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Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight

Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic pla...

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Autores principales: Hong, Yu-Hui, Meng, Jun, He, Xiao-Li, Zhang, Yuan-Yuan, Luan, Yu-Shi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721620/
https://www.ncbi.nlm.nih.gov/pubmed/31382588
http://dx.doi.org/10.3390/cells8080822
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author Hong, Yu-Hui
Meng, Jun
He, Xiao-Li
Zhang, Yuan-Yuan
Luan, Yu-Shi
author_facet Hong, Yu-Hui
Meng, Jun
He, Xiao-Li
Zhang, Yuan-Yuan
Luan, Yu-Shi
author_sort Hong, Yu-Hui
collection PubMed
description Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels.
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spelling pubmed-67216202019-09-10 Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight Hong, Yu-Hui Meng, Jun He, Xiao-Li Zhang, Yuan-Yuan Luan, Yu-Shi Cells Article Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels. MDPI 2019-08-03 /pmc/articles/PMC6721620/ /pubmed/31382588 http://dx.doi.org/10.3390/cells8080822 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hong, Yu-Hui
Meng, Jun
He, Xiao-Li
Zhang, Yuan-Yuan
Luan, Yu-Shi
Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title_full Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title_fullStr Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title_full_unstemmed Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title_short Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
title_sort overexpression of mir482c in tomato induces enhanced susceptibility to late blight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721620/
https://www.ncbi.nlm.nih.gov/pubmed/31382588
http://dx.doi.org/10.3390/cells8080822
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