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Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells

We have previously reported human fetal cartilage progenitor cells (hFCPCs) as a novel source of therapeutic cells showing high proliferation and stem cell properties superior to those of adult mesenchymal stem cells (MSCs). In this study, we investigated the immunophenotype and immune-modulatory ac...

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Autores principales: Lee, Su Jeong, Kim, Jiyoung, Choi, Woo Hee, Park, So Ra, Choi, Byung Hyune, Min, Byoung-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719489/
https://www.ncbi.nlm.nih.gov/pubmed/30983392
http://dx.doi.org/10.1177/0963689719842166
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author Lee, Su Jeong
Kim, Jiyoung
Choi, Woo Hee
Park, So Ra
Choi, Byung Hyune
Min, Byoung-Hyun
author_facet Lee, Su Jeong
Kim, Jiyoung
Choi, Woo Hee
Park, So Ra
Choi, Byung Hyune
Min, Byoung-Hyun
author_sort Lee, Su Jeong
collection PubMed
description We have previously reported human fetal cartilage progenitor cells (hFCPCs) as a novel source of therapeutic cells showing high proliferation and stem cell properties superior to those of adult mesenchymal stem cells (MSCs). In this study, we investigated the immunophenotype and immune-modulatory activities of hFCPCs. With institutional review board approval, hFCPCs were isolated from fetuses at 11–13 weeks of gestation. hFCPCs showed strong expression of HLA class I molecules but low or no expression of HLA class II and co-stimulatory molecules, which was not changed significantly after 4 days of IFN-γ treatment. In a mixed lymphocyte reaction (MLR), hFCPCs showed no allogeneic immune response to peripheral blood lymphocytes (PBLs) and suppressed concanavalin A (Con A)-mediated proliferation of PBLs in a dose-dependent manner. In addition, hFCPCs inhibited Con A-induced secretion of pro-inflammatory cytokines TNF-α and IFN-γ from PBLs but showed no significant decrease of secretion of IL-10, anti-inflammatory cytokine. Co-culture of hFCPCs with stimulated PBLs for 4 days resulted in a significant increase in CD4(+)CD25(+)FoxP3(+) T regulatory cells (Tregs). hFCPCs expressed LIF, TGF-β1, TSG-6, and sHLA-G5 but did not express IDO and HGF. Stimulation of hFCPCs with TNF-α for 12 h showed slight induction in the expression of LIF, TSG-6, IDO, and HGF, whereas stimulation with IFN-γ did not affect expression of any of these factors. These results suggest that hFCPCs have low allogeneic immunogenicity and immune-modulatory activity in vitro, comparable to those of MSCs. However, compared with MSCs, hFCPCs were less responsive to TNF-α and IFN-γ, and the mechanisms underlying responses to these two cell types appeared distinct.
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spelling pubmed-67194892019-09-12 Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells Lee, Su Jeong Kim, Jiyoung Choi, Woo Hee Park, So Ra Choi, Byung Hyune Min, Byoung-Hyun Cell Transplant Original Articles We have previously reported human fetal cartilage progenitor cells (hFCPCs) as a novel source of therapeutic cells showing high proliferation and stem cell properties superior to those of adult mesenchymal stem cells (MSCs). In this study, we investigated the immunophenotype and immune-modulatory activities of hFCPCs. With institutional review board approval, hFCPCs were isolated from fetuses at 11–13 weeks of gestation. hFCPCs showed strong expression of HLA class I molecules but low or no expression of HLA class II and co-stimulatory molecules, which was not changed significantly after 4 days of IFN-γ treatment. In a mixed lymphocyte reaction (MLR), hFCPCs showed no allogeneic immune response to peripheral blood lymphocytes (PBLs) and suppressed concanavalin A (Con A)-mediated proliferation of PBLs in a dose-dependent manner. In addition, hFCPCs inhibited Con A-induced secretion of pro-inflammatory cytokines TNF-α and IFN-γ from PBLs but showed no significant decrease of secretion of IL-10, anti-inflammatory cytokine. Co-culture of hFCPCs with stimulated PBLs for 4 days resulted in a significant increase in CD4(+)CD25(+)FoxP3(+) T regulatory cells (Tregs). hFCPCs expressed LIF, TGF-β1, TSG-6, and sHLA-G5 but did not express IDO and HGF. Stimulation of hFCPCs with TNF-α for 12 h showed slight induction in the expression of LIF, TSG-6, IDO, and HGF, whereas stimulation with IFN-γ did not affect expression of any of these factors. These results suggest that hFCPCs have low allogeneic immunogenicity and immune-modulatory activity in vitro, comparable to those of MSCs. However, compared with MSCs, hFCPCs were less responsive to TNF-α and IFN-γ, and the mechanisms underlying responses to these two cell types appeared distinct. SAGE Publications 2019-04-14 2019-07 /pmc/articles/PMC6719489/ /pubmed/30983392 http://dx.doi.org/10.1177/0963689719842166 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Lee, Su Jeong
Kim, Jiyoung
Choi, Woo Hee
Park, So Ra
Choi, Byung Hyune
Min, Byoung-Hyun
Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title_full Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title_fullStr Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title_full_unstemmed Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title_short Immunophenotype and Immune-Modulatory Activities of Human Fetal Cartilage-Derived Progenitor Cells
title_sort immunophenotype and immune-modulatory activities of human fetal cartilage-derived progenitor cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719489/
https://www.ncbi.nlm.nih.gov/pubmed/30983392
http://dx.doi.org/10.1177/0963689719842166
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