitsch, I.
A novel magnet-based scratch method for standardisation of wound-healing assays
title A novel magnet-based scratch method for standardisation of wound-healing assays
title_full A novel magnet-based scratch method for standardisation of wound-healing assays
title_fullStr A novel magnet-based scratch method for standardisation of wound-healing assays
title_full_unstemmed A novel magnet-based scratch method for standardisation of wound-healing assays
title_short A novel magnet-based scratch method for standardisation of wound-healing assays
title_sort novel magnet-based scratch method for standardisation of wound-healing assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718675/
https://www.ncbi.nlm.nih.gov/pubmed/31477739
http://dx.doi.org/10.1038/s41598-019-48930-7
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