Influence of Traditional vs Alternative Dietary Carbohydrates Sources on the Large Intestinal Microbiota in Post-Weaning Piglets

SIMPLE SUMMARY: Nutritional and environmental changes result in significant physiological changes in pigs at the weaning stage. The post-weaning period is mainly characterized by low feed intake and feed efficiency, together with intestinal disturbances. Maximizing the energy intake is known to be critical for promoting growth in weaned piglets, and it is essential to formulate diets with highly digestible and absorbable nutrients/ingredients, as the degree of intestinal maturation is limited. The current challenge is to find new sustainable, effective, and simple carbohydrate sources to satisfy these conditions without producing detrimental effects on the gut ecosystem. In this research, processed and ready-to-eat food products that are no longer suitable for humans were tested, which have high potential as an alternative energy source for pig nutrition. The results demonstrated that replacing conventional ingredients with highly digestible and simple carbohydrate-rich ingredients in the diets of post-weaning piglets did not affect their growth. However, both the abundance and composition of the bacterial community in the large intestine changed. Thus, the results should be interpreted with caution, as they are case-specific, and when these alternative feed ingredients are used in the post-weaning period, their inclusion rate and their effect on microbiota must be carefully considered. ABSTRACT: In this study, common cereal grains were partially replaced by former foodstuffs products (FFPs) in post-weaning piglets’ diets, to investigate how these alternative ingredients influence the faecal microbiota in the post-weaning period. Twelve post-weaning piglets were housed for 16 days in individual pens and were then fed two diets: a standard wheat-barley-corn meal diet and a diet containing 30% FFPs, thus partially substituting conventional cereals. The growth performance was monitored and faecal microbiota was characterized by the next generation sequencing of the 16S rRNA gene. The results showed no detrimental effects on growth performance when FFPs were used. However, the FFP diet decreased the bacterial richness and evenness in the large intestine, while minor differences were observed in the taxa composition. The core microbiota composition was only slightly affected, and no differences between the two groups in the gut microbiota composition at the phylum level over time were observed. Thus, although these results should be interpreted with caution, as they are case-specific, FFPs can be potentially used as alternative carbohydrate sources in post-weaning piglets, but further investigations are necessary to clarify their impact on gut health when used for a longer period.