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Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1

Little information is available regarding the effect of melatonin on the quality and fertilization capability of sex-sorted bull sperm, and even less about the associated mechanism. Sex-sorted sperm from three individual bulls were washed twice in wash medium and incubated in a fertilization medium...

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Autores principales: Li, Chong-Yang, Hao, Hai-Sheng, Zhao, Ya-Han, Zhang, Pei-Pei, Wang, Hao-Yu, Pang, Yun-Wei, Du, Wei-Hua, Zhao, Shan-Jiang, Liu, Yan, Huang, Jin-Ming, Wang, Jing-Jing, Ruan, Wei-Min, Hao, Tong, Reiter, Russel J., Zhu, Hua-Bin, Zhao, Xue-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720564/
https://www.ncbi.nlm.nih.gov/pubmed/31409031
http://dx.doi.org/10.3390/ijms20163921
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author Li, Chong-Yang
Hao, Hai-Sheng
Zhao, Ya-Han
Zhang, Pei-Pei
Wang, Hao-Yu
Pang, Yun-Wei
Du, Wei-Hua
Zhao, Shan-Jiang
Liu, Yan
Huang, Jin-Ming
Wang, Jing-Jing
Ruan, Wei-Min
Hao, Tong
Reiter, Russel J.
Zhu, Hua-Bin
Zhao, Xue-Ming
author_facet Li, Chong-Yang
Hao, Hai-Sheng
Zhao, Ya-Han
Zhang, Pei-Pei
Wang, Hao-Yu
Pang, Yun-Wei
Du, Wei-Hua
Zhao, Shan-Jiang
Liu, Yan
Huang, Jin-Ming
Wang, Jing-Jing
Ruan, Wei-Min
Hao, Tong
Reiter, Russel J.
Zhu, Hua-Bin
Zhao, Xue-Ming
author_sort Li, Chong-Yang
collection PubMed
description Little information is available regarding the effect of melatonin on the quality and fertilization capability of sex-sorted bull sperm, and even less about the associated mechanism. Sex-sorted sperm from three individual bulls were washed twice in wash medium and incubated in a fertilization medium for 1.5 h, and each was supplemented with melatonin (0, 10(−3) M, 10(−5) M, 10(−7) M, and 10(−9) M). The reactive oxygen species (ROS) and endogenous antioxidant activity (glutathione peroxidase (GPx); superoxide dismutase (SOD); catalase (CAT)), apoptosis (phosphatidylserine [PS] externalization; mitochondrial membrane potential (Δψm)), acrosomal integrity events (malondialdehyde (MDA) level; acrosomal integrity), capacitation (calcium ion [Ca(2+)](i) level; cyclic adenosine monophosphate (cAMP); capacitation level), and fertilization ability of the sperm were assessed. Melatonin receptor 1 (MT1) and 2 (MT2) expression were examined to investigate the involvement of melatonin receptors on sex-sorted bull sperm capacitation. Our results show that treatment with 10(−5) M melatonin significantly decreased the ROS level and increased the GPx, SOD, and CAT activities of sex-sorted bull sperm, which inhibited PS externalization and MDA levels, and improved Δψm, acrosomal integrity, and fertilization ability. Further experiments showed that melatonin regulates sperm capacitation via MT1. These findings contribute to improving the fertilization capacity of sex-sorted bull sperm and exploring the associated mechanism.
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spelling pubmed-67205642019-09-10 Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1 Li, Chong-Yang Hao, Hai-Sheng Zhao, Ya-Han Zhang, Pei-Pei Wang, Hao-Yu Pang, Yun-Wei Du, Wei-Hua Zhao, Shan-Jiang Liu, Yan Huang, Jin-Ming Wang, Jing-Jing Ruan, Wei-Min Hao, Tong Reiter, Russel J. Zhu, Hua-Bin Zhao, Xue-Ming Int J Mol Sci Article Little information is available regarding the effect of melatonin on the quality and fertilization capability of sex-sorted bull sperm, and even less about the associated mechanism. Sex-sorted sperm from three individual bulls were washed twice in wash medium and incubated in a fertilization medium for 1.5 h, and each was supplemented with melatonin (0, 10(−3) M, 10(−5) M, 10(−7) M, and 10(−9) M). The reactive oxygen species (ROS) and endogenous antioxidant activity (glutathione peroxidase (GPx); superoxide dismutase (SOD); catalase (CAT)), apoptosis (phosphatidylserine [PS] externalization; mitochondrial membrane potential (Δψm)), acrosomal integrity events (malondialdehyde (MDA) level; acrosomal integrity), capacitation (calcium ion [Ca(2+)](i) level; cyclic adenosine monophosphate (cAMP); capacitation level), and fertilization ability of the sperm were assessed. Melatonin receptor 1 (MT1) and 2 (MT2) expression were examined to investigate the involvement of melatonin receptors on sex-sorted bull sperm capacitation. Our results show that treatment with 10(−5) M melatonin significantly decreased the ROS level and increased the GPx, SOD, and CAT activities of sex-sorted bull sperm, which inhibited PS externalization and MDA levels, and improved Δψm, acrosomal integrity, and fertilization ability. Further experiments showed that melatonin regulates sperm capacitation via MT1. These findings contribute to improving the fertilization capacity of sex-sorted bull sperm and exploring the associated mechanism. MDPI 2019-08-12 /pmc/articles/PMC6720564/ /pubmed/31409031 http://dx.doi.org/10.3390/ijms20163921 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Chong-Yang
Hao, Hai-Sheng
Zhao, Ya-Han
Zhang, Pei-Pei
Wang, Hao-Yu
Pang, Yun-Wei
Du, Wei-Hua
Zhao, Shan-Jiang
Liu, Yan
Huang, Jin-Ming
Wang, Jing-Jing
Ruan, Wei-Min
Hao, Tong
Reiter, Russel J.
Zhu, Hua-Bin
Zhao, Xue-Ming
Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title_full Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title_fullStr Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title_full_unstemmed Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title_short Melatonin Improves the Fertilization Capacity of Sex-Sorted Bull Sperm by Inhibiting Apoptosis and Increasing Fertilization Capacitation via MT1
title_sort melatonin improves the fertilization capacity of sex-sorted bull sperm by inhibiting apoptosis and increasing fertilization capacitation via mt1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720564/
https://www.ncbi.nlm.nih.gov/pubmed/31409031
http://dx.doi.org/10.3390/ijms20163921
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