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Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice

The role of uncoupling protein-3 (UCP3) in energy and lipid metabolism was investigated. Male wild-type (WT) and UCP3-null (KO) mice that were housed at thermoneutrality (30 °C) were used as the animal model. In KO mice, the ability of skeletal muscle mitochondria to oxidize fatty acids (but not pyr...

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Autores principales: Lombardi, Assunta, Busiello, Rosa Anna, De Matteis, Rita, Lionetti, Lillà, Savarese, Sabrina, Moreno, Maria, Gentile, Alessandra, Silvestri, Elena, Senese, Rosalba, de Lange, Pieter, Cioffi, Federica, Lanni, Antonia, Goglia, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721699/
https://www.ncbi.nlm.nih.gov/pubmed/31426456
http://dx.doi.org/10.3390/cells8080916
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author Lombardi, Assunta
Busiello, Rosa Anna
De Matteis, Rita
Lionetti, Lillà
Savarese, Sabrina
Moreno, Maria
Gentile, Alessandra
Silvestri, Elena
Senese, Rosalba
de Lange, Pieter
Cioffi, Federica
Lanni, Antonia
Goglia, Fernando
author_facet Lombardi, Assunta
Busiello, Rosa Anna
De Matteis, Rita
Lionetti, Lillà
Savarese, Sabrina
Moreno, Maria
Gentile, Alessandra
Silvestri, Elena
Senese, Rosalba
de Lange, Pieter
Cioffi, Federica
Lanni, Antonia
Goglia, Fernando
author_sort Lombardi, Assunta
collection PubMed
description The role of uncoupling protein-3 (UCP3) in energy and lipid metabolism was investigated. Male wild-type (WT) and UCP3-null (KO) mice that were housed at thermoneutrality (30 °C) were used as the animal model. In KO mice, the ability of skeletal muscle mitochondria to oxidize fatty acids (but not pyruvate or succinate) was reduced. At whole animal level, adult KO mice presented blunted resting metabolic rates, energy expenditure, food intake, and the use of lipids as metabolic substrates. When WT and KO mice were fed with a standard/low-fat diet for 80 days, since weaning, they showed similar weight gain and body composition. Interestingly, KO mice showed lower fat accumulation in visceral adipose tissue and higher ectopic fat accumulation in liver and skeletal muscle. When fed with a high-fat diet for 80 days, since weaning, KO mice showed enhanced energy efficiency and an increased lipid gain (thus leading to a change in body composition between the two genotypes). We conclude that UCP3 plays a role in energy and lipid homeostasis and in preserving lean tissues by lipotoxicity, in mice that were housed at thermoneutrality.
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spelling pubmed-67216992019-09-10 Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice Lombardi, Assunta Busiello, Rosa Anna De Matteis, Rita Lionetti, Lillà Savarese, Sabrina Moreno, Maria Gentile, Alessandra Silvestri, Elena Senese, Rosalba de Lange, Pieter Cioffi, Federica Lanni, Antonia Goglia, Fernando Cells Article The role of uncoupling protein-3 (UCP3) in energy and lipid metabolism was investigated. Male wild-type (WT) and UCP3-null (KO) mice that were housed at thermoneutrality (30 °C) were used as the animal model. In KO mice, the ability of skeletal muscle mitochondria to oxidize fatty acids (but not pyruvate or succinate) was reduced. At whole animal level, adult KO mice presented blunted resting metabolic rates, energy expenditure, food intake, and the use of lipids as metabolic substrates. When WT and KO mice were fed with a standard/low-fat diet for 80 days, since weaning, they showed similar weight gain and body composition. Interestingly, KO mice showed lower fat accumulation in visceral adipose tissue and higher ectopic fat accumulation in liver and skeletal muscle. When fed with a high-fat diet for 80 days, since weaning, KO mice showed enhanced energy efficiency and an increased lipid gain (thus leading to a change in body composition between the two genotypes). We conclude that UCP3 plays a role in energy and lipid homeostasis and in preserving lean tissues by lipotoxicity, in mice that were housed at thermoneutrality. MDPI 2019-08-17 /pmc/articles/PMC6721699/ /pubmed/31426456 http://dx.doi.org/10.3390/cells8080916 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lombardi, Assunta
Busiello, Rosa Anna
De Matteis, Rita
Lionetti, Lillà
Savarese, Sabrina
Moreno, Maria
Gentile, Alessandra
Silvestri, Elena
Senese, Rosalba
de Lange, Pieter
Cioffi, Federica
Lanni, Antonia
Goglia, Fernando
Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title_full Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title_fullStr Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title_full_unstemmed Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title_short Absence of Uncoupling Protein-3 at Thermoneutrality Impacts Lipid Handling and Energy Homeostasis in Mice
title_sort absence of uncoupling protein-3 at thermoneutrality impacts lipid handling and energy homeostasis in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721699/
https://www.ncbi.nlm.nih.gov/pubmed/31426456
http://dx.doi.org/10.3390/cells8080916
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