Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluor...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jiahui, Zaccara, Sara, Khuperkar, Deepak, Kim, Hyaeyeong, Tanenbaum, Marvin E., Jaffrey, Samie R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719798/
https://www.ncbi.nlm.nih.gov/pubmed/31471614
http://dx.doi.org/10.1038/s41592-019-0531-7
_version_ 1783447987114999808
author Wu, Jiahui
Zaccara, Sara
Khuperkar, Deepak
Kim, Hyaeyeong
Tanenbaum, Marvin E.
Jaffrey, Samie R.
author_facet Wu, Jiahui
Zaccara, Sara
Khuperkar, Deepak
Kim, Hyaeyeong
Tanenbaum, Marvin E.
Jaffrey, Samie R.
author_sort Wu, Jiahui
collection PubMed
description Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted in mRNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.
format Online
Article
Text
id pubmed-6719798
institution National Center for Biotechnology Information
language English
publishDate 2019
record_format MEDLINE/PubMed
spelling pubmed-67197982020-02-29 Live imaging of mRNA using RNA-stabilized fluorogenic proteins Wu, Jiahui Zaccara, Sara Khuperkar, Deepak Kim, Hyaeyeong Tanenbaum, Marvin E. Jaffrey, Samie R. Nat Methods Article Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted in mRNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells. 2019-08-30 2019-09 /pmc/articles/PMC6719798/ /pubmed/31471614 http://dx.doi.org/10.1038/s41592-019-0531-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wu, Jiahui
Zaccara, Sara
Khuperkar, Deepak
Kim, Hyaeyeong
Tanenbaum, Marvin E.
Jaffrey, Samie R.
Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title_full Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title_fullStr Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title_full_unstemmed Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title_short Live imaging of mRNA using RNA-stabilized fluorogenic proteins
title_sort live imaging of mrna using rna-stabilized fluorogenic proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719798/
https://www.ncbi.nlm.nih.gov/pubmed/31471614
http://dx.doi.org/10.1038/s41592-019-0531-7
work_keys_str_mv AT wujiahui liveimagingofmrnausingrnastabilizedfluorogenicproteins
AT zaccarasara liveimagingofmrnausingrnastabilizedfluorogenicproteins
AT khuperkardeepak liveimagingofmrnausingrnastabilizedfluorogenicproteins
AT kimhyaeyeong liveimagingofmrnausingrnastabilizedfluorogenicproteins
AT tanenbaummarvine liveimagingofmrnausingrnastabilizedfluorogenicproteins
AT jaffreysamier liveimagingofmrnausingrnastabilizedfluorogenicproteins

Ejemplares similares