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Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples

The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among...

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Autores principales: Maikanov, Balgabay, Mustafina, Raikhan, Auteleyeva, Laura, Wiśniewski, Jan, Anusz, Krzysztof, Grenda, Tomasz, Kwiatek, Krzysztof, Goldsztejn, Magdalena, Grabczak, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723067/
https://www.ncbi.nlm.nih.gov/pubmed/31412583
http://dx.doi.org/10.3390/toxins11080472
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author Maikanov, Balgabay
Mustafina, Raikhan
Auteleyeva, Laura
Wiśniewski, Jan
Anusz, Krzysztof
Grenda, Tomasz
Kwiatek, Krzysztof
Goldsztejn, Magdalena
Grabczak, Magdalena
author_facet Maikanov, Balgabay
Mustafina, Raikhan
Auteleyeva, Laura
Wiśniewski, Jan
Anusz, Krzysztof
Grenda, Tomasz
Kwiatek, Krzysztof
Goldsztejn, Magdalena
Grabczak, Magdalena
author_sort Maikanov, Balgabay
collection PubMed
description The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey.
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spelling pubmed-67230672019-09-10 Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples Maikanov, Balgabay Mustafina, Raikhan Auteleyeva, Laura Wiśniewski, Jan Anusz, Krzysztof Grenda, Tomasz Kwiatek, Krzysztof Goldsztejn, Magdalena Grabczak, Magdalena Toxins (Basel) Communication The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey. MDPI 2019-08-13 /pmc/articles/PMC6723067/ /pubmed/31412583 http://dx.doi.org/10.3390/toxins11080472 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Maikanov, Balgabay
Mustafina, Raikhan
Auteleyeva, Laura
Wiśniewski, Jan
Anusz, Krzysztof
Grenda, Tomasz
Kwiatek, Krzysztof
Goldsztejn, Magdalena
Grabczak, Magdalena
Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title_full Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title_fullStr Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title_full_unstemmed Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title_short Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
title_sort clostridium botulinum and clostridium perfringens occurrence in kazakh honey samples
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723067/
https://www.ncbi.nlm.nih.gov/pubmed/31412583
http://dx.doi.org/10.3390/toxins11080472
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