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Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species
BACKGROUND: Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Previously, genotyping of Russian B.anthracis isolates was carried out using canSNP and MLVA data; these methods yield lower...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720099/ https://www.ncbi.nlm.nih.gov/pubmed/31477029 http://dx.doi.org/10.1186/s12864-019-6060-z |
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author | Pisarenko, Sergey V. Eremenko, Eugene I. Ryazanova, Alla G. Kovalev, Dmitry A. Buravtseva, Nina P. Aksenova, Lyudmila Yu. Dugarzhapova, Zorigma F. Evchenko, Anna Yu. Kravets, Elena V. Semenova, Olga V. Bobrisheva, Olga V. Kuznetsova, Irina V. Golovinskaya, Tatyana M. Volynkina, Anna S. Balakhonov, Sergei V. Kulichenko, Alexander N. |
author_facet | Pisarenko, Sergey V. Eremenko, Eugene I. Ryazanova, Alla G. Kovalev, Dmitry A. Buravtseva, Nina P. Aksenova, Lyudmila Yu. Dugarzhapova, Zorigma F. Evchenko, Anna Yu. Kravets, Elena V. Semenova, Olga V. Bobrisheva, Olga V. Kuznetsova, Irina V. Golovinskaya, Tatyana M. Volynkina, Anna S. Balakhonov, Sergei V. Kulichenko, Alexander N. |
author_sort | Pisarenko, Sergey V. |
collection | PubMed |
description | BACKGROUND: Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Previously, genotyping of Russian B.anthracis isolates was carried out using canSNP and MLVA data; these methods yield lower resolution results compared to whole genome SNP analysis (wgSNP). In this research, we have used wgSNP method for genotyping of 10 B.anthracis isolates, obtained during 1961–2016 in Russia on territory of Western Siberia. RESULTS: We have analyzed 185 B.anthracis genomes available in GenBank database and genomes of 10 isolates obtained in this study to determine the place of Russian isolates in the global phylogeny of B.anthracis. For the studied genomes we have detected 7203 SNPs, which were used for building a phylogenetic reconstruction with Maximum Likelihood Method. Results of the phylogenetic analysis indicate that Russian strains belong to three different genetic groups. Three strains belong to genetic group “Ames”, two strains – to “STI” group. Five strains belong to the main genetic line B, and four of them form a subcluster, described for the first time, which we have named “Siberia”. CONCLUSIONS: In this study, the data on genetic diversity of B.anthracis strains on the territory of Western Siberia is presented for the first time. As a result of complex phylogenetic analysis, the place of these isolates was determined in the global phylogenetic structure of the B.anthracis population. We describe a new cluster in the main genetic line B for the first time. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-6060-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6720099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67200992019-09-06 Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species Pisarenko, Sergey V. Eremenko, Eugene I. Ryazanova, Alla G. Kovalev, Dmitry A. Buravtseva, Nina P. Aksenova, Lyudmila Yu. Dugarzhapova, Zorigma F. Evchenko, Anna Yu. Kravets, Elena V. Semenova, Olga V. Bobrisheva, Olga V. Kuznetsova, Irina V. Golovinskaya, Tatyana M. Volynkina, Anna S. Balakhonov, Sergei V. Kulichenko, Alexander N. BMC Genomics Research Article BACKGROUND: Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Previously, genotyping of Russian B.anthracis isolates was carried out using canSNP and MLVA data; these methods yield lower resolution results compared to whole genome SNP analysis (wgSNP). In this research, we have used wgSNP method for genotyping of 10 B.anthracis isolates, obtained during 1961–2016 in Russia on territory of Western Siberia. RESULTS: We have analyzed 185 B.anthracis genomes available in GenBank database and genomes of 10 isolates obtained in this study to determine the place of Russian isolates in the global phylogeny of B.anthracis. For the studied genomes we have detected 7203 SNPs, which were used for building a phylogenetic reconstruction with Maximum Likelihood Method. Results of the phylogenetic analysis indicate that Russian strains belong to three different genetic groups. Three strains belong to genetic group “Ames”, two strains – to “STI” group. Five strains belong to the main genetic line B, and four of them form a subcluster, described for the first time, which we have named “Siberia”. CONCLUSIONS: In this study, the data on genetic diversity of B.anthracis strains on the territory of Western Siberia is presented for the first time. As a result of complex phylogenetic analysis, the place of these isolates was determined in the global phylogenetic structure of the B.anthracis population. We describe a new cluster in the main genetic line B for the first time. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-019-6060-z) contains supplementary material, which is available to authorized users. BioMed Central 2019-09-02 /pmc/articles/PMC6720099/ /pubmed/31477029 http://dx.doi.org/10.1186/s12864-019-6060-z Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Pisarenko, Sergey V. Eremenko, Eugene I. Ryazanova, Alla G. Kovalev, Dmitry A. Buravtseva, Nina P. Aksenova, Lyudmila Yu. Dugarzhapova, Zorigma F. Evchenko, Anna Yu. Kravets, Elena V. Semenova, Olga V. Bobrisheva, Olga V. Kuznetsova, Irina V. Golovinskaya, Tatyana M. Volynkina, Anna S. Balakhonov, Sergei V. Kulichenko, Alexander N. Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title | Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title_full | Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title_fullStr | Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title_full_unstemmed | Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title_short | Phylogenetic analysis of Bacillus anthracis strains from Western Siberia reveals a new genetic cluster in the global population of the species |
title_sort | phylogenetic analysis of bacillus anthracis strains from western siberia reveals a new genetic cluster in the global population of the species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720099/ https://www.ncbi.nlm.nih.gov/pubmed/31477029 http://dx.doi.org/10.1186/s12864-019-6060-z |
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