Cargando…

Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. Here we describe fluorogenic proteins whose fluorescence is activated by RNA aptamers. Fluor...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wu, Jiahui, Zaccara, Sara, Khuperkar, Deepak, Kim, Hyaeyeong, Tanenbaum, Marvin E., Jaffrey, Samie R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719798/ https://www.ncbi.nlm.nih.gov/pubmed/31471614 http://dx.doi.org/10.1038/s41592-019-0531-7

Ejemplares similares

mRNA ageing shapes the Cap2 methylome in mammalian mRNA
por: Despic, Vladimir, et al.
Publicado: (2023)

Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity in mRNA Decoding
por: Boersma, Sanne, et al.
Publicado: (2019)

m(6)A enhances the phase separation potential of mRNA
por: Ries, Ryan J., et al.
Publicado: (2019)

Expanding the epitranscriptome: Dihydrouridine in mRNA
por: Dixit, Sameer, et al.
Publicado: (2022)

Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated mRNA Decay
por: Hoek, Tim A., et al.
Publicado: (2019)

Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex
por: Song, Wenjiao, et al.
Publicado: (2017)

Emerging links between m(6)A and misregulated mRNA methylation in cancer
por: Jaffrey, Samie R., et al.
Publicado: (2017)

Co-crystal structures of the fluorogenic aptamer Beetroot show that close homology may not predict similar RNA architecture
por: Passalacqua, Luiz F. M., et al.
Publicado: (2023)

Alternative splicing of METTL3 explains apparently METTL3-independent m(6)A modifications in mRNA
por: Poh, Hui Xian, et al.
Publicado: (2022)

Regulation of mRNA translation during mitosis
por: Tanenbaum, Marvin E, et al.
Publicado: (2015)

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex
por: Jansens, Robert J.J., et al.
Publicado: (2022)

Imaging bacterial protein expression using genetically encoded sensors composed of RNA
por: Song, Wenjiao, et al.
Publicado: (2013)

Dynamics of Translation of Single mRNA Molecules In Vivo
por: Yan, Xiaowei, et al.
Publicado: (2016)

Live cell imaging of single RNA molecules with fluorogenic Mango II arrays
por: Cawte, Adam D., et al.
Publicado: (2020)

A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells
por: Bouhedda, Farah, et al.
Publicado: (2019)

Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs
por: Zheng, Ru, et al.
Publicado: (2023)

Genetically encoded RNA-based sensors with Pepper fluorogenic aptamer
por: Chen, Zhenyin, et al.
Publicado: (2023)

Synthetic biology tools to promote the folding and function of RNA aptamers in mammalian cells
por: Hou, Qian, et al.
Publicado: (2023)

Development of Highly Fluorogenic Styrene Probes for Visualizing RNA in Live Cells
por: Kim, Moon Jung, et al.
Publicado: (2023)

Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts
por: Litke, Jacob L., et al.
Publicado: (2019)

Time-resolved single-cell sequencing identifies multiple waves of mRNA decay during the mitosis-to-G1 phase transition
por: Krenning, Lenno, et al.
Publicado: (2022)

Stability of mRNA/DNA and DNA/DNA Duplexes Affects mRNA Transcription
por: Kraeva, Rayna I., et al.
Publicado: (2007)

Imaging Single-mRNA Localization and Translation in Live Neurons
por: Lee, Byung Hun, et al.
Publicado: (2016)

Geneticin reduces mRNA stability
por: Durmaz, Yavuz T., et al.
Publicado: (2022)

Nanobiotechnology-Enabled mRNA Stabilization
por: Xian, He, et al.
Publicado: (2023)

Modifications of mRNA vaccine structural elements for improving mRNA stability and translation efficiency
por: Kim, Sun Chang, et al.
Publicado: (2021)

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution
por: Filonov, Grigory S., et al.
Publicado: (2014)

The human 18S rRNA m(6)A methyltransferase METTL5 is stabilized by TRMT112
por: van Tran, Nhan, et al.
Publicado: (2019)

A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat RNA
por: Strack, Rita L., et al.
Publicado: (2013)

Tandem Spinach Array for mRNA Imaging in Living Bacterial Cells
por: Zhang, Jichuan, et al.
Publicado: (2015)

Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery
por: Baladi, Tom, et al.
Publicado: (2021)

Long-term imaging of individual mRNA molecules in living cells
por: Guo, Yue, et al.
Publicado: (2022)

The emerging role of RNA modifications in the regulation of mRNA stability
por: Boo, Sung Ho, et al.
Publicado: (2020)

Matrin 3 Binds and Stabilizes mRNA
por: Salton, Maayan, et al.
Publicado: (2011)

Cardiovascular disease, inflammation, and mRNA stability
por: Herman, Allison B., et al.
Publicado: (2018)

Research Advances on the Stability of mRNA Vaccines
por: Cheng, Feiran, et al.
Publicado: (2023)

Condition-specific 3′ mRNA isoform half-lives and stability elements in yeast
por: Geisberg, Joseph V., et al.
Publicado: (2023)

PEGylation of mRNA by Hybridization of Complementary PEG-RNA Oligonucleotides Stabilizes mRNA without Using Cationic Materials
por: Yoshinaga, Naoto, et al.
Publicado: (2021)

Visualization of mRNA translation in living cells
por: Rodriguez, Alexis J., et al.
Publicado: (2006)

mRNA analysis of single living cells
por: Osada, Toshiya, et al.
Publicado: (2003)

Cannot write session to /tmp/vufind_sessions/sess_t5mirj91krte6ftc98so5qceig