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Expression of interleukin-6 in ocular surface squamous neoplasia
PURPOSE: Interleukin-6 (IL-6) is an important cytokine in the cascade of inflammation and cancer progression. The aim of this study was to identify IL-6 expression in ocular surface squamous neoplasia (OSSN) in comparison with non-neoplastic conjunctival tissue. METHODS: Twenty paraffin-embedded tis...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722455/ https://www.ncbi.nlm.nih.gov/pubmed/31564816 http://dx.doi.org/10.2147/OPTH.S221911 |
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author | Eghtedari, Masoomeh Beigi, Vahid Maalhagh, Mehrnoosh Ashraf, Hossein |
author_facet | Eghtedari, Masoomeh Beigi, Vahid Maalhagh, Mehrnoosh Ashraf, Hossein |
author_sort | Eghtedari, Masoomeh |
collection | PubMed |
description | PURPOSE: Interleukin-6 (IL-6) is an important cytokine in the cascade of inflammation and cancer progression. The aim of this study was to identify IL-6 expression in ocular surface squamous neoplasia (OSSN) in comparison with non-neoplastic conjunctival tissue. METHODS: Twenty paraffin-embedded tissue sections of conjunctiva from patients with OSSN including conjunctival intraepithelial neoplasia (CIN) in all grades of severity and squamous cell carcinoma (SCC) were assessed by immunohistochemistry staining for IL-6. Twenty non-neoplastic conjunctival sections from age matched patients were selected as the control group. Tissues with more than one focus of inflammatory cell infiltration were excluded from the study. The mean area of positive staining was recorded and the intensity of staining was scored in both groups and compared by statistical methods. RESULTS: The mean staining area in the dysplasia group was significantly more than non-neoplastic conjunctival tissue (63.5±25.96 and 30±15.98 percent respectively; P-value of <0.0001). Nuclear staining was observed in both groups and the difference was not statistically significant. CONCLUSION: IL-6 expressed more in the dysplastic group in compare to non-neoplastic conjunctiva and can therefore be used to diagnose dysplastic state of the conjunctiva; however, in our study, intensity of staining does not correlate with the severity of dysplasia statistically; most probably because of a low sample size in each category. The role of nuclear staining is not clear. Our findings can be an introduction toward targeted treatment of ocular surface neoplasia by the aim of newer anti-IL agents. Further investigation is needed. |
format | Online Article Text |
id | pubmed-6722455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-67224552019-09-27 Expression of interleukin-6 in ocular surface squamous neoplasia Eghtedari, Masoomeh Beigi, Vahid Maalhagh, Mehrnoosh Ashraf, Hossein Clin Ophthalmol Original Research PURPOSE: Interleukin-6 (IL-6) is an important cytokine in the cascade of inflammation and cancer progression. The aim of this study was to identify IL-6 expression in ocular surface squamous neoplasia (OSSN) in comparison with non-neoplastic conjunctival tissue. METHODS: Twenty paraffin-embedded tissue sections of conjunctiva from patients with OSSN including conjunctival intraepithelial neoplasia (CIN) in all grades of severity and squamous cell carcinoma (SCC) were assessed by immunohistochemistry staining for IL-6. Twenty non-neoplastic conjunctival sections from age matched patients were selected as the control group. Tissues with more than one focus of inflammatory cell infiltration were excluded from the study. The mean area of positive staining was recorded and the intensity of staining was scored in both groups and compared by statistical methods. RESULTS: The mean staining area in the dysplasia group was significantly more than non-neoplastic conjunctival tissue (63.5±25.96 and 30±15.98 percent respectively; P-value of <0.0001). Nuclear staining was observed in both groups and the difference was not statistically significant. CONCLUSION: IL-6 expressed more in the dysplastic group in compare to non-neoplastic conjunctiva and can therefore be used to diagnose dysplastic state of the conjunctiva; however, in our study, intensity of staining does not correlate with the severity of dysplasia statistically; most probably because of a low sample size in each category. The role of nuclear staining is not clear. Our findings can be an introduction toward targeted treatment of ocular surface neoplasia by the aim of newer anti-IL agents. Further investigation is needed. Dove 2019-08-30 /pmc/articles/PMC6722455/ /pubmed/31564816 http://dx.doi.org/10.2147/OPTH.S221911 Text en © 2019 Eghtedari et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Eghtedari, Masoomeh Beigi, Vahid Maalhagh, Mehrnoosh Ashraf, Hossein Expression of interleukin-6 in ocular surface squamous neoplasia |
title | Expression of interleukin-6 in ocular surface squamous neoplasia |
title_full | Expression of interleukin-6 in ocular surface squamous neoplasia |
title_fullStr | Expression of interleukin-6 in ocular surface squamous neoplasia |
title_full_unstemmed | Expression of interleukin-6 in ocular surface squamous neoplasia |
title_short | Expression of interleukin-6 in ocular surface squamous neoplasia |
title_sort | expression of interleukin-6 in ocular surface squamous neoplasia |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722455/ https://www.ncbi.nlm.nih.gov/pubmed/31564816 http://dx.doi.org/10.2147/OPTH.S221911 |
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