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Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development

Two mesenchymal zinc transporters, ZIP7 and ZIP13, play critical roles in dermal development. ZIP7 and ZIP13 are the closest among the conserved mammalian zinc transporters. However, whether their functions are complementary remains a controversial issue. In the present study, we found that the expr...

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Autores principales: Lee, Mi-Gi, Bin, Bum-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719138/
https://www.ncbi.nlm.nih.gov/pubmed/31412620
http://dx.doi.org/10.3390/ijms20163941
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author Lee, Mi-Gi
Bin, Bum-Ho
author_facet Lee, Mi-Gi
Bin, Bum-Ho
author_sort Lee, Mi-Gi
collection PubMed
description Two mesenchymal zinc transporters, ZIP7 and ZIP13, play critical roles in dermal development. ZIP7 and ZIP13 are the closest among the conserved mammalian zinc transporters. However, whether their functions are complementary remains a controversial issue. In the present study, we found that the expression of ZIP13, but not ZIP7, is elevated by transforming growth factor beta (TGF-β) treatment, indicating that TGF-β-mediated ZIP13 amplification is crucial for collagen production during dermal development. Genome-wide gene expression analysis revealed that ~26% of genes are dependent on either ZIP7 or ZIP13, which is greater than the ~17% of genes dependent on both of them. ZIP7 depletion induces endoplasmic reticulum (ER) stress in mesenchymal stem cells, resulting in significant inhibition of fibrogenic differentiation. However, ZIP13 depletion does not induce ER stress. Though both ZIP7 and ZIP13 contain traditional ER signal peptides for their intracellular localization, their distributions are distinct. When ZIP7 and ZIP13 are coexpressed, their localizations are distinct; ZIP7 is located on the ER, but ZIP13 is located on both the ER and Golgi, indicating that only ZIP13 is a zinc gatekeeper on the Golgi. Our data illustrate that the different actions of ZIP7 and ZIP13 are crucial for dermal development.
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spelling pubmed-67191382019-09-10 Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development Lee, Mi-Gi Bin, Bum-Ho Int J Mol Sci Article Two mesenchymal zinc transporters, ZIP7 and ZIP13, play critical roles in dermal development. ZIP7 and ZIP13 are the closest among the conserved mammalian zinc transporters. However, whether their functions are complementary remains a controversial issue. In the present study, we found that the expression of ZIP13, but not ZIP7, is elevated by transforming growth factor beta (TGF-β) treatment, indicating that TGF-β-mediated ZIP13 amplification is crucial for collagen production during dermal development. Genome-wide gene expression analysis revealed that ~26% of genes are dependent on either ZIP7 or ZIP13, which is greater than the ~17% of genes dependent on both of them. ZIP7 depletion induces endoplasmic reticulum (ER) stress in mesenchymal stem cells, resulting in significant inhibition of fibrogenic differentiation. However, ZIP13 depletion does not induce ER stress. Though both ZIP7 and ZIP13 contain traditional ER signal peptides for their intracellular localization, their distributions are distinct. When ZIP7 and ZIP13 are coexpressed, their localizations are distinct; ZIP7 is located on the ER, but ZIP13 is located on both the ER and Golgi, indicating that only ZIP13 is a zinc gatekeeper on the Golgi. Our data illustrate that the different actions of ZIP7 and ZIP13 are crucial for dermal development. MDPI 2019-08-13 /pmc/articles/PMC6719138/ /pubmed/31412620 http://dx.doi.org/10.3390/ijms20163941 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Mi-Gi
Bin, Bum-Ho
Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title_full Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title_fullStr Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title_full_unstemmed Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title_short Different Actions of Intracellular Zinc Transporters ZIP7 and ZIP13 Are Essential for Dermal Development
title_sort different actions of intracellular zinc transporters zip7 and zip13 are essential for dermal development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719138/
https://www.ncbi.nlm.nih.gov/pubmed/31412620
http://dx.doi.org/10.3390/ijms20163941
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