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Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)

The ability of the isolate VG008 of S. frugiperda granulovirus (SpfrGV) to enhance the infectivity of the isolate SfCOL of S. frugiperda multiple nucleopolyhedrovirus (SpfrMNPV) was evaluated on S. frugiperda larvae. Bioassays were performed with mixtures by using different proportions 90%:10% (M1),...

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Autores principales: Cuartas-Otálora, Paola E., Gómez-Valderrama, Juliana A., Ramos, Andrea E., Barrera-Cubillos, Gloria P., Villamizar-Rivero, Laura F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723348/
https://www.ncbi.nlm.nih.gov/pubmed/31357428
http://dx.doi.org/10.3390/v11080684
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author Cuartas-Otálora, Paola E.
Gómez-Valderrama, Juliana A.
Ramos, Andrea E.
Barrera-Cubillos, Gloria P.
Villamizar-Rivero, Laura F.
author_facet Cuartas-Otálora, Paola E.
Gómez-Valderrama, Juliana A.
Ramos, Andrea E.
Barrera-Cubillos, Gloria P.
Villamizar-Rivero, Laura F.
author_sort Cuartas-Otálora, Paola E.
collection PubMed
description The ability of the isolate VG008 of S. frugiperda granulovirus (SpfrGV) to enhance the infectivity of the isolate SfCOL of S. frugiperda multiple nucleopolyhedrovirus (SpfrMNPV) was evaluated on S. frugiperda larvae. Bioassays were performed with mixtures by using different proportions 90%:10% (M1), 95%:5% (M2) and 97.5%:2.5% (M3) of SfCOL:VG008, respectively. All mixtures showed higher insecticidal activity that SfCOL. The mixture M3 showed the highest enhancement of SfCOL reducing 11.40 times the Mean Lethal Concentration and 96 h in the Mean Time to Death. The enhancer activity of proteins derived from VG008 (GVPs) were also evaluated in mixture with SfCOL. The GVPs increased 27% larval mortality caused by SfCOL and damaged the peritrophic membrane of S. litura larvae, suggesting that the key point in this enhancing activity is the initial step of the larva colonization, the midgut infection. M3 was formulated and evaluated under greenhouse conditions in maize plants using different doses. The highest efficacy was obtained with the highest dose of M3 (8 × 10(11) OBs/ha), which was similar to that found when formulated SfCOL was applied using an approximately twofold higher dose. The viral mixture M3 was selected as the active ingredient for developing a new biopesticide for a more efficient management of the pest in the field.
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spelling pubmed-67233482019-09-10 Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) Cuartas-Otálora, Paola E. Gómez-Valderrama, Juliana A. Ramos, Andrea E. Barrera-Cubillos, Gloria P. Villamizar-Rivero, Laura F. Viruses Article The ability of the isolate VG008 of S. frugiperda granulovirus (SpfrGV) to enhance the infectivity of the isolate SfCOL of S. frugiperda multiple nucleopolyhedrovirus (SpfrMNPV) was evaluated on S. frugiperda larvae. Bioassays were performed with mixtures by using different proportions 90%:10% (M1), 95%:5% (M2) and 97.5%:2.5% (M3) of SfCOL:VG008, respectively. All mixtures showed higher insecticidal activity that SfCOL. The mixture M3 showed the highest enhancement of SfCOL reducing 11.40 times the Mean Lethal Concentration and 96 h in the Mean Time to Death. The enhancer activity of proteins derived from VG008 (GVPs) were also evaluated in mixture with SfCOL. The GVPs increased 27% larval mortality caused by SfCOL and damaged the peritrophic membrane of S. litura larvae, suggesting that the key point in this enhancing activity is the initial step of the larva colonization, the midgut infection. M3 was formulated and evaluated under greenhouse conditions in maize plants using different doses. The highest efficacy was obtained with the highest dose of M3 (8 × 10(11) OBs/ha), which was similar to that found when formulated SfCOL was applied using an approximately twofold higher dose. The viral mixture M3 was selected as the active ingredient for developing a new biopesticide for a more efficient management of the pest in the field. MDPI 2019-07-26 /pmc/articles/PMC6723348/ /pubmed/31357428 http://dx.doi.org/10.3390/v11080684 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cuartas-Otálora, Paola E.
Gómez-Valderrama, Juliana A.
Ramos, Andrea E.
Barrera-Cubillos, Gloria P.
Villamizar-Rivero, Laura F.
Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title_full Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title_fullStr Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title_full_unstemmed Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title_short Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)
title_sort bio-insecticidal potential of nucleopolyhedrovirus and granulovirus mixtures to control the fall armyworm spodoptera frugiperda (j.e. smith, 1797) (lepidoptera: noctuidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723348/
https://www.ncbi.nlm.nih.gov/pubmed/31357428
http://dx.doi.org/10.3390/v11080684
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