Cargando…

Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti

The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental desig...

Descripción completa

Detalles Bibliográficos
Autores principales: Boullis, Antoine, Cordel, Nadège, Herrmann-Storck, Cécile, Vega-Rúa, Anubis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723193/
https://www.ncbi.nlm.nih.gov/pubmed/31370135
http://dx.doi.org/10.3390/v11080695
_version_ 1783448712423407616
author Boullis, Antoine
Cordel, Nadège
Herrmann-Storck, Cécile
Vega-Rúa, Anubis
author_facet Boullis, Antoine
Cordel, Nadège
Herrmann-Storck, Cécile
Vega-Rúa, Anubis
author_sort Boullis, Antoine
collection PubMed
description The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental design. Mosquitoes were allowed to feed on ZIKV-infected blood and were then rapidly transferred to feed on ZIKV-free blood until they finished their meal. The uninfected blood meals, the mosquito abdomens, as well as the mouthparts dissected from fully and partially engorged mosquitoes were analyzed using RT-qPCR and/or virus titration. All the fully engorged mosquito abdomens were ZIKV-infected, whereas their mouthparts were all ZIKV-negative. Nonetheless, one of the partially engorged mosquitoes carried infectious particles on mouthparts. No infectious virus was found in the receiver blood meals, while viral RNA was detected in 9% of the samples (2/22). Thus, mechanical transmission of ZIKV may sporadically occur via Ae. aegypti bite. However, as the number of virions detected on mouthparts (2 particles) is not sufficient to induce infection in a naïve host, our results indicate that mechanical transmission does not impact ZIKV epidemiology.
format Online
Article
Text
id pubmed-6723193
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67231932019-09-10 Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti Boullis, Antoine Cordel, Nadège Herrmann-Storck, Cécile Vega-Rúa, Anubis Viruses Article The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental design. Mosquitoes were allowed to feed on ZIKV-infected blood and were then rapidly transferred to feed on ZIKV-free blood until they finished their meal. The uninfected blood meals, the mosquito abdomens, as well as the mouthparts dissected from fully and partially engorged mosquitoes were analyzed using RT-qPCR and/or virus titration. All the fully engorged mosquito abdomens were ZIKV-infected, whereas their mouthparts were all ZIKV-negative. Nonetheless, one of the partially engorged mosquitoes carried infectious particles on mouthparts. No infectious virus was found in the receiver blood meals, while viral RNA was detected in 9% of the samples (2/22). Thus, mechanical transmission of ZIKV may sporadically occur via Ae. aegypti bite. However, as the number of virions detected on mouthparts (2 particles) is not sufficient to induce infection in a naïve host, our results indicate that mechanical transmission does not impact ZIKV epidemiology. MDPI 2019-07-31 /pmc/articles/PMC6723193/ /pubmed/31370135 http://dx.doi.org/10.3390/v11080695 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boullis, Antoine
Cordel, Nadège
Herrmann-Storck, Cécile
Vega-Rúa, Anubis
Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title_full Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title_fullStr Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title_full_unstemmed Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title_short Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
title_sort experimental assessment of zika virus mechanical transmission by aedes aegypti
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723193/
https://www.ncbi.nlm.nih.gov/pubmed/31370135
http://dx.doi.org/10.3390/v11080695
work_keys_str_mv AT boullisantoine experimentalassessmentofzikavirusmechanicaltransmissionbyaedesaegypti
AT cordelnadege experimentalassessmentofzikavirusmechanicaltransmissionbyaedesaegypti
AT herrmannstorckcecile experimentalassessmentofzikavirusmechanicaltransmissionbyaedesaegypti
AT vegaruaanubis experimentalassessmentofzikavirusmechanicaltransmissionbyaedesaegypti