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In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin

Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inf...

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Autores principales: Kotroni, Eleftheria, Simirioti, Eleftheria, Kikionis, Stefanos, Sfiniadakis, Ioannis, Siamidi, Aggeliki, Karalis, Vangelis, Vitsos, Andreas, Vlachou, Marilena, Ioannou, Efstathia, Roussis, Vassilios, Rallis, Michail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720688/
https://www.ncbi.nlm.nih.gov/pubmed/31443178
http://dx.doi.org/10.3390/ma12162596
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author Kotroni, Eleftheria
Simirioti, Eleftheria
Kikionis, Stefanos
Sfiniadakis, Ioannis
Siamidi, Aggeliki
Karalis, Vangelis
Vitsos, Andreas
Vlachou, Marilena
Ioannou, Efstathia
Roussis, Vassilios
Rallis, Michail
author_facet Kotroni, Eleftheria
Simirioti, Eleftheria
Kikionis, Stefanos
Sfiniadakis, Ioannis
Siamidi, Aggeliki
Karalis, Vangelis
Vitsos, Andreas
Vlachou, Marilena
Ioannou, Efstathia
Roussis, Vassilios
Rallis, Michail
author_sort Kotroni, Eleftheria
collection PubMed
description Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inflammatory activity of electrospun alginate micro/nanofibrous dressings loaded with the aqueous extract of Pinus halepensis bark (PHBE) was evaluated in vivo in mice. The upper back skin of SKH-1 female hairless mice was exposed to a single dose of ultraviolet radiation (3 MEDs) and the inflamed area was treated daily by the direct application of a nanofibrous patch. The condition of the skin was evaluated primarily on the basis of clinical observation, photo-documentation and histopathological assessment, while measurements of the erythema, hydration, transepidermal water loss (TEWL) and sebum production were also taken into account. The results showed that the topical application of alginate micro/nanofibrous dressings loaded with PHBE on UV-inflamed skin significantly attenuated inflammation damage, reducing the healing period. Increase of the loading dose of PHBE resulted in a proportional reduction of the extent, the density and the depth of skin inflammation. With the steadily increasing interest of the skin dressing industry towards nanofibrous matrices, electrospun nonwovens could serve as ideal candidates for the development of multifunctional anti-inflammatory care systems.
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spelling pubmed-67206882019-09-10 In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin Kotroni, Eleftheria Simirioti, Eleftheria Kikionis, Stefanos Sfiniadakis, Ioannis Siamidi, Aggeliki Karalis, Vangelis Vitsos, Andreas Vlachou, Marilena Ioannou, Efstathia Roussis, Vassilios Rallis, Michail Materials (Basel) Article Skin inflammation is the most common symptom in dermatological diseases. It is usually treated by topically applied products, such as creams, gels and lotions. Skin dressings offer a promising alternative as they are endowed with more controlled administration conditions. In this study, the anti-inflammatory activity of electrospun alginate micro/nanofibrous dressings loaded with the aqueous extract of Pinus halepensis bark (PHBE) was evaluated in vivo in mice. The upper back skin of SKH-1 female hairless mice was exposed to a single dose of ultraviolet radiation (3 MEDs) and the inflamed area was treated daily by the direct application of a nanofibrous patch. The condition of the skin was evaluated primarily on the basis of clinical observation, photo-documentation and histopathological assessment, while measurements of the erythema, hydration, transepidermal water loss (TEWL) and sebum production were also taken into account. The results showed that the topical application of alginate micro/nanofibrous dressings loaded with PHBE on UV-inflamed skin significantly attenuated inflammation damage, reducing the healing period. Increase of the loading dose of PHBE resulted in a proportional reduction of the extent, the density and the depth of skin inflammation. With the steadily increasing interest of the skin dressing industry towards nanofibrous matrices, electrospun nonwovens could serve as ideal candidates for the development of multifunctional anti-inflammatory care systems. MDPI 2019-08-15 /pmc/articles/PMC6720688/ /pubmed/31443178 http://dx.doi.org/10.3390/ma12162596 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kotroni, Eleftheria
Simirioti, Eleftheria
Kikionis, Stefanos
Sfiniadakis, Ioannis
Siamidi, Aggeliki
Karalis, Vangelis
Vitsos, Andreas
Vlachou, Marilena
Ioannou, Efstathia
Roussis, Vassilios
Rallis, Michail
In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title_full In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title_fullStr In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title_full_unstemmed In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title_short In Vivo Evaluation of the Anti-Inflammatory Activity of Electrospun Micro/Nanofibrous Patches Loaded with Pinus halepensis Bark Extract on Hairless Mice Skin
title_sort in vivo evaluation of the anti-inflammatory activity of electrospun micro/nanofibrous patches loaded with pinus halepensis bark extract on hairless mice skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720688/
https://www.ncbi.nlm.nih.gov/pubmed/31443178
http://dx.doi.org/10.3390/ma12162596
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