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Histological Evidence for Therapeutic Induction of Angiogenesis Using Mast Cells and Platelet-Rich Plasma within A Bioengineered Scaffold following Rat Hindlimb Ischemia
OBJECTIVE: Peripheral arterial disease results from obstructed blood flow in arteries and increases the risk of amputation in acute cases. Therapeutic angiogenesis using bioengineered tissues composed of a chitosan scaffold that was enriched with mast cells (MCs) and/or platelet-rich plasma (PRP) wa...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722454/ https://www.ncbi.nlm.nih.gov/pubmed/31376320 http://dx.doi.org/10.22074/cellj.2020.6287 |
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author | Karimi, Ali Shahrooz, Rasoul Hobbenagh, Rahim Mohammadi, Rahim Delirezh, Nowruz Amani, Saeede Garssen, Johan Mortaz, Esmaeil M Adcock, Ian |
author_facet | Karimi, Ali Shahrooz, Rasoul Hobbenagh, Rahim Mohammadi, Rahim Delirezh, Nowruz Amani, Saeede Garssen, Johan Mortaz, Esmaeil M Adcock, Ian |
author_sort | Karimi, Ali |
collection | PubMed |
description | OBJECTIVE: Peripheral arterial disease results from obstructed blood flow in arteries and increases the risk of amputation in acute cases. Therapeutic angiogenesis using bioengineered tissues composed of a chitosan scaffold that was enriched with mast cells (MCs) and/or platelet-rich plasma (PRP) was used to assess the formation of vascular networks and subsequently improved the functional recovery following hindlimb ischemia. This study aimed to find an optimal approach for restoring local vascularization. MATERIALS AND METHODS: In this experimental study, thirty rats were randomly divided into six experimental groups: a. Ischemic control group with right femoral artery transection, b. Ischemia with phosphate-buffered saline (PBS) control group, c. Ischemia with chitosan scaffold, d. Ischemia with chitosan and MCs, e. Ischemia with chitosan and PRP, and f. Ischemia with chitosan, PRP, and MCs. The left hind limbs served as non-ischemic controls. The analysis of capillary density, arterial diameter, histomorphometric analysis and immunohistochemistry at the transected locations and in gastrocnemius muscles was performed. RESULTS: The group treated with chitosan/MC significantly increased capillary density and the mean number of large blood vessels at the site of femoral artery transection compared with other experimental groups (P<0.05). The treatment with chitosan/MC also significantly increased the muscle fiber diameter and the capillary-to-muscle fiber ratio in gastrocnemius muscles compared with all other ischemic groups (P<0.05). CONCLUSION: These findings suggested that chitosan and MCs together could offer a new approach for the therapeutic induction of angiogenesis in cases of peripheral arterial diseases. |
format | Online Article Text |
id | pubmed-6722454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-67224542020-01-01 Histological Evidence for Therapeutic Induction of Angiogenesis Using Mast Cells and Platelet-Rich Plasma within A Bioengineered Scaffold following Rat Hindlimb Ischemia Karimi, Ali Shahrooz, Rasoul Hobbenagh, Rahim Mohammadi, Rahim Delirezh, Nowruz Amani, Saeede Garssen, Johan Mortaz, Esmaeil M Adcock, Ian Cell J Original Article OBJECTIVE: Peripheral arterial disease results from obstructed blood flow in arteries and increases the risk of amputation in acute cases. Therapeutic angiogenesis using bioengineered tissues composed of a chitosan scaffold that was enriched with mast cells (MCs) and/or platelet-rich plasma (PRP) was used to assess the formation of vascular networks and subsequently improved the functional recovery following hindlimb ischemia. This study aimed to find an optimal approach for restoring local vascularization. MATERIALS AND METHODS: In this experimental study, thirty rats were randomly divided into six experimental groups: a. Ischemic control group with right femoral artery transection, b. Ischemia with phosphate-buffered saline (PBS) control group, c. Ischemia with chitosan scaffold, d. Ischemia with chitosan and MCs, e. Ischemia with chitosan and PRP, and f. Ischemia with chitosan, PRP, and MCs. The left hind limbs served as non-ischemic controls. The analysis of capillary density, arterial diameter, histomorphometric analysis and immunohistochemistry at the transected locations and in gastrocnemius muscles was performed. RESULTS: The group treated with chitosan/MC significantly increased capillary density and the mean number of large blood vessels at the site of femoral artery transection compared with other experimental groups (P<0.05). The treatment with chitosan/MC also significantly increased the muscle fiber diameter and the capillary-to-muscle fiber ratio in gastrocnemius muscles compared with all other ischemic groups (P<0.05). CONCLUSION: These findings suggested that chitosan and MCs together could offer a new approach for the therapeutic induction of angiogenesis in cases of peripheral arterial diseases. Royan Institute 2020 2019-07-31 /pmc/articles/PMC6722454/ /pubmed/31376320 http://dx.doi.org/10.22074/cellj.2020.6287 Text en The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Karimi, Ali Shahrooz, Rasoul Hobbenagh, Rahim Mohammadi, Rahim Delirezh, Nowruz Amani, Saeede Garssen, Johan Mortaz, Esmaeil M Adcock, Ian Histological Evidence for Therapeutic Induction of Angiogenesis Using Mast Cells and Platelet-Rich Plasma within A Bioengineered Scaffold following Rat Hindlimb Ischemia |
title | Histological Evidence for Therapeutic Induction of Angiogenesis
Using Mast Cells and Platelet-Rich Plasma within A
Bioengineered Scaffold following Rat Hindlimb Ischemia |
title_full | Histological Evidence for Therapeutic Induction of Angiogenesis
Using Mast Cells and Platelet-Rich Plasma within A
Bioengineered Scaffold following Rat Hindlimb Ischemia |
title_fullStr | Histological Evidence for Therapeutic Induction of Angiogenesis
Using Mast Cells and Platelet-Rich Plasma within A
Bioengineered Scaffold following Rat Hindlimb Ischemia |
title_full_unstemmed | Histological Evidence for Therapeutic Induction of Angiogenesis
Using Mast Cells and Platelet-Rich Plasma within A
Bioengineered Scaffold following Rat Hindlimb Ischemia |
title_short | Histological Evidence for Therapeutic Induction of Angiogenesis
Using Mast Cells and Platelet-Rich Plasma within A
Bioengineered Scaffold following Rat Hindlimb Ischemia |
title_sort | histological evidence for therapeutic induction of angiogenesis
using mast cells and platelet-rich plasma within a
bioengineered scaffold following rat hindlimb ischemia |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722454/ https://www.ncbi.nlm.nih.gov/pubmed/31376320 http://dx.doi.org/10.22074/cellj.2020.6287 |
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