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Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs

Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicr...

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Autores principales: Farghali, Haithem A., AbdElKader, Naglaa A., AbuBakr, Huda O., Aljuaydi, Samira H., Khattab, Marwa S., Elhelw, Rehab, Elhariri, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722138/
https://www.ncbi.nlm.nih.gov/pubmed/31481694
http://dx.doi.org/10.1038/s41598-019-48657-5
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author Farghali, Haithem A.
AbdElKader, Naglaa A.
AbuBakr, Huda O.
Aljuaydi, Samira H.
Khattab, Marwa S.
Elhelw, Rehab
Elhariri, Mahmoud
author_facet Farghali, Haithem A.
AbdElKader, Naglaa A.
AbuBakr, Huda O.
Aljuaydi, Samira H.
Khattab, Marwa S.
Elhelw, Rehab
Elhariri, Mahmoud
author_sort Farghali, Haithem A.
collection PubMed
description Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicrobial properties of platelet-rich plasma (PRP) against various microorganisms enable its potential use as an alternative to conventional antibiotics. The present work was designed to evaluate the hypothesized antimicrobial activity of PRP against MRSA infected skin wounds. Six adult male dogs were divided equally into control and PRP groups. Unilateral circular full-thickness skin wounds were created then a MRSA suspension was injected locally. Treatment started at 1st week post infection with subcutaneous infiltration of autologous activated PRP every week in the PRP group and with topical application of clindamycin cream twice daily in the control group. PRP decreased wound size and significantly increased wound contractility and re-epithelization, as confirmed by histopathological and immunohistochemical findings. Also PRP treated group showed significant decrease in ROS and redox imbalance with over expression of the TNF-α and VEGFA genes that indicate angiogenesis and maximum antibacterial activity after three weeks. In conclusion, CaCl(2)-activated PRP exhibited antimicrobial activity against MRSA infection, which improved the infected wound healing re-epithelization and granulation tissue formation.
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spelling pubmed-67221382019-09-17 Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs Farghali, Haithem A. AbdElKader, Naglaa A. AbuBakr, Huda O. Aljuaydi, Samira H. Khattab, Marwa S. Elhelw, Rehab Elhariri, Mahmoud Sci Rep Article Effective antimicrobial preparations, other than antibiotics, are important for the treatment of potentially fatal drug-resistant infections. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of hospital-acquired and post- operative infections. Fortunately, the antimicrobial properties of platelet-rich plasma (PRP) against various microorganisms enable its potential use as an alternative to conventional antibiotics. The present work was designed to evaluate the hypothesized antimicrobial activity of PRP against MRSA infected skin wounds. Six adult male dogs were divided equally into control and PRP groups. Unilateral circular full-thickness skin wounds were created then a MRSA suspension was injected locally. Treatment started at 1st week post infection with subcutaneous infiltration of autologous activated PRP every week in the PRP group and with topical application of clindamycin cream twice daily in the control group. PRP decreased wound size and significantly increased wound contractility and re-epithelization, as confirmed by histopathological and immunohistochemical findings. Also PRP treated group showed significant decrease in ROS and redox imbalance with over expression of the TNF-α and VEGFA genes that indicate angiogenesis and maximum antibacterial activity after three weeks. In conclusion, CaCl(2)-activated PRP exhibited antimicrobial activity against MRSA infection, which improved the infected wound healing re-epithelization and granulation tissue formation. Nature Publishing Group UK 2019-09-03 /pmc/articles/PMC6722138/ /pubmed/31481694 http://dx.doi.org/10.1038/s41598-019-48657-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Farghali, Haithem A.
AbdElKader, Naglaa A.
AbuBakr, Huda O.
Aljuaydi, Samira H.
Khattab, Marwa S.
Elhelw, Rehab
Elhariri, Mahmoud
Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title_full Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title_fullStr Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title_full_unstemmed Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title_short Antimicrobial action of autologous platelet-rich plasma on MRSA-infected skin wounds in dogs
title_sort antimicrobial action of autologous platelet-rich plasma on mrsa-infected skin wounds in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722138/
https://www.ncbi.nlm.nih.gov/pubmed/31481694
http://dx.doi.org/10.1038/s41598-019-48657-5
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