Cargando…
MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus
The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally induced aestivation in marine invertebrates. We hypothesized that mechanisms that arrest energy-expensive cell cycle activity would contribute significantly to establishing the hypometabolic state...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721757/ https://www.ncbi.nlm.nih.gov/pubmed/31390757 http://dx.doi.org/10.3390/cells8080843 |
_version_ | 1783448414595317760 |
---|---|
author | Wang, Shanshan Chen, Muyan Yin, Yingchao Storey, Kenneth B. |
author_facet | Wang, Shanshan Chen, Muyan Yin, Yingchao Storey, Kenneth B. |
author_sort | Wang, Shanshan |
collection | PubMed |
description | The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally induced aestivation in marine invertebrates. We hypothesized that mechanisms that arrest energy-expensive cell cycle activity would contribute significantly to establishing the hypometabolic state during aestivation. Cyclin A is a core and particularly interesting cell cycle regulator that functions in both the S phase and in mitosis. In the present study, negative relationships between miR-200-3p and AjCA expressions were detected at both the transcriptional and the translational levels during aestivation in A. japonicus. Dual-luciferase reporter assays confirmed the targeted location of the miR-200-3p binding site within the AjCA gene transcript. Furthermore, gain- and loss-of-function experiments were conducted in vivo with sea cucumbers to verify the interaction between miR-200-3p and AjCA in intestine tissue by qRT-PCR and Western blotting. The results show that the overexpression of miR-200-3p mimics suppressed AjCA transcript levels and translated protein production, whereas transfection with a miR-200-3p inhibitor enhanced both AjCA mRNA and AjCA protein in A. japonicus intestine. Our findings suggested a potential mechanism that reversibly arrests cell cycle progression during aestivation, which may center on miR-200-3p inhibitory control over the translation of cyclin A mRNA transcripts. |
format | Online Article Text |
id | pubmed-6721757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67217572019-09-10 MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus Wang, Shanshan Chen, Muyan Yin, Yingchao Storey, Kenneth B. Cells Article The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally induced aestivation in marine invertebrates. We hypothesized that mechanisms that arrest energy-expensive cell cycle activity would contribute significantly to establishing the hypometabolic state during aestivation. Cyclin A is a core and particularly interesting cell cycle regulator that functions in both the S phase and in mitosis. In the present study, negative relationships between miR-200-3p and AjCA expressions were detected at both the transcriptional and the translational levels during aestivation in A. japonicus. Dual-luciferase reporter assays confirmed the targeted location of the miR-200-3p binding site within the AjCA gene transcript. Furthermore, gain- and loss-of-function experiments were conducted in vivo with sea cucumbers to verify the interaction between miR-200-3p and AjCA in intestine tissue by qRT-PCR and Western blotting. The results show that the overexpression of miR-200-3p mimics suppressed AjCA transcript levels and translated protein production, whereas transfection with a miR-200-3p inhibitor enhanced both AjCA mRNA and AjCA protein in A. japonicus intestine. Our findings suggested a potential mechanism that reversibly arrests cell cycle progression during aestivation, which may center on miR-200-3p inhibitory control over the translation of cyclin A mRNA transcripts. MDPI 2019-08-06 /pmc/articles/PMC6721757/ /pubmed/31390757 http://dx.doi.org/10.3390/cells8080843 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Shanshan Chen, Muyan Yin, Yingchao Storey, Kenneth B. MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title | MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title_full | MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title_fullStr | MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title_full_unstemmed | MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title_short | MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus |
title_sort | mir-200-3p is potentially involved in cell cycle arrest by regulating cyclin a during aestivation in apostichopus japonicus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721757/ https://www.ncbi.nlm.nih.gov/pubmed/31390757 http://dx.doi.org/10.3390/cells8080843 |
work_keys_str_mv | AT wangshanshan mir2003pispotentiallyinvolvedincellcyclearrestbyregulatingcyclinaduringaestivationinapostichopusjaponicus AT chenmuyan mir2003pispotentiallyinvolvedincellcyclearrestbyregulatingcyclinaduringaestivationinapostichopusjaponicus AT yinyingchao mir2003pispotentiallyinvolvedincellcyclearrestbyregulatingcyclinaduringaestivationinapostichopusjaponicus AT storeykennethb mir2003pispotentiallyinvolvedincellcyclearrestbyregulatingcyclinaduringaestivationinapostichopusjaponicus |