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Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish

Cardiac neural crest cells contribute to important portions of the cardiovascular system including the aorticopulmonary septum and cardiac ganglion. Using replication incompetent avian retroviruses for precise high-resolution lineage analysis, we uncover a previously undescribed neural crest contrib...

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Autores principales: Tang, Weiyi, Martik, Megan L, Li, Yuwei, Bronner, Marianne E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721792/
https://www.ncbi.nlm.nih.gov/pubmed/31393264
http://dx.doi.org/10.7554/eLife.47929
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author Tang, Weiyi
Martik, Megan L
Li, Yuwei
Bronner, Marianne E
author_facet Tang, Weiyi
Martik, Megan L
Li, Yuwei
Bronner, Marianne E
author_sort Tang, Weiyi
collection PubMed
description Cardiac neural crest cells contribute to important portions of the cardiovascular system including the aorticopulmonary septum and cardiac ganglion. Using replication incompetent avian retroviruses for precise high-resolution lineage analysis, we uncover a previously undescribed neural crest contribution to cardiomyocytes of the ventricles in Gallus gallus, supported by Wnt1-Cre lineage analysis in Mus musculus. To test the intriguing possibility that neural crest cells contribute to heart repair, we examined Danio rerio adult heart regeneration in the neural crest transgenic line, Tg(−4.9sox10:eGFP). Whereas the adult heart has few sox10+ cells in the apex, sox10 and other neural crest regulatory network genes are upregulated in the regenerating myocardium after resection. The results suggest that neural crest cells contribute to many cardiovascular structures including cardiomyocytes across vertebrates and to the regenerating heart of teleost fish. Thus, understanding molecular mechanisms that control the normal development of the neural crest into cardiomyocytes and reactivation of the neural crest program upon regeneration may open potential therapeutic approaches to repair heart damage in amniotes.
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spelling pubmed-67217922019-09-05 Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish Tang, Weiyi Martik, Megan L Li, Yuwei Bronner, Marianne E eLife Developmental Biology Cardiac neural crest cells contribute to important portions of the cardiovascular system including the aorticopulmonary septum and cardiac ganglion. Using replication incompetent avian retroviruses for precise high-resolution lineage analysis, we uncover a previously undescribed neural crest contribution to cardiomyocytes of the ventricles in Gallus gallus, supported by Wnt1-Cre lineage analysis in Mus musculus. To test the intriguing possibility that neural crest cells contribute to heart repair, we examined Danio rerio adult heart regeneration in the neural crest transgenic line, Tg(−4.9sox10:eGFP). Whereas the adult heart has few sox10+ cells in the apex, sox10 and other neural crest regulatory network genes are upregulated in the regenerating myocardium after resection. The results suggest that neural crest cells contribute to many cardiovascular structures including cardiomyocytes across vertebrates and to the regenerating heart of teleost fish. Thus, understanding molecular mechanisms that control the normal development of the neural crest into cardiomyocytes and reactivation of the neural crest program upon regeneration may open potential therapeutic approaches to repair heart damage in amniotes. eLife Sciences Publications, Ltd 2019-08-08 /pmc/articles/PMC6721792/ /pubmed/31393264 http://dx.doi.org/10.7554/eLife.47929 Text en © 2019, Tang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Tang, Weiyi
Martik, Megan L
Li, Yuwei
Bronner, Marianne E
Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title_full Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title_fullStr Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title_full_unstemmed Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title_short Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
title_sort cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6721792/
https://www.ncbi.nlm.nih.gov/pubmed/31393264
http://dx.doi.org/10.7554/eLife.47929
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