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Characterization of Cell Glycocalyx with Mass Spectrometry Methods

The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function. Crucial intercellular processes, such as signal transduction and immune protection, are mediated by cell surface glyco...

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Detalles Bibliográficos
Autores principales: Li, Qiongyu, Xie, Yixuan, Wong, Maurice, Lebrilla, Carlito B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721671/
https://www.ncbi.nlm.nih.gov/pubmed/31412618
http://dx.doi.org/10.3390/cells8080882
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author Li, Qiongyu
Xie, Yixuan
Wong, Maurice
Lebrilla, Carlito B.
author_facet Li, Qiongyu
Xie, Yixuan
Wong, Maurice
Lebrilla, Carlito B.
author_sort Li, Qiongyu
collection PubMed
description The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function. Crucial intercellular processes, such as signal transduction and immune protection, are mediated by cell surface glycosylation, which is comprised of large biomolecules, including glycoproteins and glycosphingolipids. Because perturbations in glycosylation could result in dysfunction of cells and are related to diseases, the analysis of surface glycosylation is critical for understanding pathogenic mechanisms and can further lead to biomarker discovery. Different mass spectrometry-based techniques have been developed for glycan analysis, ranging from highly specific, targeted approaches to more comprehensive profiling studies. In this review, we summarized the work conducted for extensive analysis of cell membrane glycosylation, particularly those employing liquid chromatography with mass spectrometry (LC-MS) in combination with various sample preparation techniques.
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spelling pubmed-67216712019-09-10 Characterization of Cell Glycocalyx with Mass Spectrometry Methods Li, Qiongyu Xie, Yixuan Wong, Maurice Lebrilla, Carlito B. Cells Review The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function. Crucial intercellular processes, such as signal transduction and immune protection, are mediated by cell surface glycosylation, which is comprised of large biomolecules, including glycoproteins and glycosphingolipids. Because perturbations in glycosylation could result in dysfunction of cells and are related to diseases, the analysis of surface glycosylation is critical for understanding pathogenic mechanisms and can further lead to biomarker discovery. Different mass spectrometry-based techniques have been developed for glycan analysis, ranging from highly specific, targeted approaches to more comprehensive profiling studies. In this review, we summarized the work conducted for extensive analysis of cell membrane glycosylation, particularly those employing liquid chromatography with mass spectrometry (LC-MS) in combination with various sample preparation techniques. MDPI 2019-08-13 /pmc/articles/PMC6721671/ /pubmed/31412618 http://dx.doi.org/10.3390/cells8080882 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Li, Qiongyu
Xie, Yixuan
Wong, Maurice
Lebrilla, Carlito B.
Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title_full Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title_fullStr Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title_full_unstemmed Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title_short Characterization of Cell Glycocalyx with Mass Spectrometry Methods
title_sort characterization of cell glycocalyx with mass spectrometry methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721671/
https://www.ncbi.nlm.nih.gov/pubmed/31412618
http://dx.doi.org/10.3390/cells8080882
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