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Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract

The importance of whole protein extracts from different types of human teeth in modulating the process of teeth biomineralization is reported. There are two crucial features in protein molecules that result in efficient teeth biomineralization. Firstly, the unique secondary structure characteristics...

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Autores principales: Sharma, Vaibhav, Rani, Komal, Roychoudhury, Ajoy, Chawla, Amita, Nikolajeff, Fredrik, Kumar, Saroj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720696/
https://www.ncbi.nlm.nih.gov/pubmed/31430851
http://dx.doi.org/10.3390/ijms20164035
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author Sharma, Vaibhav
Rani, Komal
Roychoudhury, Ajoy
Chawla, Amita
Nikolajeff, Fredrik
Kumar, Saroj
author_facet Sharma, Vaibhav
Rani, Komal
Roychoudhury, Ajoy
Chawla, Amita
Nikolajeff, Fredrik
Kumar, Saroj
author_sort Sharma, Vaibhav
collection PubMed
description The importance of whole protein extracts from different types of human teeth in modulating the process of teeth biomineralization is reported. There are two crucial features in protein molecules that result in efficient teeth biomineralization. Firstly, the unique secondary structure characteristics within these proteins i.e. the exclusive presence of a large amount of intrinsic disorder and secondly, the presence of post-translational modifications (PTM) like phosphorylation and glycosylation within these protein molecules. The present study accesses the structural implications of PTMs in the tooth proteins through scanning electron microscopy and transmission electron microscopy. The deglycosylated/dephosphorylated protein extracts failed to form higher-order mineralization assemblies. Furthermore, through nanoparticle tracking analysis (NTA) we have shown that dephosphorylation and deglycosylation significantly impact the biomineralization abilities of the protein extract and resulted in smaller sized clusters. Hence, we propose these post-translational modifications are indispensable for the process of teeth biomineralization. In addition to basic science, this study would be worth consideration while designing of biomimetics architecture for an efficient peptide-based teeth remineralization strategy.
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spelling pubmed-67206962019-09-10 Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract Sharma, Vaibhav Rani, Komal Roychoudhury, Ajoy Chawla, Amita Nikolajeff, Fredrik Kumar, Saroj Int J Mol Sci Article The importance of whole protein extracts from different types of human teeth in modulating the process of teeth biomineralization is reported. There are two crucial features in protein molecules that result in efficient teeth biomineralization. Firstly, the unique secondary structure characteristics within these proteins i.e. the exclusive presence of a large amount of intrinsic disorder and secondly, the presence of post-translational modifications (PTM) like phosphorylation and glycosylation within these protein molecules. The present study accesses the structural implications of PTMs in the tooth proteins through scanning electron microscopy and transmission electron microscopy. The deglycosylated/dephosphorylated protein extracts failed to form higher-order mineralization assemblies. Furthermore, through nanoparticle tracking analysis (NTA) we have shown that dephosphorylation and deglycosylation significantly impact the biomineralization abilities of the protein extract and resulted in smaller sized clusters. Hence, we propose these post-translational modifications are indispensable for the process of teeth biomineralization. In addition to basic science, this study would be worth consideration while designing of biomimetics architecture for an efficient peptide-based teeth remineralization strategy. MDPI 2019-08-19 /pmc/articles/PMC6720696/ /pubmed/31430851 http://dx.doi.org/10.3390/ijms20164035 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharma, Vaibhav
Rani, Komal
Roychoudhury, Ajoy
Chawla, Amita
Nikolajeff, Fredrik
Kumar, Saroj
Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title_full Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title_fullStr Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title_full_unstemmed Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title_short Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
title_sort novel insights into regulation of human teeth biomineralization: deciphering the role of post-translational modifications in a tooth protein extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720696/
https://www.ncbi.nlm.nih.gov/pubmed/31430851
http://dx.doi.org/10.3390/ijms20164035
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