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Operant Assessment of DMTP Spatial Working Memory in Mice
Working memory (WM) is required to bridge the time between the moment of sensory perception and the usage of the acquired information for subsequent actions. Its frequent and pharmacoresistent impairment in mental health disorders urges the development of rodent paradigms through back-translation of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718719/ https://www.ncbi.nlm.nih.gov/pubmed/31507388 http://dx.doi.org/10.3389/fnbeh.2019.00193 |
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author | Teutsch, Jasper Kätzel, Dennis |
author_facet | Teutsch, Jasper Kätzel, Dennis |
author_sort | Teutsch, Jasper |
collection | PubMed |
description | Working memory (WM) is required to bridge the time between the moment of sensory perception and the usage of the acquired information for subsequent actions. Its frequent and pharmacoresistent impairment in mental health disorders urges the development of rodent paradigms through back-translation of human WM tests, ideally avoiding the confounds of alternation-based assays. Here we show, that mice can acquire a delayed-matching-to-position (DMTP) operant spatial WM (SWM) paradigm that is akin to the combined attention and memory (CAM) task previously developed for rats, and that relies on a 5-choice wall [5-CSWM, 5-choice based operant testing of SWM (5-CSWM)]. Requiring ca. 3 months of daily training with a non-illuminated operant box in the default state, mice could attain a performance level of ≥70% choice accuracy with short (2 s) delays in the DMTP 5-CSWM task. Performance decreased with extended delays, as expected for WM processes. Modafinil (15 and 30 mg/kg) and guanfacine (0.3 and 1 mg/kg) showed no consistent efficacy in enhancing task performance. We also found, that mice did not improve beyond chance level, when trained in the DNMTP-version of the 5-CSWM. Our results outline the methodical possibility and constraints of assessing spatial WM in mice with an operant paradigm that provides high control over potentially confounding variables, such as cue-directed attention, motivation or mediating strategies like body-positioning. |
format | Online Article Text |
id | pubmed-6718719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67187192019-09-10 Operant Assessment of DMTP Spatial Working Memory in Mice Teutsch, Jasper Kätzel, Dennis Front Behav Neurosci Behavioral Neuroscience Working memory (WM) is required to bridge the time between the moment of sensory perception and the usage of the acquired information for subsequent actions. Its frequent and pharmacoresistent impairment in mental health disorders urges the development of rodent paradigms through back-translation of human WM tests, ideally avoiding the confounds of alternation-based assays. Here we show, that mice can acquire a delayed-matching-to-position (DMTP) operant spatial WM (SWM) paradigm that is akin to the combined attention and memory (CAM) task previously developed for rats, and that relies on a 5-choice wall [5-CSWM, 5-choice based operant testing of SWM (5-CSWM)]. Requiring ca. 3 months of daily training with a non-illuminated operant box in the default state, mice could attain a performance level of ≥70% choice accuracy with short (2 s) delays in the DMTP 5-CSWM task. Performance decreased with extended delays, as expected for WM processes. Modafinil (15 and 30 mg/kg) and guanfacine (0.3 and 1 mg/kg) showed no consistent efficacy in enhancing task performance. We also found, that mice did not improve beyond chance level, when trained in the DNMTP-version of the 5-CSWM. Our results outline the methodical possibility and constraints of assessing spatial WM in mice with an operant paradigm that provides high control over potentially confounding variables, such as cue-directed attention, motivation or mediating strategies like body-positioning. Frontiers Media S.A. 2019-08-27 /pmc/articles/PMC6718719/ /pubmed/31507388 http://dx.doi.org/10.3389/fnbeh.2019.00193 Text en Copyright © 2019 Teutsch and Kätzel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Behavioral Neuroscience Teutsch, Jasper Kätzel, Dennis Operant Assessment of DMTP Spatial Working Memory in Mice |
title | Operant Assessment of DMTP Spatial Working Memory in Mice |
title_full | Operant Assessment of DMTP Spatial Working Memory in Mice |
title_fullStr | Operant Assessment of DMTP Spatial Working Memory in Mice |
title_full_unstemmed | Operant Assessment of DMTP Spatial Working Memory in Mice |
title_short | Operant Assessment of DMTP Spatial Working Memory in Mice |
title_sort | operant assessment of dmtp spatial working memory in mice |
topic | Behavioral Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6718719/ https://www.ncbi.nlm.nih.gov/pubmed/31507388 http://dx.doi.org/10.3389/fnbeh.2019.00193 |
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