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Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains
BACKGROUND: Salmonella is generally considered as a human pathogen causing typhoid fever and gastrointestinal infections called salmonellosis, with S. Enteritidis and S. Typhimurium strains as the main causative agents. Salmonella enterica strains have a wide host array including humans, birds, pigs...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721270/ https://www.ncbi.nlm.nih.gov/pubmed/31477105 http://dx.doi.org/10.1186/s12917-019-2019-1 |
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author | Dudek, Bartłomiej Książczyk, Marta Krzyżewska, Eva Rogala, Klaudia Kuczkowski, Maciej Woźniak-Biel, Anna Korzekwa, Kamila Korzeniowska-Kowal, Agnieszka Ratajszczak, Radosław Wieliczko, Alina Rybka, Jacek Bugla-Płoskońska, Gabriela |
author_facet | Dudek, Bartłomiej Książczyk, Marta Krzyżewska, Eva Rogala, Klaudia Kuczkowski, Maciej Woźniak-Biel, Anna Korzekwa, Kamila Korzeniowska-Kowal, Agnieszka Ratajszczak, Radosław Wieliczko, Alina Rybka, Jacek Bugla-Płoskońska, Gabriela |
author_sort | Dudek, Bartłomiej |
collection | PubMed |
description | BACKGROUND: Salmonella is generally considered as a human pathogen causing typhoid fever and gastrointestinal infections called salmonellosis, with S. Enteritidis and S. Typhimurium strains as the main causative agents. Salmonella enterica strains have a wide host array including humans, birds, pigs, horses, dogs, cats, reptiles, amphibians and insects. Up to 90% of reptiles are the carriers of one or more serovars of Salmonella. Extraintestinal bacterial infections associated with reptiles pose serious health threat to humans. The import of exotic species of reptiles as pet animals to Europe correlates with the emergence of Salmonella serotypes, which not found previously in European countries. The presented study is a new report about Salmonella serotypes associated with exotic reptiles in Poland. The goal of this research was to examine the zoonotic potential of Salmonella strains isolated from reptiles by comparative analysis with S. Enteritidis strains occurring in human population and causing salmonellosis. RESULTS: The main findings of our work show that exotic reptiles are asymptomatic carriers of Salmonella serovars other than correlated with salmonellosis in humans (S. Enteritidis, S. Typhimurium). Among the isolated Salmonella strains we identified serovars that have not been reported earlier in Poland, for example belonging to subspecies diarizonae and salamae. Restriction analysis with Pulsed-field Gel Electrophoresis (PFGE), showed a great diversity among Salmonella strains isolated from reptiles. Almost all tested strains had distinct restriction patterns. While S. Enteritidis strains were quite homogeneous in term of phylogenetic relations. Most of the tested VGs were common for the two tested groups of Salmonella strains. CONCLUSIONS: The obtained results show that Salmonella strains isolated from reptiles share most of virulence genes with the S. Enteritidis strains and exhibit a greater phylogenetic diversity than the tested S. Enteritidis population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-019-2019-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6721270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67212702019-09-10 Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains Dudek, Bartłomiej Książczyk, Marta Krzyżewska, Eva Rogala, Klaudia Kuczkowski, Maciej Woźniak-Biel, Anna Korzekwa, Kamila Korzeniowska-Kowal, Agnieszka Ratajszczak, Radosław Wieliczko, Alina Rybka, Jacek Bugla-Płoskońska, Gabriela BMC Vet Res Research Article BACKGROUND: Salmonella is generally considered as a human pathogen causing typhoid fever and gastrointestinal infections called salmonellosis, with S. Enteritidis and S. Typhimurium strains as the main causative agents. Salmonella enterica strains have a wide host array including humans, birds, pigs, horses, dogs, cats, reptiles, amphibians and insects. Up to 90% of reptiles are the carriers of one or more serovars of Salmonella. Extraintestinal bacterial infections associated with reptiles pose serious health threat to humans. The import of exotic species of reptiles as pet animals to Europe correlates with the emergence of Salmonella serotypes, which not found previously in European countries. The presented study is a new report about Salmonella serotypes associated with exotic reptiles in Poland. The goal of this research was to examine the zoonotic potential of Salmonella strains isolated from reptiles by comparative analysis with S. Enteritidis strains occurring in human population and causing salmonellosis. RESULTS: The main findings of our work show that exotic reptiles are asymptomatic carriers of Salmonella serovars other than correlated with salmonellosis in humans (S. Enteritidis, S. Typhimurium). Among the isolated Salmonella strains we identified serovars that have not been reported earlier in Poland, for example belonging to subspecies diarizonae and salamae. Restriction analysis with Pulsed-field Gel Electrophoresis (PFGE), showed a great diversity among Salmonella strains isolated from reptiles. Almost all tested strains had distinct restriction patterns. While S. Enteritidis strains were quite homogeneous in term of phylogenetic relations. Most of the tested VGs were common for the two tested groups of Salmonella strains. CONCLUSIONS: The obtained results show that Salmonella strains isolated from reptiles share most of virulence genes with the S. Enteritidis strains and exhibit a greater phylogenetic diversity than the tested S. Enteritidis population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12917-019-2019-1) contains supplementary material, which is available to authorized users. BioMed Central 2019-09-02 /pmc/articles/PMC6721270/ /pubmed/31477105 http://dx.doi.org/10.1186/s12917-019-2019-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Dudek, Bartłomiej Książczyk, Marta Krzyżewska, Eva Rogala, Klaudia Kuczkowski, Maciej Woźniak-Biel, Anna Korzekwa, Kamila Korzeniowska-Kowal, Agnieszka Ratajszczak, Radosław Wieliczko, Alina Rybka, Jacek Bugla-Płoskońska, Gabriela Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title | Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title_full | Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title_fullStr | Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title_full_unstemmed | Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title_short | Comparison of the phylogenetic analysis of PFGE profiles and the characteristic of virulence genes in clinical and reptile associated Salmonella strains |
title_sort | comparison of the phylogenetic analysis of pfge profiles and the characteristic of virulence genes in clinical and reptile associated salmonella strains |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721270/ https://www.ncbi.nlm.nih.gov/pubmed/31477105 http://dx.doi.org/10.1186/s12917-019-2019-1 |
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