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In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inf...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720688/ https://www.ncbi.nlm.nih.gov/pubmed/31443178 http://dx.doi.org/10.3390/ma12162596 |
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author | Kotroni, Eleftheria Simirioti, Eleftheria Kikionis, Stefanos Sfiniadakis, Ioannis Siamidi, Aggeliki Karalis, Vangelis Vitsos, Andreas Vlachou, Marilena Ioannou, Efstathia Roussis, Vassilios Rallis, Michail |
author_facet | Kotroni, Eleftheria Simirioti, Eleftheria Kikionis, Stefanos Sfiniadakis, Ioannis Siamidi, Aggeliki Karalis, Vangelis Vitsos, Andreas Vlachou, Marilena Ioannou, Efstathia Roussis, Vassilios Rallis, Michail |
author_sort | Kotroni, Eleftheria |
collection | PubMed |
description | Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inflammatory activity of electrospun alginate micro/nanofibrous dressings loaded with the aqueous extract of Pinus halepensis bark (PHBE) was evaluated in vivo in mice. The upper back skin of SKH-1 female hairless mice was exposed to a single dose of ultraviolet radiation (3 MEDs) and the inflamed area was treated daily by the direct application of a nanofibrous patch. The condition of the skin was evaluated primarily on the basis of clinical observation, photo-documentation and histopathological assessment, while measurements of the erythema, hydration, transepidermal water loss (TEWL) and sebum production were also taken into account. The results showed that the topical application of alginate micro/nanofibrous dressings loaded with PHBE on UV-inflamed skin significantly attenuated inflammation damage, reducing the healing period. Increase of the loading dose of PHBE resulted in a proportional reduction of the extent, the density and the depth of skin inflammation. With the steadily increasing interest of the skin dressing industry towards nanofibrous matrices, electrospun nonwovens could serve as ideal candidates for the development of multifunctional anti-inflammatory care systems. |
format | Online Article Text |
id | pubmed-6720688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67206882019-09-10 In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin Kotroni, Eleftheria Simirioti, Eleftheria Kikionis, Stefanos Sfiniadakis, Ioannis Siamidi, Aggeliki Karalis, Vangelis Vitsos, Andreas Vlachou, Marilena Ioannou, Efstathia Roussis, Vassilios Rallis, Michail Materials (Basel) Article Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inflammatory activity of electrospun alginate micro/nanofibrous dressings loaded with the aqueous extract of Pinus halepensis bark (PHBE) was evaluated in vivo in mice. The upper back skin of SKH-1 female hairless mice was exposed to a single dose of ultraviolet radiation (3 MEDs) and the inflamed area was treated daily by the direct application of a nanofibrous patch. The condition of the skin was evaluated primarily on the basis of clinical observation, photo-documentation and histopathological assessment, while measurements of the erythema, hydration, transepidermal water loss (TEWL) and sebum production were also taken into account. The results showed that the topical application of alginate micro/nanofibrous dressings loaded with PHBE on UV-inflamed skin significantly attenuated inflammation damage, reducing the healing period. Increase of the loading dose of PHBE resulted in a proportional reduction of the extent, the density and the depth of skin inflammation. With the steadily increasing interest of the skin dressing industry towards nanofibrous matrices, electrospun nonwovens could serve as ideal candidates for the development of multifunctional anti-inflammatory care systems. MDPI 2019-08-15 /pmc/articles/PMC6720688/ /pubmed/31443178 http://dx.doi.org/10.3390/ma12162596 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kotroni, Eleftheria Simirioti, Eleftheria Kikionis, Stefanos Sfiniadakis, Ioannis Siamidi, Aggeliki Karalis, Vangelis Vitsos, Andreas Vlachou, Marilena Ioannou, Efstathia Roussis, Vassilios Rallis, Michail In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title | In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title_full | In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title_fullStr | In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title_full_unstemmed | In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title_short | In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin |
title_sort | in vivo evaluation of the anti-inflammatory activity of electrospun micro/nanofibrous patches loaded with pinus halepensis bark extract on hairless mice skin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720688/ https://www.ncbi.nlm.nih.gov/pubmed/31443178 http://dx.doi.org/10.3390/ma12162596 |
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