Analysis of 19 Minerals and Cortisol in Red Deer Hair in Two Different Areas of the Stelvio National Park: A Preliminary Study

SIMPLE SUMMARY: Minerals play an important role in animals’ health and nutritional status and are associated with cortisol, a hormone involved in several physiological processes and with a fundamental role in allostasis, the process of achieving homeostasis. The aim of the present study is to perform a preliminary investigation on the concentration of 19 minerals and cortisol in the hair of red deer coming from two different areas of the Stelvio National Park. Results showed a close association between hair minerals and cortisol concentrations and an effect of deer origin. Hair minerals and cortisol assessment is an easy, rapid, and low cost screening method and it is useful in wildlife management programs, in order to investigate wild animals’ health status, to perform environmental studies, to assess the presence of contaminants in wild species and to determine risks for humans. In addition, it could be helpful in public health programs to estimate contamination risks linked to wild animal meat consumption or to assess the presence of contaminants in food-producing animals. ABSTRACT: The aim of the study was to perform an investigation on the concentration of 19 minerals and cortisol in red deer (Cervus elaphus) hair, a matrix that is easy to collect with non-invasive and painless sampling, able to represent an integrative values of long-term substance concentrations, and able to give useful information, also when performed on dead animals, given its extreme stability over time. In the study thirty-five animals were included, coming from two different sides of a valley in the Stelvio National Park, where official water analysis had pointed out elevated concentrations of As in one of the two orographic sides. Hair cortisol concentrations were measured using a RIA(Radio Immuno Assay), while minerals were detected using ICP-MS (Inductively Coupled Plasma- Mass Spectrometry). Results showed a negative relationship between cortisol and some mineral concentrations (Li, Co, As, Cd, Cr and Tl) and significant differences in some mineral concentrations between park areas (Al, Co, Cu, Cd and Ni). As, Cr and cortisol differences approached statistical significance. This preliminary study represents a step forward in the study of wildlife allostatic load and a valid method for applications in wildlife management programs, in environmental studies and in public health programs.