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H(2)O(2)-Based Method for Rapid Detection of Transgene-Free Rice Plants from Segregating CRISPR/Cas9 Genome-Edited Progenies

Genome-editing techniques such as CRISPR/Cas9 have been widely used in crop functional genomics and improvement. To efficiently deliver the guide RNA and Cas9, most studies still rely on Agrobacterium-mediated transformation, which involves a selection marker gene. However, several limiting factors...

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Detalles Bibliográficos
Autores principales: Wu, Tsung-Meng, Huang, Jian-Zhi, Oung, Hui-Min, Hsu, Yi-Ting, Tsai, Yu-Chang, Hong, Chwan-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720670/
https://www.ncbi.nlm.nih.gov/pubmed/31404948
http://dx.doi.org/10.3390/ijms20163885