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Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I

Complex regional pain syndrome type I (CRPS-I) is a chronic painful condition. We investigated whether manual therapy (MT), in a chronic post-ischemia pain (CPIP) model, is capable of reducing pain behavior and oxidative stress. Male Swiss mice were subjected to ischemia-reperfusion (IR) to mimic CR...

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Autores principales: Salgado, Afonso S. I., Stramosk, Juliana, Ludtke, Daniela D., Kuci, Ana C. C., Salm, Daiana C., Ceci, Lisandro A., Petronilho, Fabricia, Florentino, Drielly, Danielski, Lucineia G., Gassenferth, Aline, Souza, Luana R., Rezin, Gislaine T., Santos, Adair R. S., Mazzardo-Martins, Leidiane, Reed, William R., Martins, Daniel F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721404/
https://www.ncbi.nlm.nih.gov/pubmed/31405150
http://dx.doi.org/10.3390/brainsci9080197
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author Salgado, Afonso S. I.
Stramosk, Juliana
Ludtke, Daniela D.
Kuci, Ana C. C.
Salm, Daiana C.
Ceci, Lisandro A.
Petronilho, Fabricia
Florentino, Drielly
Danielski, Lucineia G.
Gassenferth, Aline
Souza, Luana R.
Rezin, Gislaine T.
Santos, Adair R. S.
Mazzardo-Martins, Leidiane
Reed, William R.
Martins, Daniel F.
author_facet Salgado, Afonso S. I.
Stramosk, Juliana
Ludtke, Daniela D.
Kuci, Ana C. C.
Salm, Daiana C.
Ceci, Lisandro A.
Petronilho, Fabricia
Florentino, Drielly
Danielski, Lucineia G.
Gassenferth, Aline
Souza, Luana R.
Rezin, Gislaine T.
Santos, Adair R. S.
Mazzardo-Martins, Leidiane
Reed, William R.
Martins, Daniel F.
author_sort Salgado, Afonso S. I.
collection PubMed
description Complex regional pain syndrome type I (CRPS-I) is a chronic painful condition. We investigated whether manual therapy (MT), in a chronic post-ischemia pain (CPIP) model, is capable of reducing pain behavior and oxidative stress. Male Swiss mice were subjected to ischemia-reperfusion (IR) to mimic CRPS-I. Animals received ankle joint mobilization 48h after the IR procedure, and response to mechanical stimuli was evaluated. For biochemical analyses, mitochondrial function as well as oxidative stress thiobarbituric acid reactive substances (TBARS), protein carbonyls, antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) levels were determined. IR induced mechanical hyperalgesia which was subsequently reduced by acute MT treatment. The concentrations of oxidative stress parameters were increased following IR with MT treatment preventing these increases in malondialdehyde (MDA) and carbonyls protein. IR diminished the levels of SOD and CAT activity and MT treatment prevented this decrease in CAT but not in SOD activity. IR also diminished mitochondrial complex activity, and MT treatment was ineffective in preventing this decrease. In conclusion, repeated sessions of MT resulted in antihyperalgesic effects mediated, at least partially, through the prevention of an increase of MDA and protein carbonyls levels and an improvement in the antioxidant defense system.
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spelling pubmed-67214042019-09-10 Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I Salgado, Afonso S. I. Stramosk, Juliana Ludtke, Daniela D. Kuci, Ana C. C. Salm, Daiana C. Ceci, Lisandro A. Petronilho, Fabricia Florentino, Drielly Danielski, Lucineia G. Gassenferth, Aline Souza, Luana R. Rezin, Gislaine T. Santos, Adair R. S. Mazzardo-Martins, Leidiane Reed, William R. Martins, Daniel F. Brain Sci Article Complex regional pain syndrome type I (CRPS-I) is a chronic painful condition. We investigated whether manual therapy (MT), in a chronic post-ischemia pain (CPIP) model, is capable of reducing pain behavior and oxidative stress. Male Swiss mice were subjected to ischemia-reperfusion (IR) to mimic CRPS-I. Animals received ankle joint mobilization 48h after the IR procedure, and response to mechanical stimuli was evaluated. For biochemical analyses, mitochondrial function as well as oxidative stress thiobarbituric acid reactive substances (TBARS), protein carbonyls, antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) levels were determined. IR induced mechanical hyperalgesia which was subsequently reduced by acute MT treatment. The concentrations of oxidative stress parameters were increased following IR with MT treatment preventing these increases in malondialdehyde (MDA) and carbonyls protein. IR diminished the levels of SOD and CAT activity and MT treatment prevented this decrease in CAT but not in SOD activity. IR also diminished mitochondrial complex activity, and MT treatment was ineffective in preventing this decrease. In conclusion, repeated sessions of MT resulted in antihyperalgesic effects mediated, at least partially, through the prevention of an increase of MDA and protein carbonyls levels and an improvement in the antioxidant defense system. MDPI 2019-08-10 /pmc/articles/PMC6721404/ /pubmed/31405150 http://dx.doi.org/10.3390/brainsci9080197 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salgado, Afonso S. I.
Stramosk, Juliana
Ludtke, Daniela D.
Kuci, Ana C. C.
Salm, Daiana C.
Ceci, Lisandro A.
Petronilho, Fabricia
Florentino, Drielly
Danielski, Lucineia G.
Gassenferth, Aline
Souza, Luana R.
Rezin, Gislaine T.
Santos, Adair R. S.
Mazzardo-Martins, Leidiane
Reed, William R.
Martins, Daniel F.
Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title_full Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title_fullStr Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title_full_unstemmed Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title_short Manual Therapy Reduces Pain Behavior and Oxidative Stress in a Murine Model of Complex Regional Pain Syndrome Type I
title_sort manual therapy reduces pain behavior and oxidative stress in a murine model of complex regional pain syndrome type i
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721404/
https://www.ncbi.nlm.nih.gov/pubmed/31405150
http://dx.doi.org/10.3390/brainsci9080197
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