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Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model

The aim of the study is to explore the protective effect of new gelatin (NG, Xin'ejiao in China) on hematopoietic injury caused by chemotherapy. Zebrafish, at 48 hours post fertilization (hpf), was treated with different chemotherapeutic drugs to establish the zebrafish hematopoietic damage mod...

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Autores principales: Han, Liwen, Kong, Haotian, Liu, Fasheng, Li, Xiaobin, Zhang, Shanshan, Zhang, Xuanming, Wu, Yong, Yang, Hua, Zhang, Aiping, Liu, Kechun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721477/
https://www.ncbi.nlm.nih.gov/pubmed/31531120
http://dx.doi.org/10.1155/2019/8918943
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author Han, Liwen
Kong, Haotian
Liu, Fasheng
Li, Xiaobin
Zhang, Shanshan
Zhang, Xuanming
Wu, Yong
Yang, Hua
Zhang, Aiping
Liu, Kechun
author_facet Han, Liwen
Kong, Haotian
Liu, Fasheng
Li, Xiaobin
Zhang, Shanshan
Zhang, Xuanming
Wu, Yong
Yang, Hua
Zhang, Aiping
Liu, Kechun
author_sort Han, Liwen
collection PubMed
description The aim of the study is to explore the protective effect of new gelatin (NG, Xin'ejiao in China) on hematopoietic injury caused by chemotherapy. Zebrafish, at 48 hours post fertilization (hpf), was treated with different chemotherapeutic drugs to establish the zebrafish hematopoietic damage model with reduced thrombocytes and erythrocytes. The protecting effects of NG on the thrombocytes and erythrocytes were observed, respectively, on zebrafish models. Then, the RT-PCR method was used to detect the change of mRNA level of the hematopoiesis-related cytokines scl1, c-myb, pu.1, GATA1, and runx1 genes. The results showed that 50 μg·mL(−1) and 100 μg·mL(−1) NG rescued and increased the thrombocytes numbers induced by vinorelbine (NVB) and chloramphenicol (CHL) and the erythrocytes numbers induced by methotrexate (MTX), doxorubicin (ADM), and mechlorethamine hydrochloride (MH) in zebrafish models. Meanwhile, the mRNA expression of scl1, c-myb, and GATA1 genes in the NG treatment group was raised compared with the MTX treatment group. Also, the mRNA expression of pu.1 and Runx1 in the NG treatment group was reduced compared with the MTX treatment group. In consequence, traditional Chinese medicine NG showed a certain degree protective effect on hematopoiesis injury induced by chemotherapy in this study, which may depend on the promotion of erythrocytes proliferation and the regulation of the hematopoietic genes level.
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spelling pubmed-67214772019-09-17 Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model Han, Liwen Kong, Haotian Liu, Fasheng Li, Xiaobin Zhang, Shanshan Zhang, Xuanming Wu, Yong Yang, Hua Zhang, Aiping Liu, Kechun Evid Based Complement Alternat Med Research Article The aim of the study is to explore the protective effect of new gelatin (NG, Xin'ejiao in China) on hematopoietic injury caused by chemotherapy. Zebrafish, at 48 hours post fertilization (hpf), was treated with different chemotherapeutic drugs to establish the zebrafish hematopoietic damage model with reduced thrombocytes and erythrocytes. The protecting effects of NG on the thrombocytes and erythrocytes were observed, respectively, on zebrafish models. Then, the RT-PCR method was used to detect the change of mRNA level of the hematopoiesis-related cytokines scl1, c-myb, pu.1, GATA1, and runx1 genes. The results showed that 50 μg·mL(−1) and 100 μg·mL(−1) NG rescued and increased the thrombocytes numbers induced by vinorelbine (NVB) and chloramphenicol (CHL) and the erythrocytes numbers induced by methotrexate (MTX), doxorubicin (ADM), and mechlorethamine hydrochloride (MH) in zebrafish models. Meanwhile, the mRNA expression of scl1, c-myb, and GATA1 genes in the NG treatment group was raised compared with the MTX treatment group. Also, the mRNA expression of pu.1 and Runx1 in the NG treatment group was reduced compared with the MTX treatment group. In consequence, traditional Chinese medicine NG showed a certain degree protective effect on hematopoiesis injury induced by chemotherapy in this study, which may depend on the promotion of erythrocytes proliferation and the regulation of the hematopoietic genes level. Hindawi 2019-08-21 /pmc/articles/PMC6721477/ /pubmed/31531120 http://dx.doi.org/10.1155/2019/8918943 Text en Copyright © 2019 Liwen Han et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Han, Liwen
Kong, Haotian
Liu, Fasheng
Li, Xiaobin
Zhang, Shanshan
Zhang, Xuanming
Wu, Yong
Yang, Hua
Zhang, Aiping
Liu, Kechun
Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title_full Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title_fullStr Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title_full_unstemmed Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title_short Protective Effect and Mechanisms of New Gelatin on Chemotherapy-Induced Hematopoietic Injury Zebrafish Model
title_sort protective effect and mechanisms of new gelatin on chemotherapy-induced hematopoietic injury zebrafish model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721477/
https://www.ncbi.nlm.nih.gov/pubmed/31531120
http://dx.doi.org/10.1155/2019/8918943
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