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Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products

Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene s...

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Autores principales: Sun, Xiang, Cai, You-Sheng, Yuan, Yujie, Bian, Guangkai, Ye, Ziling, Deng, Zixin, Liu, Tiangang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720227/
https://www.ncbi.nlm.nih.gov/pubmed/31501673
http://dx.doi.org/10.3762/bjoc.15.202
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author Sun, Xiang
Cai, You-Sheng
Yuan, Yujie
Bian, Guangkai
Ye, Ziling
Deng, Zixin
Liu, Tiangang
author_facet Sun, Xiang
Cai, You-Sheng
Yuan, Yujie
Bian, Guangkai
Ye, Ziling
Deng, Zixin
Liu, Tiangang
author_sort Sun, Xiang
collection PubMed
description Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene synthase from T. viride by genome mining and determined the structure of its corresponding products. One new 5/6 bicyclic sesquiterpene and its esterified derivative were characterised by GC–MS and 1D and 2D NMR spectroscopy. To the best of our knowledge, this is the first well-identified sesquiterpene synthase from T. viride to date.
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spelling pubmed-67202272019-09-09 Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products Sun, Xiang Cai, You-Sheng Yuan, Yujie Bian, Guangkai Ye, Ziling Deng, Zixin Liu, Tiangang Beilstein J Org Chem Full Research Paper Sesquiterpene synthases in Trichoderma viride have been seldom studied, despite the efficiency of filamentous fungi for terpenoid production. Using the farnesyl diphosphate-overexpressing Saccharomyces cerevisiae platform to produce diverse terpenoids, we herein identified an unknown sesquiterpene synthase from T. viride by genome mining and determined the structure of its corresponding products. One new 5/6 bicyclic sesquiterpene and its esterified derivative were characterised by GC–MS and 1D and 2D NMR spectroscopy. To the best of our knowledge, this is the first well-identified sesquiterpene synthase from T. viride to date. Beilstein-Institut 2019-08-28 /pmc/articles/PMC6720227/ /pubmed/31501673 http://dx.doi.org/10.3762/bjoc.15.202 Text en Copyright © 2019, Sun et al. https://creativecommons.org/licenses/by/4.0https://www.beilstein-journals.org/bjoc/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0). Please note that the reuse, redistribution and reproduction in particular requires that the authors and source are credited. The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc/terms)
spellingShingle Full Research Paper
Sun, Xiang
Cai, You-Sheng
Yuan, Yujie
Bian, Guangkai
Ye, Ziling
Deng, Zixin
Liu, Tiangang
Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title_full Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title_fullStr Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title_full_unstemmed Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title_short Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
title_sort genome mining in trichoderma viride j1-030: discovery and identification of novel sesquiterpene synthase and its products
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720227/
https://www.ncbi.nlm.nih.gov/pubmed/31501673
http://dx.doi.org/10.3762/bjoc.15.202
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