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Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements

Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many of them generate contradictory and non-compliant results. In our study, we focused on the characterization of traditionally used biochemical (2,2′-azino-bis-(3-ethylbenzothiazoline-6...

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Autores principales: Viktorova, Jitka, Stranska-Zachariasova, Milena, Fenclova, Marie, Vitek, Libor, Hajslova, Jana, Kren, Vladimir, Ruml, Tomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720444/
https://www.ncbi.nlm.nih.gov/pubmed/31426591
http://dx.doi.org/10.3390/antiox8080317
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author Viktorova, Jitka
Stranska-Zachariasova, Milena
Fenclova, Marie
Vitek, Libor
Hajslova, Jana
Kren, Vladimir
Ruml, Tomas
author_facet Viktorova, Jitka
Stranska-Zachariasova, Milena
Fenclova, Marie
Vitek, Libor
Hajslova, Jana
Kren, Vladimir
Ruml, Tomas
author_sort Viktorova, Jitka
collection PubMed
description Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many of them generate contradictory and non-compliant results. In our study, we focused on the characterization of traditionally used biochemical (2,2′-azino-bis-(3-ethylbenzothiazoline-6 sulfonic acid) (ABTS), Oxygen Radical Absorption Capacity (ORAC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and cellular (CAA) antioxidant tests on a broad set of milk thistle dietary supplements containing silymarin. In addition to 26 commercially available preparations, also the natural silymarin extract available from Sigma Aldrich, St. Louis, MI, USA, and a model mixture of pure flavonoid/flavonolignans mimicking the silymarin composition were investigated as control samples. Significant differences in the antioxidant capacity of the supplements were observed. Unlike the DPPH, the results of the ABTS and ORAC methods correlated with the silymarin components determined by U-HPLC-HRMS/MS. The responses in CAA were considerably lower than in other assays. Silymarin exhibited a significantly higher antioxidant capacity than the artificially prepared flavonoid/flavonolignans mixture in all tests, indicating possible presence of other antioxidants of natural origin. The follow-up U-HPLC-HRMS/MS screening revealed the presence of tens of non-silymarin compounds with reported antioxidant activity (not only in the silymarin extract, but also in the milk thistle preparations). The sum of the total phenolics and the sum of the simple phenolics correlated with CAA results more than silymarin.
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spelling pubmed-67204442019-09-10 Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements Viktorova, Jitka Stranska-Zachariasova, Milena Fenclova, Marie Vitek, Libor Hajslova, Jana Kren, Vladimir Ruml, Tomas Antioxidants (Basel) Article Numerous in vitro assays are used to characterize the antioxidant properties of natural-based matrices. However, many of them generate contradictory and non-compliant results. In our study, we focused on the characterization of traditionally used biochemical (2,2′-azino-bis-(3-ethylbenzothiazoline-6 sulfonic acid) (ABTS), Oxygen Radical Absorption Capacity (ORAC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and cellular (CAA) antioxidant tests on a broad set of milk thistle dietary supplements containing silymarin. In addition to 26 commercially available preparations, also the natural silymarin extract available from Sigma Aldrich, St. Louis, MI, USA, and a model mixture of pure flavonoid/flavonolignans mimicking the silymarin composition were investigated as control samples. Significant differences in the antioxidant capacity of the supplements were observed. Unlike the DPPH, the results of the ABTS and ORAC methods correlated with the silymarin components determined by U-HPLC-HRMS/MS. The responses in CAA were considerably lower than in other assays. Silymarin exhibited a significantly higher antioxidant capacity than the artificially prepared flavonoid/flavonolignans mixture in all tests, indicating possible presence of other antioxidants of natural origin. The follow-up U-HPLC-HRMS/MS screening revealed the presence of tens of non-silymarin compounds with reported antioxidant activity (not only in the silymarin extract, but also in the milk thistle preparations). The sum of the total phenolics and the sum of the simple phenolics correlated with CAA results more than silymarin. MDPI 2019-08-18 /pmc/articles/PMC6720444/ /pubmed/31426591 http://dx.doi.org/10.3390/antiox8080317 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Viktorova, Jitka
Stranska-Zachariasova, Milena
Fenclova, Marie
Vitek, Libor
Hajslova, Jana
Kren, Vladimir
Ruml, Tomas
Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title_full Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title_fullStr Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title_full_unstemmed Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title_short Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements
title_sort complex evaluation of antioxidant capacity of milk thistle dietary supplements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720444/
https://www.ncbi.nlm.nih.gov/pubmed/31426591
http://dx.doi.org/10.3390/antiox8080317
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