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Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules

For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main nega...

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Autor principal: Blázquez-Castro, Alfonso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722566/
https://www.ncbi.nlm.nih.gov/pubmed/31370251
http://dx.doi.org/10.3390/mi10080507
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author Blázquez-Castro, Alfonso
author_facet Blázquez-Castro, Alfonso
author_sort Blázquez-Castro, Alfonso
collection PubMed
description For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main negative effects of optical tweezers upon biostructures and living systems will be presented. There are three main areas on which the review will focus: linear optical excitation within the tweezers, non-linear photonic effects, and thermal load upon the sampled volume. Additional information is provided on negative mechanical effects of optical traps on biological structures. Strategies to avoid or, at least, minimize these negative effects will be introduced. Finally, all these effects, undesirable for the most, can have positive applications under the right conditions. Some hints in this direction will also be discussed.
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spelling pubmed-67225662019-09-10 Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules Blázquez-Castro, Alfonso Micromachines (Basel) Review For several decades optical tweezers have proven to be an invaluable tool in the study and analysis of myriad biological responses and applications. However, as with every tool, they can have undesirable or damaging effects upon the very sample they are helping to study. In this review the main negative effects of optical tweezers upon biostructures and living systems will be presented. There are three main areas on which the review will focus: linear optical excitation within the tweezers, non-linear photonic effects, and thermal load upon the sampled volume. Additional information is provided on negative mechanical effects of optical traps on biological structures. Strategies to avoid or, at least, minimize these negative effects will be introduced. Finally, all these effects, undesirable for the most, can have positive applications under the right conditions. Some hints in this direction will also be discussed. MDPI 2019-07-31 /pmc/articles/PMC6722566/ /pubmed/31370251 http://dx.doi.org/10.3390/mi10080507 Text en © 2019 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Blázquez-Castro, Alfonso
Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title_full Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title_fullStr Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title_full_unstemmed Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title_short Optical Tweezers: Phototoxicity and Thermal Stress in Cells and Biomolecules
title_sort optical tweezers: phototoxicity and thermal stress in cells and biomolecules
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6722566/
https://www.ncbi.nlm.nih.gov/pubmed/31370251
http://dx.doi.org/10.3390/mi10080507
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