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A New Approach for Spontaneous Silver Ions Immobilization onto Casein

The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, the influence of light irradiation on the process was shown. In order to investigate the mechanism of metal ions sorption by casein the zero, pseudo-first o...

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Autores principales: Pryshchepa, Oleksandra, Sagandykova, Gulyaim N., Pomastowski, Paweł, Railean-Plugaru, Viorica, Król, Anna, Rogowska, Agnieszka, Rodzik, Agnieszka, Sprynskyy, Myroslav, Buszewski, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720684/
https://www.ncbi.nlm.nih.gov/pubmed/31398861
http://dx.doi.org/10.3390/ijms20163864
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author Pryshchepa, Oleksandra
Sagandykova, Gulyaim N.
Pomastowski, Paweł
Railean-Plugaru, Viorica
Król, Anna
Rogowska, Agnieszka
Rodzik, Agnieszka
Sprynskyy, Myroslav
Buszewski, Bogusław
author_facet Pryshchepa, Oleksandra
Sagandykova, Gulyaim N.
Pomastowski, Paweł
Railean-Plugaru, Viorica
Król, Anna
Rogowska, Agnieszka
Rodzik, Agnieszka
Sprynskyy, Myroslav
Buszewski, Bogusław
author_sort Pryshchepa, Oleksandra
collection PubMed
description The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, the influence of light irradiation on the process was shown. In order to investigate the mechanism of metal ions sorption by casein the zero, pseudo-first order kinetics and Weber-Morris intra-particle diffusion as well as Langmuir and Freundlich isotherm models were used. Furthermore, to specify more precisely, the possible binding mechanism, the spectroscopic (FT-IR—Fourier Transform Infrared Spectroscopy, Raman), spectrometric (MALDI-TOF MS—Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry), microscopic (SEM—Scanning Electron Microscope, TEM/EDX—Transmission Electron Microscopy/Energy Dispersive X-ray detector) and thermal (TGA—Thermogravimetric Analysis, DTG—Derivative Thermogravimetry) analysis were performed. Kinetic study indicates that silver binding onto casein is a heterogeneous process with two main stages: initial rapid stage related to surface adsorption onto casein with immediate creation of silver nanoparticles and slower second stage of intraglobular diffusion with silver binding in chelated form (metalloproteins) or ion-exchange form. Spectroscopic techniques confirmed the binding process and MALDI-TOF MS analysis show the dominant contribution of the α-casein in the process. Moreover, the treatment of silver-casein complex by artificial physiological fluids was performed.
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spelling pubmed-67206842019-09-10 A New Approach for Spontaneous Silver Ions Immobilization onto Casein Pryshchepa, Oleksandra Sagandykova, Gulyaim N. Pomastowski, Paweł Railean-Plugaru, Viorica Król, Anna Rogowska, Agnieszka Rodzik, Agnieszka Sprynskyy, Myroslav Buszewski, Bogusław Int J Mol Sci Article The work presents the kinetic and isotherm studies of silver binding on casein, which was carried out using batch sorption technique. Moreover, the influence of light irradiation on the process was shown. In order to investigate the mechanism of metal ions sorption by casein the zero, pseudo-first order kinetics and Weber-Morris intra-particle diffusion as well as Langmuir and Freundlich isotherm models were used. Furthermore, to specify more precisely, the possible binding mechanism, the spectroscopic (FT-IR—Fourier Transform Infrared Spectroscopy, Raman), spectrometric (MALDI-TOF MS—Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry), microscopic (SEM—Scanning Electron Microscope, TEM/EDX—Transmission Electron Microscopy/Energy Dispersive X-ray detector) and thermal (TGA—Thermogravimetric Analysis, DTG—Derivative Thermogravimetry) analysis were performed. Kinetic study indicates that silver binding onto casein is a heterogeneous process with two main stages: initial rapid stage related to surface adsorption onto casein with immediate creation of silver nanoparticles and slower second stage of intraglobular diffusion with silver binding in chelated form (metalloproteins) or ion-exchange form. Spectroscopic techniques confirmed the binding process and MALDI-TOF MS analysis show the dominant contribution of the α-casein in the process. Moreover, the treatment of silver-casein complex by artificial physiological fluids was performed. MDPI 2019-08-08 /pmc/articles/PMC6720684/ /pubmed/31398861 http://dx.doi.org/10.3390/ijms20163864 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pryshchepa, Oleksandra
Sagandykova, Gulyaim N.
Pomastowski, Paweł
Railean-Plugaru, Viorica
Król, Anna
Rogowska, Agnieszka
Rodzik, Agnieszka
Sprynskyy, Myroslav
Buszewski, Bogusław
A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title_full A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title_fullStr A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title_full_unstemmed A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title_short A New Approach for Spontaneous Silver Ions Immobilization onto Casein
title_sort new approach for spontaneous silver ions immobilization onto casein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720684/
https://www.ncbi.nlm.nih.gov/pubmed/31398861
http://dx.doi.org/10.3390/ijms20163864
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