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Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight
Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic pla...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721620/ https://www.ncbi.nlm.nih.gov/pubmed/31382588 http://dx.doi.org/10.3390/cells8080822 |
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author | Hong, Yu-Hui Meng, Jun He, Xiao-Li Zhang, Yuan-Yuan Luan, Yu-Shi |
author_facet | Hong, Yu-Hui Meng, Jun He, Xiao-Li Zhang, Yuan-Yuan Luan, Yu-Shi |
author_sort | Hong, Yu-Hui |
collection | PubMed |
description | Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels. |
format | Online Article Text |
id | pubmed-6721620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67216202019-09-10 Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight Hong, Yu-Hui Meng, Jun He, Xiao-Li Zhang, Yuan-Yuan Luan, Yu-Shi Cells Article Tomato is the highest-value fruit/vegetable crop worldwide. However, the quality and yield of tomatoes are severely affected by late blight. MicroRNA482s (miR482s) are involved in the plant’s immune system. In this study, miR482c was transiently and stably overexpressed in tomatoes in transgenic plants to explore its mechanism in tomato resistance against late blight. Transgenic tomato plants with transiently overexpressed miR482c displayed a larger lesion area than the control plants upon infection. Furthermore, compared with wild-type (WT) tomato plants, the transgenic tomato plants stably overexpressing miR482c displayed a decreased expression of target genes accompanied by lower peroxidase (POD), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) activity activities and higher malondialdehyde (MDA) content, thereby leading to a decline in reactive oxygen species (ROS) scavenging ability and aggravating the damage of lipid peroxidation product accumulation on the cell membrane, eventually enhancing plant susceptibility. This finding indicates that miR482c may act as a negative regulator in tomato resistance by regulating nucleotide binding sites and leucine-rich repeat (NBS-LRR) expression levels and ROS levels. MDPI 2019-08-03 /pmc/articles/PMC6721620/ /pubmed/31382588 http://dx.doi.org/10.3390/cells8080822 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hong, Yu-Hui Meng, Jun He, Xiao-Li Zhang, Yuan-Yuan Luan, Yu-Shi Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title | Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_full | Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_fullStr | Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_full_unstemmed | Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_short | Overexpression of MiR482c in Tomato Induces Enhanced Susceptibility to Late Blight |
title_sort | overexpression of mir482c in tomato induces enhanced susceptibility to late blight |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721620/ https://www.ncbi.nlm.nih.gov/pubmed/31382588 http://dx.doi.org/10.3390/cells8080822 |
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