Cargando…

Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn

Fusarium species coexist as toxigenic, systemic pathogens in sweet corn seed production in southwestern Idaho, USA. We hypothesized that fungal antagonists of seedborne Fusarium would differentially alter production of Fusarium mycotoxins directly and/or systemically. We challenged the Fusarium comp...

Descripción completa

Detalles Bibliográficos
Autores principales: Ridout, Mary E., Godfrey, Bruce, Newcombe, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723472/
https://www.ncbi.nlm.nih.gov/pubmed/31349594
http://dx.doi.org/10.3390/toxins11080438
_version_ 1783448776552218624
author Ridout, Mary E.
Godfrey, Bruce
Newcombe, George
author_facet Ridout, Mary E.
Godfrey, Bruce
Newcombe, George
author_sort Ridout, Mary E.
collection PubMed
description Fusarium species coexist as toxigenic, systemic pathogens in sweet corn seed production in southwestern Idaho, USA. We hypothesized that fungal antagonists of seedborne Fusarium would differentially alter production of Fusarium mycotoxins directly and/or systemically. We challenged the Fusarium complex by in vitro antagonism trials and in situ silk and seed inoculations with fungal antagonists. Fungal antagonists reduced growth and sporulation of Fusarium species in vitro from 40.5% to as much as 100%. Pichia membranifaciens and Penicillium griseolum reduced fumonisin production by F. verticillioides by 73% and 49%, respectively, while P. membranifaciens and a novel Penicillium sp. (WPT) reduced fumonisins by F. proliferatum 56% and 78%, respectively. In situ, pre-planting inoculation of seeds with Penicillium WPT systemically increased fumonisins in the resulting crop. Morchella snyderi applied to silks of an F1 cross systemically reduced deoxynivalenol by 47% in mature seeds of the F2. Antagonists failed to suppress Fusarium in mature kernels following silk inoculations, although the ratio of F. verticillioides to total Fusarium double with some inoculants. Fusarium mycotoxin concentrations in sweet corn seed change systemically, as well as locally, in response to the presence of fungal antagonists, although in Fusarium presence in situ was not changed.
format Online
Article
Text
id pubmed-6723472
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67234722019-09-10 Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn Ridout, Mary E. Godfrey, Bruce Newcombe, George Toxins (Basel) Article Fusarium species coexist as toxigenic, systemic pathogens in sweet corn seed production in southwestern Idaho, USA. We hypothesized that fungal antagonists of seedborne Fusarium would differentially alter production of Fusarium mycotoxins directly and/or systemically. We challenged the Fusarium complex by in vitro antagonism trials and in situ silk and seed inoculations with fungal antagonists. Fungal antagonists reduced growth and sporulation of Fusarium species in vitro from 40.5% to as much as 100%. Pichia membranifaciens and Penicillium griseolum reduced fumonisin production by F. verticillioides by 73% and 49%, respectively, while P. membranifaciens and a novel Penicillium sp. (WPT) reduced fumonisins by F. proliferatum 56% and 78%, respectively. In situ, pre-planting inoculation of seeds with Penicillium WPT systemically increased fumonisins in the resulting crop. Morchella snyderi applied to silks of an F1 cross systemically reduced deoxynivalenol by 47% in mature seeds of the F2. Antagonists failed to suppress Fusarium in mature kernels following silk inoculations, although the ratio of F. verticillioides to total Fusarium double with some inoculants. Fusarium mycotoxin concentrations in sweet corn seed change systemically, as well as locally, in response to the presence of fungal antagonists, although in Fusarium presence in situ was not changed. MDPI 2019-07-25 /pmc/articles/PMC6723472/ /pubmed/31349594 http://dx.doi.org/10.3390/toxins11080438 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ridout, Mary E.
Godfrey, Bruce
Newcombe, George
Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title_full Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title_fullStr Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title_full_unstemmed Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title_short Effects of Antagonists on Mycotoxins of Seedborne Fusarium spp. in Sweet Corn
title_sort effects of antagonists on mycotoxins of seedborne fusarium spp. in sweet corn
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723472/
https://www.ncbi.nlm.nih.gov/pubmed/31349594
http://dx.doi.org/10.3390/toxins11080438
work_keys_str_mv AT ridoutmarye effectsofantagonistsonmycotoxinsofseedbornefusariumsppinsweetcorn
AT godfreybruce effectsofantagonistsonmycotoxinsofseedbornefusariumsppinsweetcorn
AT newcombegeorge effectsofantagonistsonmycotoxinsofseedbornefusariumsppinsweetcorn