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Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples
The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723067/ https://www.ncbi.nlm.nih.gov/pubmed/31412583 http://dx.doi.org/10.3390/toxins11080472 |
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author | Maikanov, Balgabay Mustafina, Raikhan Auteleyeva, Laura Wiśniewski, Jan Anusz, Krzysztof Grenda, Tomasz Kwiatek, Krzysztof Goldsztejn, Magdalena Grabczak, Magdalena |
author_facet | Maikanov, Balgabay Mustafina, Raikhan Auteleyeva, Laura Wiśniewski, Jan Anusz, Krzysztof Grenda, Tomasz Kwiatek, Krzysztof Goldsztejn, Magdalena Grabczak, Magdalena |
author_sort | Maikanov, Balgabay |
collection | PubMed |
description | The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey. |
format | Online Article Text |
id | pubmed-6723067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67230672019-09-10 Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples Maikanov, Balgabay Mustafina, Raikhan Auteleyeva, Laura Wiśniewski, Jan Anusz, Krzysztof Grenda, Tomasz Kwiatek, Krzysztof Goldsztejn, Magdalena Grabczak, Magdalena Toxins (Basel) Communication The aim of this study was to assess occurrence of Clostridium botulinum and Clostridium perfringens in honey samples from Kazakhstan. Analyses were carried out using a set of PCR methods for identification of anaerobic bacteria, and detection of toxin genes of C. botulinum and C. perfringens. Among 197 samples, C. botulinum was noticed in only one (0.5%). The isolated strain of this pathogen showed the presence of the bont/A and ntnh genes. C. perfringens strains were isolated from 18 (9%) samples, and mPCR (multiplex PCR) analysis led to them all being classified as toxin type A with the ability to produce α toxin. Sequence analysis of 16S rDNA genes showed occurrence in 4 samples of other anaerobes related to C. botulinum, which were C. sporogenes and C. beijerinckii strains. C. botulinum prevalence in honey samples from Kazakhstan in comparison to the prevalence in samples collected from the other regions seems to be less. The highest prevalence of Clostridium sp. was noticed in the East Kazakhstan province. Our study is the first survey on BoNT-producing clostridia and C. perfringens prevalence in Kazakh honey. MDPI 2019-08-13 /pmc/articles/PMC6723067/ /pubmed/31412583 http://dx.doi.org/10.3390/toxins11080472 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Maikanov, Balgabay Mustafina, Raikhan Auteleyeva, Laura Wiśniewski, Jan Anusz, Krzysztof Grenda, Tomasz Kwiatek, Krzysztof Goldsztejn, Magdalena Grabczak, Magdalena Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title | Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title_full | Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title_fullStr | Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title_full_unstemmed | Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title_short | Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples |
title_sort | clostridium botulinum and clostridium perfringens occurrence in kazakh honey samples |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723067/ https://www.ncbi.nlm.nih.gov/pubmed/31412583 http://dx.doi.org/10.3390/toxins11080472 |
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