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Contrast of Real-Time Fluorescent PCR Methods for Detection of Escherichia coli O157:H7 and of Introducing an Internal Amplification Control

Various constituents in food specimens can inhibit the PCR assay and lead to false-negative results. An internal amplification control was employed to monitor the presence of false-negative results in PCR amplification. In this study, the objectives were to compare the real-time PCR-based method by...

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Detalles Bibliográficos
Autores principales: Zhao, Xihong, Xia, Jing, Liu, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723022/
https://www.ncbi.nlm.nih.gov/pubmed/31370338
http://dx.doi.org/10.3390/microorganisms7080230