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Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods

The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers using central composite design. The ethanol concentration (33–67%), extraction temperature (33–67 °C), and extraction time (17–33 min) were analyzed as th...

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Autores principales: Savic Gajic, Ivana, Savic, Ivan, Boskov, Ivana, Žerajić, Stanko, Markovic, Ivana, Gajic, Dragoljub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719120/
https://www.ncbi.nlm.nih.gov/pubmed/31357622
http://dx.doi.org/10.3390/antiox8080248
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author Savic Gajic, Ivana
Savic, Ivan
Boskov, Ivana
Žerajić, Stanko
Markovic, Ivana
Gajic, Dragoljub
author_facet Savic Gajic, Ivana
Savic, Ivan
Boskov, Ivana
Žerajić, Stanko
Markovic, Ivana
Gajic, Dragoljub
author_sort Savic Gajic, Ivana
collection PubMed
description The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers using central composite design. The ethanol concentration (33–67%), extraction temperature (33–67 °C), and extraction time (17–33 min) were analyzed as the factors that impact the total phenolic content. The liquid-to-solid ratio of 10 cm(3) g(−1) was the same during extractions. The optimal conditions were found to be 59 °C, 60% (v/v) ethanol, and extraction time of 30 min. The total phenolic content (TPC = 3.12 g(GAE) 100 g(−1) dry plant material) and antioxidant activity (IC(50) = 120.5 µg cm(−3)) of the extract obtained by ultrasound-assisted extraction were compared with those obtained by maceration (TPC = 2.54 g(GAE) 100 g(−1) dry plant material, IC(50) = 150.6 µg cm(−3)) and Soxhlet extraction (TPC = 3.22 g(GAE) 100 g(−1) dry plant material, IC(50) = 204.2 µg cm(−3)). The ultrasound-assisted extraction gave higher total phenolic content and better antioxidant activity for shorter extraction time so that it represents the technique of choice for the extraction of phenolic compounds. The obtained extract, as the source of antioxidants, can be applied in the pharmaceutical and food industries.
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spelling pubmed-67191202019-09-10 Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods Savic Gajic, Ivana Savic, Ivan Boskov, Ivana Žerajić, Stanko Markovic, Ivana Gajic, Dragoljub Antioxidants (Basel) Article The aim of this study was to optimize the ultrasound-assisted extraction of phenolic compounds from black locust (Robiniae pseudoacaciae) flowers using central composite design. The ethanol concentration (33–67%), extraction temperature (33–67 °C), and extraction time (17–33 min) were analyzed as the factors that impact the total phenolic content. The liquid-to-solid ratio of 10 cm(3) g(−1) was the same during extractions. The optimal conditions were found to be 59 °C, 60% (v/v) ethanol, and extraction time of 30 min. The total phenolic content (TPC = 3.12 g(GAE) 100 g(−1) dry plant material) and antioxidant activity (IC(50) = 120.5 µg cm(−3)) of the extract obtained by ultrasound-assisted extraction were compared with those obtained by maceration (TPC = 2.54 g(GAE) 100 g(−1) dry plant material, IC(50) = 150.6 µg cm(−3)) and Soxhlet extraction (TPC = 3.22 g(GAE) 100 g(−1) dry plant material, IC(50) = 204.2 µg cm(−3)). The ultrasound-assisted extraction gave higher total phenolic content and better antioxidant activity for shorter extraction time so that it represents the technique of choice for the extraction of phenolic compounds. The obtained extract, as the source of antioxidants, can be applied in the pharmaceutical and food industries. MDPI 2019-07-27 /pmc/articles/PMC6719120/ /pubmed/31357622 http://dx.doi.org/10.3390/antiox8080248 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Savic Gajic, Ivana
Savic, Ivan
Boskov, Ivana
Žerajić, Stanko
Markovic, Ivana
Gajic, Dragoljub
Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title_full Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title_fullStr Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title_full_unstemmed Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title_short Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Locust (Robiniae Pseudoacaciae) Flowers and Comparison with Conventional Methods
title_sort optimization of ultrasound-assisted extraction of phenolic compounds from black locust (robiniae pseudoacaciae) flowers and comparison with conventional methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719120/
https://www.ncbi.nlm.nih.gov/pubmed/31357622
http://dx.doi.org/10.3390/antiox8080248
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