Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluor...

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Detalles Bibliográficos
Autores principales: Wu, Jiahui, Zaccara, Sara, Khuperkar, Deepak, Kim, Hyaeyeong, Tanenbaum, Marvin E., Jaffrey, Samie R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719798/
https://www.ncbi.nlm.nih.gov/pubmed/31471614
http://dx.doi.org/10.1038/s41592-019-0531-7
Descripción
Sumario:Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted in mRNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

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