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Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria
The principle of animal wellbeing, which states that animals should be free from pain, injury, and disease, is difficult to maintain, because microorganisms are most frequently found to be resistant or multi-resistant to drugs. The secondary metabolites of plants are an alternative for the treatment...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721091/ https://www.ncbi.nlm.nih.gov/pubmed/31426583 http://dx.doi.org/10.3390/molecules24162992 |
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author | González-Alamilla, Eddy Nathalye Gonzalez-Cortazar, Manases Valladares-Carranza, Benjamín Rivas-Jacobo, Marco Antonio Herrera-Corredor, Camelia Alejandra Ojeda-Ramírez, Deyanira Zaragoza-Bastida, Adrian Rivero-Perez, Nallely |
author_facet | González-Alamilla, Eddy Nathalye Gonzalez-Cortazar, Manases Valladares-Carranza, Benjamín Rivas-Jacobo, Marco Antonio Herrera-Corredor, Camelia Alejandra Ojeda-Ramírez, Deyanira Zaragoza-Bastida, Adrian Rivero-Perez, Nallely |
author_sort | González-Alamilla, Eddy Nathalye |
collection | PubMed |
description | The principle of animal wellbeing, which states that animals should be free from pain, injury, and disease, is difficult to maintain, because microorganisms are most frequently found to be resistant or multi-resistant to drugs. The secondary metabolites of plants are an alternative for the treatment of these microorganisms. The aim of this work was to determine the antibacterial effect of Salix babylonica L. hydroalcoholic extract (SBHE) against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes, and identify the compounds associated with the activity. The SBHE showed activity against the three strains, and was subjected to a bipartition, obtaining aqueous fraction (ASB) with moderate activity and organic fraction (ACSB) with good activity against the three strains. The chromatographic separation of ACSB, allowed us to obtain ten fractions (F1AC to F10AC), and only three showed activity (F7AC, F8AC and F10AC). In F7AC, five compounds were identified preliminary by GC-MS, in F8AC and F10AC were identified luteolin (1) and luteolin 7-O-glucoside (2) by HPLC, respectively. The best antibacterial activity was obtained with F7AC (Listeria monocytogenes; MIC: 0.78 mg/mL, MBC: 0.78 mg/mL) and F8AC (Staphylococcus aureus; MIC: 0.39 mg/mL; MBC: 0.78 mg/mL). The results indicated that the compounds obtained from SBHE can be used as an alternative treatment against these microorganisms and, by this mechanism, contribute to animal and human health. |
format | Online Article Text |
id | pubmed-6721091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67210912019-09-10 Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria González-Alamilla, Eddy Nathalye Gonzalez-Cortazar, Manases Valladares-Carranza, Benjamín Rivas-Jacobo, Marco Antonio Herrera-Corredor, Camelia Alejandra Ojeda-Ramírez, Deyanira Zaragoza-Bastida, Adrian Rivero-Perez, Nallely Molecules Article The principle of animal wellbeing, which states that animals should be free from pain, injury, and disease, is difficult to maintain, because microorganisms are most frequently found to be resistant or multi-resistant to drugs. The secondary metabolites of plants are an alternative for the treatment of these microorganisms. The aim of this work was to determine the antibacterial effect of Salix babylonica L. hydroalcoholic extract (SBHE) against Escherichia coli, Staphylococcus aureus and Listeria monocytogenes, and identify the compounds associated with the activity. The SBHE showed activity against the three strains, and was subjected to a bipartition, obtaining aqueous fraction (ASB) with moderate activity and organic fraction (ACSB) with good activity against the three strains. The chromatographic separation of ACSB, allowed us to obtain ten fractions (F1AC to F10AC), and only three showed activity (F7AC, F8AC and F10AC). In F7AC, five compounds were identified preliminary by GC-MS, in F8AC and F10AC were identified luteolin (1) and luteolin 7-O-glucoside (2) by HPLC, respectively. The best antibacterial activity was obtained with F7AC (Listeria monocytogenes; MIC: 0.78 mg/mL, MBC: 0.78 mg/mL) and F8AC (Staphylococcus aureus; MIC: 0.39 mg/mL; MBC: 0.78 mg/mL). The results indicated that the compounds obtained from SBHE can be used as an alternative treatment against these microorganisms and, by this mechanism, contribute to animal and human health. MDPI 2019-08-18 /pmc/articles/PMC6721091/ /pubmed/31426583 http://dx.doi.org/10.3390/molecules24162992 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article González-Alamilla, Eddy Nathalye Gonzalez-Cortazar, Manases Valladares-Carranza, Benjamín Rivas-Jacobo, Marco Antonio Herrera-Corredor, Camelia Alejandra Ojeda-Ramírez, Deyanira Zaragoza-Bastida, Adrian Rivero-Perez, Nallely Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title | Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title_full | Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title_fullStr | Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title_full_unstemmed | Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title_short | Chemical Constituents of Salix babylonica L. and Their Antibacterial Activity Against Gram-Positive and Gram-Negative Animal Bacteria |
title_sort | chemical constituents of salix babylonica l. and their antibacterial activity against gram-positive and gram-negative animal bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721091/ https://www.ncbi.nlm.nih.gov/pubmed/31426583 http://dx.doi.org/10.3390/molecules24162992 |
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