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Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4

Retinol binding protein 4 (RBP4), mainly secreted by the liver and adipocytes, is a transporter of vitamin A. RBP4 has been shown to be involved in several pathophysiological processes, such as obesity, insulin resistance, and cardiovascular risk. Reports have indicated the high expression levels of...

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Autores principales: Zhao, Yun, Li, Chunjin, Zhou, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722559/
https://www.ncbi.nlm.nih.gov/pubmed/31412686
http://dx.doi.org/10.3390/genes10080615
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author Zhao, Yun
Li, Chunjin
Zhou, Xu
author_facet Zhao, Yun
Li, Chunjin
Zhou, Xu
author_sort Zhao, Yun
collection PubMed
description Retinol binding protein 4 (RBP4), mainly secreted by the liver and adipocytes, is a transporter of vitamin A. RBP4 has been shown to be involved in several pathophysiological processes, such as obesity, insulin resistance, and cardiovascular risk. Reports have indicated the high expression levels of RBP4 in cystic follicles. However, the role of RBP4 in mammalian follicular granulosa cells (GCs) remains largely unknown. To illustrate the molecular pathways associated with the effects of RBP4 on GCs, we used high-throughput sequencing to detect differential gene expression in GCs overexpressing RBP4. A total of 113 differentially expressed genes (DEGs) were identified in RBP4-overexpressing GCs, and they included 71 upregulated and 42 downregulated genes. The differential expressions of the top 10 DEGs were further confirmed by real-time quantitative polymerase chain reaction. Pathway analysis indicated that the DEGs are mostly involved in oxidative phosphorylation, Parkinson’s disease, non-alcoholic fatty liver disease, Huntington’s disease, cardiac muscle contraction, Alzheimer’s disease, fatty acid biosynthesis, AMP-activated protein kinase signaling pathway, and insulin signaling pathway. Genes in these pathways should be useful for future studies on GCs. Altogether, the results of our study establish a framework for understanding the potential functions of RBP4 in porcine GCs.
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spelling pubmed-67225592019-09-10 Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4 Zhao, Yun Li, Chunjin Zhou, Xu Genes (Basel) Article Retinol binding protein 4 (RBP4), mainly secreted by the liver and adipocytes, is a transporter of vitamin A. RBP4 has been shown to be involved in several pathophysiological processes, such as obesity, insulin resistance, and cardiovascular risk. Reports have indicated the high expression levels of RBP4 in cystic follicles. However, the role of RBP4 in mammalian follicular granulosa cells (GCs) remains largely unknown. To illustrate the molecular pathways associated with the effects of RBP4 on GCs, we used high-throughput sequencing to detect differential gene expression in GCs overexpressing RBP4. A total of 113 differentially expressed genes (DEGs) were identified in RBP4-overexpressing GCs, and they included 71 upregulated and 42 downregulated genes. The differential expressions of the top 10 DEGs were further confirmed by real-time quantitative polymerase chain reaction. Pathway analysis indicated that the DEGs are mostly involved in oxidative phosphorylation, Parkinson’s disease, non-alcoholic fatty liver disease, Huntington’s disease, cardiac muscle contraction, Alzheimer’s disease, fatty acid biosynthesis, AMP-activated protein kinase signaling pathway, and insulin signaling pathway. Genes in these pathways should be useful for future studies on GCs. Altogether, the results of our study establish a framework for understanding the potential functions of RBP4 in porcine GCs. MDPI 2019-08-13 /pmc/articles/PMC6722559/ /pubmed/31412686 http://dx.doi.org/10.3390/genes10080615 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Yun
Li, Chunjin
Zhou, Xu
Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title_full Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title_fullStr Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title_full_unstemmed Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title_short Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4
title_sort transcriptomic analysis of porcine granulosa cells overexpressing retinol binding protein 4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722559/
https://www.ncbi.nlm.nih.gov/pubmed/31412686
http://dx.doi.org/10.3390/genes10080615
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