A Comprehensive Review of Non-Covalent Radiofluorination Approaches Using Aluminum [(18)F]fluoride: Will [(18)F]AlF Replace (68)Ga for Metal Chelate Labeling?

Due to its ideal physical properties, fluorine-18 turns out to be a key radionuclide for positron emission tomography (PET) imaging, for both preclinical and clinical applications. However, usual biomolecules radiofluorination procedures require the formation of covalent bonds with fluorinated prosthetic groups. This drawback makes radiofluorination impractical for routine radiolabeling, gallium-68 appearing to be much more convenient for the labeling of chelator-bearing PET probes. In response to this limitation, a recent expansion of the (18)F chemical toolbox gave aluminum [(18)F]fluoride chemistry a real prominence since the late 2000s. This approach is based on the formation of an [(18)F][AlF](2+) cation, complexed with a 9-membered cyclic chelator such as NOTA, NODA or their analogs. Allowing a one-step radiofluorination in an aqueous medium, this technique combines fluorine-18 and non-covalent radiolabeling with the advantage of being very easy to implement. Since its first reports, [(18)F]AlF radiolabeling approach has been applied to a wide variety of potential PET imaging vectors, whether of peptidic, proteic, or small molecule structure. Most of these [(18)F]AlF-labeled tracers showed promising preclinical results and have reached the clinical evaluation stage for some of them. The aim of this report is to provide a comprehensive overview of [(18)F]AlF labeling applications through a description of the various [(18)F]AlF-labeled conjugates, from their radiosynthesis to their evaluation as PET imaging agents.