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Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues
Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue compositio...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720730/ https://www.ncbi.nlm.nih.gov/pubmed/31443190 http://dx.doi.org/10.3390/molecules24162957 |
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author | Sukhikh, Gennady Chagovets, Vitaliy Wang, Xinchen Rodionov, Valeriy Kometova, Vlada Tokareva, Alisa Kononikhin, Alexey Starodubtseva, Natalia Chingin, Konstantin Chen, Huanwen Frankevich, Vladimir |
author_facet | Sukhikh, Gennady Chagovets, Vitaliy Wang, Xinchen Rodionov, Valeriy Kometova, Vlada Tokareva, Alisa Kononikhin, Alexey Starodubtseva, Natalia Chingin, Konstantin Chen, Huanwen Frankevich, Vladimir |
author_sort | Sukhikh, Gennady |
collection | PubMed |
description | Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use. |
format | Online Article Text |
id | pubmed-6720730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67207302019-09-10 Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues Sukhikh, Gennady Chagovets, Vitaliy Wang, Xinchen Rodionov, Valeriy Kometova, Vlada Tokareva, Alisa Kononikhin, Alexey Starodubtseva, Natalia Chingin, Konstantin Chen, Huanwen Frankevich, Vladimir Molecules Article Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use. MDPI 2019-08-15 /pmc/articles/PMC6720730/ /pubmed/31443190 http://dx.doi.org/10.3390/molecules24162957 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sukhikh, Gennady Chagovets, Vitaliy Wang, Xinchen Rodionov, Valeriy Kometova, Vlada Tokareva, Alisa Kononikhin, Alexey Starodubtseva, Natalia Chingin, Konstantin Chen, Huanwen Frankevich, Vladimir Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title | Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title_full | Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title_fullStr | Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title_full_unstemmed | Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title_short | Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues |
title_sort | combination of low-temperature electrosurgical unit and extractive electrospray ionization mass spectrometry for molecular profiling and classification of tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720730/ https://www.ncbi.nlm.nih.gov/pubmed/31443190 http://dx.doi.org/10.3390/molecules24162957 |
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