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Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti
The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental desig...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723193/ https://www.ncbi.nlm.nih.gov/pubmed/31370135 http://dx.doi.org/10.3390/v11080695 |
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author | Boullis, Antoine Cordel, Nadège Herrmann-Storck, Cécile Vega-Rúa, Anubis |
author_facet | Boullis, Antoine Cordel, Nadège Herrmann-Storck, Cécile Vega-Rúa, Anubis |
author_sort | Boullis, Antoine |
collection | PubMed |
description | The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental design. Mosquitoes were allowed to feed on ZIKV-infected blood and were then rapidly transferred to feed on ZIKV-free blood until they finished their meal. The uninfected blood meals, the mosquito abdomens, as well as the mouthparts dissected from fully and partially engorged mosquitoes were analyzed using RT-qPCR and/or virus titration. All the fully engorged mosquito abdomens were ZIKV-infected, whereas their mouthparts were all ZIKV-negative. Nonetheless, one of the partially engorged mosquitoes carried infectious particles on mouthparts. No infectious virus was found in the receiver blood meals, while viral RNA was detected in 9% of the samples (2/22). Thus, mechanical transmission of ZIKV may sporadically occur via Ae. aegypti bite. However, as the number of virions detected on mouthparts (2 particles) is not sufficient to induce infection in a naïve host, our results indicate that mechanical transmission does not impact ZIKV epidemiology. |
format | Online Article Text |
id | pubmed-6723193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67231932019-09-10 Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti Boullis, Antoine Cordel, Nadège Herrmann-Storck, Cécile Vega-Rúa, Anubis Viruses Article The pandemic emergence of several mosquito-borne viruses highlights the need to understand the different ways in which they can be transmitted by vectors to human hosts. In this study, we evaluated the propensity of Aedes aegypti to transmit mechanically Zika virus (ZIKV) using an experimental design. Mosquitoes were allowed to feed on ZIKV-infected blood and were then rapidly transferred to feed on ZIKV-free blood until they finished their meal. The uninfected blood meals, the mosquito abdomens, as well as the mouthparts dissected from fully and partially engorged mosquitoes were analyzed using RT-qPCR and/or virus titration. All the fully engorged mosquito abdomens were ZIKV-infected, whereas their mouthparts were all ZIKV-negative. Nonetheless, one of the partially engorged mosquitoes carried infectious particles on mouthparts. No infectious virus was found in the receiver blood meals, while viral RNA was detected in 9% of the samples (2/22). Thus, mechanical transmission of ZIKV may sporadically occur via Ae. aegypti bite. However, as the number of virions detected on mouthparts (2 particles) is not sufficient to induce infection in a naïve host, our results indicate that mechanical transmission does not impact ZIKV epidemiology. MDPI 2019-07-31 /pmc/articles/PMC6723193/ /pubmed/31370135 http://dx.doi.org/10.3390/v11080695 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Boullis, Antoine Cordel, Nadège Herrmann-Storck, Cécile Vega-Rúa, Anubis Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title | Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title_full | Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title_fullStr | Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title_full_unstemmed | Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title_short | Experimental Assessment of Zika Virus Mechanical Transmission by Aedes aegypti |
title_sort | experimental assessment of zika virus mechanical transmission by aedes aegypti |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723193/ https://www.ncbi.nlm.nih.gov/pubmed/31370135 http://dx.doi.org/10.3390/v11080695 |
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