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Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues

Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue compositio...

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Autores principales: Sukhikh, Gennady, Chagovets, Vitaliy, Wang, Xinchen, Rodionov, Valeriy, Kometova, Vlada, Tokareva, Alisa, Kononikhin, Alexey, Starodubtseva, Natalia, Chingin, Konstantin, Chen, Huanwen, Frankevich, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720730/
https://www.ncbi.nlm.nih.gov/pubmed/31443190
http://dx.doi.org/10.3390/molecules24162957
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author Sukhikh, Gennady
Chagovets, Vitaliy
Wang, Xinchen
Rodionov, Valeriy
Kometova, Vlada
Tokareva, Alisa
Kononikhin, Alexey
Starodubtseva, Natalia
Chingin, Konstantin
Chen, Huanwen
Frankevich, Vladimir
author_facet Sukhikh, Gennady
Chagovets, Vitaliy
Wang, Xinchen
Rodionov, Valeriy
Kometova, Vlada
Tokareva, Alisa
Kononikhin, Alexey
Starodubtseva, Natalia
Chingin, Konstantin
Chen, Huanwen
Frankevich, Vladimir
author_sort Sukhikh, Gennady
collection PubMed
description Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use.
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spelling pubmed-67207302019-09-10 Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues Sukhikh, Gennady Chagovets, Vitaliy Wang, Xinchen Rodionov, Valeriy Kometova, Vlada Tokareva, Alisa Kononikhin, Alexey Starodubtseva, Natalia Chingin, Konstantin Chen, Huanwen Frankevich, Vladimir Molecules Article Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use. MDPI 2019-08-15 /pmc/articles/PMC6720730/ /pubmed/31443190 http://dx.doi.org/10.3390/molecules24162957 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sukhikh, Gennady
Chagovets, Vitaliy
Wang, Xinchen
Rodionov, Valeriy
Kometova, Vlada
Tokareva, Alisa
Kononikhin, Alexey
Starodubtseva, Natalia
Chingin, Konstantin
Chen, Huanwen
Frankevich, Vladimir
Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title_full Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title_fullStr Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title_full_unstemmed Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title_short Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
title_sort combination of low-temperature electrosurgical unit and extractive electrospray ionization mass spectrometry for molecular profiling and classification of tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720730/
https://www.ncbi.nlm.nih.gov/pubmed/31443190
http://dx.doi.org/10.3390/molecules24162957
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