Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor

Lattice light-sheet microscopy (LLSM) is valuable for its combination of reduced photobleaching and outstanding spatiotemporal resolution in 3D. Using LLSM to image biosensors in living cells could provide unprecedented visualization of rapid, localized changes in protein conformation or posttransla...

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Detalles Bibliográficos
Autores principales: O’Shaughnessy, Ellen C., Stone, Orrin J., LaFosse, Paul K., Azoitei, Mihai L., Tsygankov, Denis, Heddleston, John M., Legant, Wesley R., Wittchen, Erika S., Burridge, Keith, Elston, Timothy C., Betzig, Eric, Chew, Teng-Leong, Adalsteinsson, David, Hahn, Klaus M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719445/
https://www.ncbi.nlm.nih.gov/pubmed/31444239
http://dx.doi.org/10.1083/jcb.201903019

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